Top

Journal of Sol-Gel Science and Technology

Published in:

25-10-2023 | Original Paper

Synthesis and characterization of bioactive silver nanoparticles: evaluation of their anticancer potential against MCF7 cell line and antibacterial activity against foodborne pathogens

Authors: A. Saravanan, V. Parthasarathy, P. Senthil Kumar

Published in: Journal of Sol-Gel Science and Technology | Issue 1/2024

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

The bioactive silver nanoparticles (SNPs) were synthesized using leaf extract of Leucas zeylanica (LZ) as a bioreductant. The polyphenols of LZ extract were involved in stabilizing bioactive SNPs. The biomolecules of LZ were acting as capping and stabilizing agents. The physical interaction of SNPs with the biomolecules of LZ was concluded by FTIR. The structure of SNPs was affirmed by XRD, and the particle size was obtained as 46 nm using Scherer’s equation. The UV–Visible analysis revealed that the SPR peak appeared at 422 nm only after the bio-reduction of Ag⁺ ions into SNPs. The LZ-capped SNPs were also investigated to evaluate their anticancer activity against MCF7 cells. The prepared SNPs induced cell death of 91% at a concentration of 1000 µg/ml. The antimicrobial potential of SNPs was evaluated against four foodborne pathogenic strains by the disk diffusion method. The bioactive SNPs exhibited the highest inhibition zone of 42 mm against S. aureus. The brine shrimp was employed as a bioassay to assess the cytotoxicity of the LZ-mediated colloidal SNPs.

Graphical abstract

previous article Synthesis of zeolitic imidazolate framework-67 aerogel by anion exchange

next article Preparation and properties of PDMS elastomer cross-linked with hydrolyzate of tetraethoxysilane, hexaethoxydisiloxane, and octaethoxytrisiloxane: influence of cross-linker structure

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Riaz M, Mutreja V, Sareen S, Ahmad B, Faheem N, Zahid N, Jabbour G, Park J (2021) Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature. Sci Rep 11:2866. https://www.nature.com/articles/s41598-021-82555-zCrossRef

Jain PK, Huang X, El-Sayed IH, Noble MA (2008) Nobel metals on the nanoscale: optical and photothermal properties and some applications in imaging, sensing, biology, and medicine. Acc Chem Res 41:1578–1586. https://doi.org/10.1021/ar7002804CrossRef

Sashikiran P, Susmila AG, Venkata Subbaiah K, Josthna P, Varadarajulu NC (2021) Biosynthesis of silver nanoparticles using leaf extract of Decaschistia crotonifolia and its antibacterial, antioxidant, and catalytic applications. Green Chem Lett Rev 14:137–152. https://doi.org/10.1080/17518253.2021.1876172CrossRef

Almatroudi A (2020) Silver nanoparticles: synthesis characterization and biomedical applications. Open Life Sci 15(1):819–839. https://pubmed.ncbi.nlm.nih.gov/33817269CrossRef

Gehrke I, Geiser A, Somborn-Schulz A (2015) Innovations in nanotechnology for water treatment. Nanotechnol Sci Appl 8:1–17. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294021CrossRef

Jasmani L, Rusli R, Khadiran T et al. (2020) Application of nanotechnology in wood based industry. Nanoscale Res Lett 15:207. https://doi.org/10.1186/s11671-020-03438-2#article-infoCrossRef

Zhang X, Liu Z, Shen W, Gurunathan S (2016) Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci 17(9):1534. https://doi.org/10.3390/ijms17091534CrossRef

Wilkinson LJ, White RJ, Chipman JK (2011) Silver and nanoparticles of silver in wound dressings a review of efficacy and safety. J Wound Care 20(11):543–549. https://pubmed.ncbi.nlm.nih.gov/22240850/CrossRef

Akhavan O, Ghaderi E, Akhavan A (2012) Silver dependent genotoxiciy of graphene nano platelets in human stem cells. Biomaterials 33:8017–8025. https://pubmed.ncbi.nlm.nih.gov/22863381/CrossRef

10.

Akhavan O, Ghaderi E, Abouei E, Hatamie S, Ghasemi E (2014) Accelerated differentiation of neural stem cells into neurons on ginseng-reduced graphene oxide sheets. Carbon 66:395–406. https://doi.org/10.1016/j.carbon.2013.09.015CrossRef

11.

Jain S, Mehata MS (2017) Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci Rep 7:15867. https://www.nature.com/articles/s41598-017-15724-8CrossRef

12.

Verma A, Mehata MS (2016) Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. J Rad Res Appl Sci 9:109–115. https://www.sciencedirect.com/science/article/pii/S168785071500120X

13.

