A new Cu(II)-based coordination polymer: application values on liver cancer through down-regulating relative expression of miRNA9

In current research, a novel coordination polymer based on Cu(II) having a 1D spiro-like chain architecture has been created with the reaction between HL (4-(4-(2-chloroimidazo[1,2-a]pyridin-3-yl)-[2,3′-bipyridin]-6-yl)benzoic acid), the custom designed organic ligand and Cu(NO₃)₂·3H₂O under the reaction conditions of solvothermal, and its chemical formula is [Cu(L)₂]·2DMF·3H₂O (1). At first, the real-time RT-PCR was accomplished for the determination of the miRNA9 expression level in normal tissue and liver cancer tissue. And the compound’s influence on the miRNA9 relative expression in the cancer cells was next investigated as well. The western blotting assay was in-depth employed for the determination of the PDGF signaling pathway expression in the liver cancer cells after treated with the new compound. In the end, we investigate the direct regulation of miRNA9 on signaling pathway of PDGF by luciferase reporter gene analysis and bioinformatics analysis. Suggested by the simulation of molecular docking, it is found that the carboxyl group of Cu complex could bind to probe protein, at the same time, the pyridine ring also could bind to the probe protein, and therefore suggested that the Cu complex exhibited excellent biological activities.