Top

Journal of Materials Science

Published in:

13-09-2019 | Materials for life sciences

Urease adsorption immobilization on ionic liquid-like macroporous polymeric support

Authors: Hongjin Kim, Fatima Hassouna, František Muzika, Merve Arabacı, Dušan Kopecký, Ivona Sedlářová, Miroslav Šoóš

Published in: Journal of Materials Science | Issue 24/2019

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

In this work, we report the synthesis and application of polymeric macroporous materials functionalized with ionic liquid (IL)-like moieties to serve as a support for enzyme immobilization. The method was based on bottom-up approach, where poly(styrene–divinylbenzene) (PS–DVB) nanoparticles were used as building blocks to form porous structures. Surface functionalization was done by introducing 1-butyl imidazole into the PS–DVB support to form IL-like imidazolium, which was consequently used for enzyme adsorption immobilization on the porous surface. To demonstrate activity of immobilized enzyme, hydrolysis of urea catalyzed by Jack bean urease was used as a model reaction. The enzymatic activity of the urease to convert urea solution into carbon dioxide (hydrocarbonates in water solution) and ammonia under acidic conditions were monitored both by measuring changes in pH and by a color change in cresol red pH indicator. The immobilized urease was able to provide hydrolysis of urea solution for 30 days while maintaining its activity over 100% compared to free enzyme solution. The activity of freshly immobilized enzyme was increased up to 285% compared to free urease solution under acidic conditions, which is caused by an acidic shift of activity–pH bell-shaped curve. Prepared porous material with immobilized urease was able to undergo four consequent cycles over the period of 4 days with only 15% decrease in activity. Overall, the results indicated that the polymeric support is well suitable to combine the advantages of macroporous material with IL-like surface moieties for enzyme immobilization and its consequent application in bio-catalytic reactions.

previous article Iron phosphides supported on three-dimensional iron foam as an efficient electrocatalyst for water splitting reactions

next article Ultra-long ZnO/carbon nanofiber as free-standing electrochemical sensor for dopamine in the presence of uric acid

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Available only for authorised users

Hentze H-P, Antonietti M (2008) Porous polymers and resins. Wiley-VCH Verlag GmbH, Weinheim

Okay O (2000) Macroporous copolymer networks. Prog Polym Sci 25:711–779CrossRef

Sherrington DC (1998) Preparation, structure and morphology of polymer supports. Chem Commun 21:2275–2286CrossRef

Lv Y, Lin Z, Tan T, Svec F (2014) Preparation of reusable bioreactors using reversible immobilization of enzyme on monolithic porous polymer support with attached gold nanoparticles. Biotechnol Bioeng 111:50–58CrossRef

Urban J, Svec F, Fréchet JMJ (2012) A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters. Biotechnol Bioeng 109:371–380CrossRef

Izquierdo DF, Yates M, Lozano P, Burguete MI, García-Verdugo E, Luis SV (2014) Macroporous polymers tailored as supports for large biomolecules: ionic liquids as porogenic solvents and as surface modifiers. React Funct Polym 85:20–27CrossRef

Garcia-Diego C, Cuellar J (2005) Synthesis of macroporous poly(styrene-co-divinylbenzene) microparticles using n-heptane as the porogen: quantitative effects of the DVB concentration and the monomeric fraction on their structural characteristics. Ind Eng Chem Res 44:8237–8247CrossRef

Afeyan NB, Fulton SP, Gordon NF, Mazsaroff I, Várady L, Regnier FE (1990) Perfusion chromatography: an approach to purifying biomolecules. Bio/Technology 8:203–206

Wang QC, Hosoya K, Svec F, Frechet JMJ (1992) Polymeric porogens used in the preparation of novel monodispersed macroporous polymeric separation media for high-performance liquid chromatography. Anal Chem 64:1232–1238CrossRef

10.

Perrier-Cornet R, Héroguez V, Thienpont A, Babot O, Toupance T (2008) Functional crosslinked polymer particles synthesized by precipitation polymerization for liquid chromatography. J Chromatogr A 1179:2–8CrossRef

11.

Svec F, Frechet JMJ (1996) New designs of macroporous polymers and supports: from separation to biocatalysis. Science 273:205–211CrossRef

12.

Luis SV, Garcia-Verdugo E (eds) (2009) Chemical reactions and processes under flow conditions. Royal Society of Chemistry, London

13.