Vanaraj S, Keerthana BB, Preethi K (2017) Biosynthesis, characterization of silver nanoparticles using quercetin from Clitoria ternatea L to enhance toxicity against bacterial biofilm. J Inorg Organomet Polym 27:1412–1422. https://doi.org/10.1007/s10904-017-0595-8CrossRef

14.

Priya B, Mantosh S, Aniruddha M, Papita D (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess, 1:3. https://doi.org/10.1186/s40643-014-0003-yCrossRef

15.

Krithiga N, Rajalakshmi A, Jayachitra A et al. (2015) Green synthesis of silver nanoparticles using leaf extracts of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci 2015:928204. https://www.hindawi.com/journals/jns/2015/928204/CrossRef

16.

Khalir WKA, Shameli K et al. (2020) Biosynthesized silver nanoparticles by aqueous stem extract of entada spiralis and screening of their biomedical activity. Front Chem 8:620. https://doi.org/10.3389/fchem.2020.00620CrossRef

17.

Alsammarraie FK, Wang W, Zhou P et al. (2018) Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids Surf B Biointerfaces 171:398–405. https://doi.org/10.1016/j.colsurfb.2018.07.059CrossRef

18.

Kumar SSD, Houreld NN, Kroukamp EM, Abrahamse H (2018) Cellular imaging and bactericidal mechanism of green-synthesized silver nanoparticles against human pathogenic bacteria. J Photochem Photobiol B Biol 178:259–269. https://pubmed.ncbi.nlm.nih.gov/29172133/CrossRef

19.

Labulo AH, Adesuji ET et al. (2016) A dual-purpose silver nanoparticles biosynthesized using aqueous leaf extract of Detarium microcarpum: an under-utilized species. Talanta 160:735–744. https://pubmed.ncbi.nlm.nih.gov/27591670/CrossRef

20.

Maddinedi SB, Mandal BK, Maddili SKS (2017) Biofabrication of size controllable silver nanoparticles—a green approach. J Photochem Photobiol B Biol 167:236–241. https://pubmed.ncbi.nlm.nih.gov/28088104/CrossRef

21.

Paosen S, Saising J, Septama A, Voravuthikunchai S (2017) Green synthesis of silver nanoparticles using plants from Myrtaceae family and characterization of their antibacterial activity. Mater Lett 209:201–206. https://www.academia.edu/34365618/CrossRef

22.

Patil MP, Singh RD, Koli PB, Patil KT, Jagdale BS, Tipare AR et al. (2018) Antibacterial potential of silver nanoparticles synthesized using Madhuca longifolia flower extract as a green resource. Microb Pathog 121:184–189. https://pubmed.ncbi.nlm.nih.gov/29807133/CrossRef

23.

Raj S, Chand Mali S, Trivedi R (2018) Green synthesis and characterization of silver nanoparticles using Enicostemma axillare (Lam.) leaf extract. Biochem Biophys Res Commun 503:2814–2819. https://pubmed.ncbi.nlm.nih.gov/30100057/CrossRef

24.

Rajakumar G, Gomathi T, Thiruvengadam M, Devi Rajeswari V, Kalpana VN, Chung IM (2017) Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract. Microb Pathog 103:123–128. https://pubmed.ncbi.nlm.nih.gov/28025099/CrossRef

25.

Sivaramakrishnan M, Sharavanan VJ et al. (2019) Green synthesized silver nanoparticles using aqueous leaf extracts of Leucas aspera exhibits antimicrobial and catalytic dye degradation properties. SN Appl Sci 1:208. https://doi.org/10.1007/s42452-019-0221-1CrossRef

26.

Elaloui M, Laamouri A(2016) Phytoconstituents of leaf extracts of Ziziphus jujuba Mill. plants harvested in Tunisia Ind Crops Prod 83:133–139. https://oatao.univ-toulouse.fr/25274/CrossRef

27.

Sankarganesh P, Ganesh Kumar A, Parthasarathy V, Joseph B, Priyadharsini N, Anbarasan R (2021) Synthesis of Murraya koenigii mediated silver nanoparticles and their invitro and invivo biological potential. J Inorg Organomet Polym Mater 31:2971–2979. https://doi.org/10.1007/s10904-021-01894-6CrossRef

28.

Zhang K, Liu X, Oliver S et al. (2019) Synthesis of silver nanoparticles (AgNPs) from leaf extract of Salvia miltiorrhiza and its anticancer potential in human prostate cancer LNCaP cell lines. Artif Cells Nanomed Biotechnol 47:2846–2854. https://doi.org/10.1080/21691401.2019.1638792CrossRef

29.

