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Biomass Conversion and Biorefinery

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02-12-2017 | Original Article

A quick and effective method for evaluating substrate-enzyme systems in the enzymatic hydrolysis of lignocellulosic biomass

Authors: Javier Ulises Hernández-Beltrán, Karla Cervantes-Quintero, Héctor Hernández-Escoto

Published in: Biomass Conversion and Biorefinery | Issue 2/2018

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Abstract

This work describes a microreaction technique to carry out hydrolysis of certain substrate by certain enzyme under several conditions, and reports its usefulness to determine effective process conditions and to compare performances between different systems substrate-enzyme, in a quick and economic way. The microreaction system consists of microtubes, as microreactors, that are placed into a thermomixer, allowing a set of 24 experiments at a certain constant temperature but with different pH and substrate-enzyme ratios. The outcomes of the numerous experiments are processed via response surface methodology and means analysis. In order to illustrate the technique effectiveness, it is discussed the comparison of two commercial enzyme complexes, and two delignification procedures of wheat straw.

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Title: A quick and effective method for evaluating substrate-enzyme systems in the enzymatic hydrolysis of lignocellulosic biomass
Authors: Javier Ulises Hernández-Beltrán
Karla Cervantes-Quintero
Héctor Hernández-Escoto
Publication date: 02-12-2017
Publisher: Springer Berlin Heidelberg
Published in: Biomass Conversion and Biorefinery / Issue 2/2018
Print ISSN: 2190-6815
Electronic ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-017-0297-z

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