Shih Y-H, Singco B, Liu W-L, Hsu C-H, Huang H-Y (2011) A rapid synthetic method for organic polymer-based monoliths in a room temperature ionic liquid medium via microwave-assisted vinylization and polymerization. Green Chem 13:296–299CrossRef

14.

Singco B, Lin C-L, Cheng Y-J, Shih Y-H, Huang H-Y (2012) Ionic liquids as porogens in the microwave-assisted synthesis of methacrylate monoliths for chromatographic application. Anal Chim Acta 746:123–133CrossRef

15.

Kubisa P (2009) Ionic liquids as solvents for polymerization processes—progress and challenges. Prog Polym Sci 34:1333–1347CrossRef

16.

Erdmenger T, Guerrero-Sanchez C, Vitz J, Hoogenboom R, Schubert US (2010) Recent developments in the utilization of green solvents in polymer chemistry. Chem Soc Rev 39:3317–3333CrossRef

17.

Tripathi AK, Singh RK (2018) Immobilization induced molecular compression of ionic liquid in ordered mesoporous matrix. J Phys D Appl Phys 51:075301CrossRef

18.

Tripathi AK, Verma YL, Shalu, Singh VK, Balo L, Gupta H, Singh SK, Singh VK (2017) Quasi solid-state electrolytes based on ionic liquid (IL) and ordered mesoporous matrix MCM-41 for supercapacitor application. J Solid State Electrochem 21:3365–3371CrossRef

19.

Plechkova NV, Seddon KR (2008) Applications of ionic liquids in the chemical industry. Chem Soc Rev 37:123–150CrossRef

20.

Tripathi AK, Singh RK (2018) Development of ionic liquid and lithium salt immobilized MCM-41 quasi solid–liquid electrolytes for lithium batteries. J Energy Storage 15:283–291CrossRef

21.

Kubisa P (2004) Application of ionic liquids as solvents for polymerization processes. Prog Polym Sci 29:3–12CrossRef

22.

Marti N, Quattrini F, Butte A, Morbidelli M (2005) Production of polymeric materials with controlled pore structure: the ‘‘Reactive gelation’’ process. Macromol Mater Eng 290:221–229CrossRef

23.

Lamprou A, Köse I, Peña Aguirre Z, Storti G, Morbidelli M, Soos M (2014) Macroporous polymer particles via reactive gelation under shear: effect of primary particle properties and operating parameters. Langmuir 30:13970–13978CrossRef

24.

Lamprou A, Köse I, Storti G, Morbidelli M, Soos M (2014) Synthesis of macroporous polymer particles using reactive gelation under shear. Langmuir 30:6946–6953CrossRef

25.

Meakin P (1988) Fractal aggregates. Adv Colloid Interface Sci 28:249–331CrossRef

26.

Brand B, Morbidelli M, Soos M (2015) Shear-induced reactive gelation. Langmuir 31:12727–12735CrossRef

27.

Bechtle M, Butte A, Storti G, Morbidelli M (2010) Preparation of macroporous methacrylate-based monoliths for chromatographic applications by the reactive gelation process. J Chromatogr A 1217:4675–4681CrossRef

28.

de Neuville BC, Lamprou A, Morbidelli M, Soos M (2014) Perfusive ion-exchange chromatographic materials with high capacity. J Chromatogr A 1374:180–188CrossRef

29.

Lamprou A, Gavriilidou A-F-M, Storti G, Soos M, Morbidelli M (2015) Application of polymeric macroporous supports for temperature-responsive chromatography of pharmaceuticals. J Chromatogr A 1407:90–99CrossRef

30.

Cui S, Zhou Q-W, Wang X-L et al (2017) Immobilization of lipase onto N-succinyl-chitosan beads and its application in the enrichment of polyunsaturated fatty acids in fish oil. J Food Biochem 41:e12395CrossRef

31.

Rath P, Tripathi P, Kayastha AM (2012) Immobilization of α-amylase from germinated mung beans (Vigna radiata) on Fuller’s earth by adsorption. J Plant Biochem Biotechnol 21:229–234CrossRef

32.

Lassouane F, Aït-Amar H, Amrani S, Rodriguez-Couto S (2019) A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions. Bioresour Technol 271:360–367CrossRef

33.

Rojas-Mercado AS, Moreno-Cortez IE, Lucio-Porto R, Pavón LL (2018) Encapsulation and immobilization of ficin extract in electrospun polymeric nanofibers. Int J Biol Macromol 118:2287–2295CrossRef

34.