Uthaya Chandirika J, Annadurai G (2018) Biosynthesis and characterization of silver nanoparticles using leaf extract Abutilon indicum. Glob J Biotechnol Biochem 13:07–11. https://www.researchgate.net/publication/324694835

30.

Bahrami-Teimoori B, Nikparast Y, Hojatianfar M, Akhlaghi M, Ghorbani R, Pourianfar HR (2017) Characterisation and antifungal activity of silver nanoparticles biologically synthesised by amaranthus retroflexus leaf extract. J Exp Nanosci 12:129–139. https://doi.org/10.1080/17458080.2017.1279355CrossRef

31.

Garibo D, Borbón-Nuñez HA, de León JND, García Mendoza E, Estrada I, Toledano-Magaña Y et al. (2020) Green synthesis of silver nanoparticles using lysiloma acapulcensis exhibit high-antimicrobial activity. Sci Rep 10:12805–12811. https://www.nature.com/articles/s41598-020-69606-7CrossRef

32.

Susmila Aparna G, Venkata Subbaiah K (2021) Multifaceted phytogenic silver nanoparticles by an insectivorous plant Drosera spatulata Labill var. bakoensis and its potential therapeutic applications. Sci Rep 11:21969. https://www.nature.com/articles/s41598-021-01281-8CrossRef

33.

Ganesh Kumar A, Sankarganesh P, Parthasarathy V, Bhuvaneshwari J, Anbarasan R (2022) Invitro and invivo biological potential of the prepared Feroniella lucida mediated Silver nanoparticles. J Sol Gel Sci Technol 101:411–419. https://doi.org/10.1007/s10971-022-05728-wCrossRef

34.

Chokkareddy R, Redhi GG (2018) Green synthesis of metal nanoparticles and its reaction mechanisms, synthesis, characterization and their applications. Green Met Nanopart 84:113–139. https://doi.org/10.1002/9781119418900.ch4CrossRef

35.

Zhang Y, Vlahovic B (2016) A review on preparation and applications of silver-containing nanofibers. Nanoscale Res Lett 11:80. https://doi.org/10.1186/s11671-016-1286-zCrossRef

36.

Sharifi-Rad M, Pohl P, Epifano F (2021) Phytofabrication of silver nanoparticles (AgNPs) with pharmaceutical capabilities using Otostegia persica (Burm.) Boiss. leaf extract. Nanomaterials 11:1045. https://www.mdpi.com/2079-4991/11/4/1045CrossRef

37.

Susmila AG, Venkata Subbaiah K, Sai Gopal DVR, Subba Rao Y, Varada Reddy A (2014) Efficient and robust biofabrication of silver nanoparticles by cassia alata leaf extract and their antimicrobial activity. J Nanostructure Chem 4:1–9. https://doi.org/10.1007/s40097-014-0082-5CrossRef

38.

Abubakar I, Mann A, Mathew JT (2015) Phytochemical composition, antioxidant and anti-nutritional properties of root-bark and leaf methanol extracts of Senna alata L. grown in Nigeria. Afr J Pure Appl Chem 9(5):91–97. https://www.researchgate.net/publication/334647622CrossRef

39.

Kero Jemal BV, Sandeep Sudhakar P (2017) Synthesis, characterization, and evaluation of the antibacterial activity of Allophylus serratus leaf and leaf derived callus extracts mediated silver nanoparticles. J Nanomater 10:1–11. https://www.hindawi.com/journals/jnm/2017/4213275/CrossRef

40.

Annadurai G, Gnana Jobitha GS, Rajeshkumar S, Kannan C (2013) Preparation and characterization of fruit-mediated silver nanoparticles using pomegranate extract and assessment of its antimicrobial activities. J Environ Nanotechnol 2(1):04–10. https://www.researchgate.net/publication/236248752CrossRef

41.

Daizy P (2011) Mangifera indica leaf-assisted biosynthesis of well-dispersed silver nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc 78(1):327–331. https://pubmed.ncbi.nlm.nih.gov/21030295/CrossRef

42.

Vanaja M, Annadurai G (2013) Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Appl Nanosci 14:5087–5107. https://doi.org/10.1007/s13204-012-0121-9CrossRef

43.

Zaheer Z, Rafiuddin (2012) Silver nanoparticles to self-assembled films: green synthesis and characterization. Colloids Surf B Biointerfaces 90:48–52. https://pubmed.ncbi.nlm.nih.gov/22055624/CrossRef

44.