Baysal Z, Aksoy E, Dolak İ, Ersöz A, Say R (2018) Adsorption behaviours of lysozyme onto poly-hydroxyethyl methacrylate cryogels containing methacryloyl antipyrine-Ce(III). Int J Polym Mater Polym Biomater 67:199–204CrossRef

35.

Zhao K, Cao X, Di Q et al (2017) Synthesis, characterization and optimization of a two-step immobilized lipase. Renew Energy 103:383–387CrossRef

36.

Weiss WF, Hodgdon TK, Kaler EW, Lenhoff AM, Roberts CJ (2007) Nonnative protein polymers: structure, morphology, and relation to nucleation and growth. Biophys J 93:4392–4403CrossRef

37.

Grancic P, Illeova V, Polakovic M, Sefcik J (2012) Thermally induced inactivation and aggregation of urease: experiments and population balance modelling. Chem Eng Sci 70:14–21CrossRef

38.

Cui J, Sun B, Lin T, Feng Y, Jia S (2018) Enzyme shielding by mesoporous organosilica shell on Fe₃O₄@silica yolk–shell nanospheres. Int J Biol Macromol 117:673–682CrossRef

39.

Pogorilyi RP, Goncharik VP, Kozhara LI, Zub YL (2008) Covalent immobilization of urease on polysiloxane matrices containing 3-aminopropyl and 3-mercaptopropyl groups. Appl Biochem Microbiol 44:561–565CrossRef

40.

Zambelli B, Musiani F, Benini S, Ciurli S (2011) Chemistry of Ni²⁺ in urease: sensing, trafficking, and catalysis. Acc Chem Res 44:520–530CrossRef

41.

Wang S, Lee MH, Hausinger RP, Clark PA, Wilcox DE, Scott RA (1994) Structure of the dinuclear active site of urease. X-ray absorption spectroscopic study of native and 2-mercaptoethanol-inhibited bacterial and plant enzymes. Inorg Chem 33:1589–1593CrossRef

42.

Nielsen JE, McCammon JA (2003) Calculating pKa values in enzyme active sites. Protein Sci Publ Protein Soc 12:1894–1901CrossRef

43.

Yang D, Fan J, Cao F, Deng Z, Pojman JA, Ji L (2019) Immobilization adjusted clock reaction in the urea–urease–H⁺ reaction system. RSC Adv 9:3514–3519CrossRef

44.

Hu G, Pojman JA, Scott SK, Wrobel MM, Taylor AF (2010) Base-catalyzed feedback in the urea–urease reaction. J Phys Chem B 114:14059–14063CrossRef

45.

Bubanja IN, Bánsági T, Taylor AF (2018) Kinetics of the urea–urease clock reaction with urease immobilized in hydrogel beads. React Kinet Mech Catal 123:177–185CrossRef

46.

Hoare JP, Laidler KJ (1950) The molecular kinetics of the urea–urease system. II. The inhibition by products. J Am Chem Soc 72:2487–2489CrossRef

47.

Marzadori C, Miletti S, Gessa C, Ciurli S (1998) Immobilization of jack bean urease on hydroxyapatite: urease immobilization in alkaline soils. Soil Biol Biochem 30:1485–1490CrossRef

48.

Zhou J, Cao J, Huang W et al (2013) Preparation and property of urease immobilization with cationic poly(4-vinylpyridine) functionalized colloidal particles. Chem Biochem Eng Q 27:431–437

49.

de Brito AK, Nordi CSF, Caseli L (2015) Algal polysaccharides as matrices for the immobilization of urease in lipid ultrathin films studied with tensiometry and vibrational spectroscopy: physical–chemical properties and implications in the enzyme activity. Colloids Surf B Biointerfaces 135:639–645CrossRef

50.

Kirdeciler SK, Soy E, Öztürk S et al (2011) A novel urea conductometric biosensor based on zeolite immobilized urease. Talanta 85:1435–1441CrossRef

51.

Jia W, Wang B, Wang C, Sun H (2017) Tourmaline and biochar for the remediation of acid soil polluted with heavy metals. J Environ Chem Eng 5:2107–2114CrossRef

52.

Ispirli Doğaç Y, Deveci İ, Teke M, Mercimek B (2014) TiO₂ beads and TiO₂–chitosan beads for urease immobilization. Mater Sci Eng, C 42:429–435CrossRef

53.

Somturk B, Yilmaz I, Altinkaynak C, Karatepe A, Özdemir N, Ocsoy I (2016) Synthesis of urease hybrid nanoflowers and their enhanced catalytic properties. Enzyme Microb Technol 86:134–142CrossRef

54.

Tang X, Liu S, Wang S, Zhang Q, Cheng Z (2014) Preparation of reversibly immobilized Jack bean urease on microchannel surface and application for enzyme inhibition assay. Microfluid Nanofluid 17:721–728CrossRef

55.

Srivastava PK, Kayastha AM, Srinivasan (2001) Characterization of gelatin-immobilized pigeonpea urease and preparation of a new urea biosensor. Biotechnol Appl Biochem 34:55–62CrossRef

56.

Singh AK, Singh M, Verma N (2017) Extraction, purification, kinetic characterization and immobilization of urease from Bacillus sphaericus MTCC 5100. Biocatal Agric Biotechnol 12:341–347CrossRef

57.

de Melo JV, Cosnier S, Mousty C, Martelet C, Jaffrezic-Renault N (2002) Urea biosensors based on immobilization of urease into two oppositely charged clays (laponite and Zn–Al layered double hydroxides). Anal Chem 74:4037–4043CrossRef

58.

Syu M-J, Chang Y-S (2009) Ionic effect investigation of a potentiometric sensor for urea and surface morphology observation of entrapped urease/polypyrrole matrix. Biosens Bioelectron 24:2671–2677CrossRef

59.

Agelis G, Resvani A, Durdagi S et al (2012) The discovery of new potent non-peptide Angiotensin II AT1 receptor blockers: a concise synthesis, molecular docking studies and biological evaluation of N-substituted 5-butylimidazole derivatives. Eur J Med Chem 55:358–374CrossRef

60.

Blesic M, Nimal Gunaratne HQ, Nockemann P, McCarron P, Seddon KR (2013) Controlled fragrance delivery in functionalised ionic liquid–enzyme systems. RSC Adv 3:329–333CrossRef

61.

Soos M, Lattuada M, Sefcik J, Morbidelli M (2009) Interpretation of light scattering and turbidity measurements in aggregated systems: effect of intra-cluster multiple-light scattering. J Phys Chem B 113:14962–14970CrossRef

62.

Kerker M (1969) The scattering of light. Academic Press, New York

63.

Bohren DR, Huffman CF (1983) Absorption and scattering of light by small particles. Wiley-Interscience, New York

64.

Sorensen CM (2001) Light scattering by fractal aggregates: a review. Aerosol Sci Technol 35:648–687CrossRef

65.

Lin M, Lindsay HM, Weitz DA, Klein R, Ball RC, Meakin P (1990) Universal reaction-limited aggregation. Phys Rev A 41:2005–2020CrossRef

66.

Sandkühler P, Lattuada M, Wu H, Sefcik J, Morbidelli M (2005) Further insights into the universality of colloidal aggregation. Adv Colloid Interface Sci 113:65–83CrossRef

67.

Krajewska B, Ciurli S (2005) Jack bean (Canavalia ensiformis) urease. probing acid–base groups of the active site by pH variation. Plant Physiol Biochem 43:651–658CrossRef

Title: Urease adsorption immobilization on ionic liquid-like macroporous polymeric support
Authors: Hongjin Kim
Fatima Hassouna
František Muzika
Merve Arabacı
Dušan Kopecký
Ivona Sedlářová
Miroslav Šoóš
Publication date: 13-09-2019
Publisher: Springer US
Published in: Journal of Materials Science / Issue 24/2019
Print ISSN: 0022-2461
Electronic ISSN: 1573-4803
DOI: https://doi.org/10.1007/s10853-019-03980-0

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 24/2019

Superhydrophobicity and anti-icing of CF/PEEK composite surface with hierarchy structure

Strength of transparent ceramic composites with spinel

High-throughput-generating water-in-water droplet for monodisperse biocompatible particle synthesis

Humidity sensing behavior and its influence on the dielectric properties of (In + Nb) co-doped TiO2 ceramics

Improvement in alkali metal resistance of commercial V2O5–WO3/TiO2 SCR catalysts modified by Ce and Cu

Formation mechanism of nanoscale transformation twinning in ultra-low-carbon high-strength pipeline steels

Premium Partners