Jafarizada A, Safaee K, Gharibian S, Omidi Y, Ekinci D (2015) Biosynthesis and in-vitro study of gold nanoparticles using mentha and pelargonium extracts. Proc Mater Sci 11:224–230. https://www.sciencedirect.com/science/article/pii/S2211812815004551CrossRef

45.

Suresh AK, Doktycz MJ, Wang W, Moon JW, Meyer HM, Hensley DK, Allison DP, Phelps TJ, Pelletier DA (2011) Monodispersed biocompatible silver sulfide nanoparticles facile extracellular biosynthesis using the γ-proteobacterium, Shewanella oneidensis. Acta Biomater 7:4253–4258. https://pubmed.ncbi.nlm.nih.gov/21798382/CrossRef

46.

Rajeshkumar S, Bharath LV (2017) Mechanism of plant-mediated synthesis of silver nanoparticles—a review on biomolecules involved, characterization and antibacterial activity. Chem biol interact 273:219–227. https://pubmed.ncbi.nlm.nih.gov/28647323/CrossRef

47.

Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess 1:3. https://doi.org/10.1186/s40643-014-0003-yCrossRef

48.

Bawskar M, Deshmukh S, Bansod S, Gade A, Rai M (2015) Comparative analysis of biosynthesised and chemosynthesised silver nanoparticles with special reference to their antibacterial activity against pathogens. IET Nanobiotechnol 9(3):107–113CrossRef

49.

Juan L, Zhimin Z, Anchun M, Lei L, Jingchao Z (2010) Deposition of silver nanoparticles on titanium surface for antibacterial effect. Int J Nanomed 5:261–267. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865021/CrossRef

50.

Raju V, Ramar T, Krishnasamy M, Palani G, Krishnasamy K, Soundrapandian K (2012) Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cell lines. Process Biochem 47:2405–2410. https://www.researchgate.net/publication/234034427CrossRef

51.

Venugopal K, Rather HA, Rajagopal K, Shanthi MP, Sheriff K, Illiyas M, Rather RA, Manikandan E, Uvarajan S, Bhaskar M, Maaza M (2017) Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. J Photochem Photobiol B Biol 167:282–289. https://www.sciencedirect.com/science/article/abs/pii/S1011134416309125

52.

Bressan E, Ferroni L, Gardin C (2013) Silver nanoparticles and mitochondrial interaction. Nano Implant Dent 47:2405–2410. https://www.hindawi.com/journals/ijd/2013/312747/

53.

Saravanan A, Parthasarathy V, Senthil Kumar P (2022) Antimicrobial activity and cytotoxicity effect of the prepared bioactive Ag-NPs using Senna alata leaf extract on MCF-7 cancer cell line and brine shrimp. J Sol Gel Sci Technol 103:766CrossRef

54.

Sumitha S, Vasanthi S, Shalini S, Chinni SV, Gopinath SCB, Anbu P, Bahari MB, Harish R, Kathiresan S, Ravichandran V (2018) Phyto-mediated photo catalysed green synthesis of silver nanoparticles using Durio Zibethinus seed extract: antimicrobial and cytotoxic activity and photocatalytic applications. Molecules 23(12):3311–3327CrossRef

55.

Supicha K, Varanut W, Niwat K, Tawewan T, Wannee J (2018) Siver nanoparticles toxicity in brine shrimp and its histopathological analysis. Int J Nanosci 17(6):1850007. https://www.researchgate.net/publication/321727350CrossRef

Title: Synthesis and characterization of bioactive silver nanoparticles: evaluation of their anticancer potential against MCF7 cell line and antibacterial activity against foodborne pathogens
Authors: A. Saravanan
V. Parthasarathy
P. Senthil Kumar
Publication date: 25-10-2023
Publisher: Springer US
Published in: Journal of Sol-Gel Science and Technology / Issue 1/2024
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI: https://doi.org/10.1007/s10971-023-06243-2

Springer Professional

Abstract

Graphical abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Other articles of this Issue 1/2024

Synthesis of zeolitic imidazolate framework-67 aerogel by anion exchange

Electrical and low-field magnetoresistance transport effect of La0.7Ca0.3MnO3: MnO2 composite ceramics

Terahertz based optical & electrical properties of PZT

Incorporation of nylon-11 into poly (vinylidene fluoride) gel electrolytes membrane for high safety lithium-ion batteries

Effect of Gd3+ substitution on structural, morphological, and magnetic properties of BiFeO3 nanoparticles

Chitosan-boehmite reinforced AgNPs prepared by the sol-gel method for cost-effective water disinfection

Premium Partners