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Colloid and Polymer Science

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25-05-2018 | Original Contribution

Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC

Authors: Sergey K. Filippov, Aristeidis Papagiannopoulos, Anna Riabtseva, Stergios Pispas

Published in: Colloid and Polymer Science | Issue 7/2018

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Abstract

The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.

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Lynch I, Lundqvist M, Stigler J, Elia G, Cedervall T, Dawson KA (2008) Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proc Natl Acad Sci U S A 105:14265–14270CrossRefPubMedPubMedCentral

Saptarshi SR, Duschl A, Lopata AL (2013) Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. J Nanobiotechnol 11:26CrossRef

Wiseman T, Williston S, Brandts JF, Lin L-N (1989) Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal Biochem 179:131–137CrossRefPubMed

O’Brien R, Ladbury JE, Chowdry BZ (2001) Isothermal titration calorimetry of biomolecules. In: Harding SE, Chowdry BZ (eds) Protein-ligand interactions: hydrodynamics and calorimetry. Oxford University Press, Oxford, pp 263–286

Leavitt S, Freire E (2001) Direct measurement of protein binding energetics by isothermal titration calorimetry. Curr Opin Struct Biol 11:560–566CrossRefPubMed

Roselin LS, Lin M-S, Lin P-H, Chang Y, Chen W-Y (2010) Recent trends and some applications of isothermal titration calorimetry in biotechnology. Biotechnol J 5:85–98CrossRefPubMed

Albarkah YA, Green RJ, Khutoryanskiy VV (2015) Probing the mucoadhesive interactions between porcine gastric mucin and some water-soluble polymers. Macromol Biosci 15:1546–1553CrossRefPubMed

Riabtseva A, Kaberov LI, Kučka J, Bogomolova A, Stepanek P, Filippov SK, Hruby M (2017) Polyelectrolyte pH-responsive protein-containing nanoparticles: the physicochemical supramolecular approach. Langmuir 33:764–772CrossRefPubMed

Lindman S, Lynch I, Thulin E, Nilsson H, Dawson KA, Linse S (2007) Systematic investigation of the thermodynamics of HSA adsorption to N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles. Effects of particle size and hydrophobicity. Nano Lett 7:914–920CrossRefPubMed

10.

Shubhra QTH, Tóth J, Gyenis J, Feczkó T (2014) Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption. Coll Surf B: Biointerf 122:529–536CrossRef

11.

Mertens HDT, Svergun DI (2010) Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J Struct Biol 172:128–141CrossRefPubMed

12.

Semisotnov GV, Kihara H, Kotova NV, Kimura K, Amemiya Y, Wakabayashi K, Serdyuk IN, Timchenko AA, Chiba K, Nikaido K, Ikura T, Kuwajima K (1996) Protein globularization during folding. A study by synchrotron small-angle X-ray scattering. J Mol Biol 262:559–574CrossRefPubMed

13.

Carl N, Prévost S, Fitzgerald JPS, Karg M (2017) Salt-induced cluster formation of gold nanoparticles followed by stopped-flow SAXS, DLS and extinction spectroscopy. Phys Chem Chem Phys 19:16348–16357CrossRefPubMed

14.

Fürst C, Zhang P, Roth SV, Drechsler M, Förster S (2016) Self-assembly of block copolymers via micellar intermediate states into vesicles on time scales from milliseconds to days. Polymer 107:434–444CrossRef

15.

Henzler K, Rosenfeldt S, Wittemann A, Harnau L, Finet S, Narayanan T, Ballauff M (2008) Directed motion of proteins along tethered polyelectrolytes. Phys Rev Lett 100:158301e4CrossRef

16.

Henzler K, Haupt B, Rosenfeldt S, Harnau L, Narayanan T, Ballauff M (2011) Interaction strength between proteins and polyelectrolyte brushes: a small angle X-ray scattering study. Phys Chem Chem Phys 13:17599–17605CrossRefPubMed

17.

Wang W, Li L, Henzler K, Lu Y, Wang J, Han H, Tian Y, Wang Y, Zhou Z, Lotze G, Narayanan T, Ballauff M, Guo X (2017) Protein immobilization onto cationic spherical polyelectrolyte brushes studied by small angle X-ray scattering. Biomacromolecules 18:1574–1581CrossRefPubMed

18.

Segel DJ, Bachmann A, Hofrichter J, Hodgson KO, Doniach S, Kiefhaber T (1999) Characterization of transient intermediates in lysozyme folding with time-resolved small-angle X-ray scattering. J Mol Biol 288:489–499CrossRefPubMed

19.

Papagiannopoulos A, Meristoudi A, Hong K, Pispas S (2016) Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme. Polymer 83:111–115CrossRef

20.

Papagiannopoulos A, Meristoudi A, Pispas S, Radulescu A (2016) Micelles from HOOC-PnBA-b-PAA-C12H15 diblock amphiphilic polyelectrolytes as protein nanocarriers. Biomacromolecules 17:3816–3827CrossRefPubMed

21.

Papagiannopoulos A, Meristoudi A, Pispas S, Keiderling U (2016) Thermoresponsive behavior of micellar aggregates from end-functionalized PnBA-b-PNIPAM-COOH block copolymers and their complexes with lysozyme. Soft Matter 12:6547–6556CrossRefPubMed

22.

Papagiannopoulos A, Zhao J, Zhang G, Pispas S, Radulescu A (2013) Thermoresponsive transition of a PEO-b-PNIPAM copolymer: from hierarchical aggregates to well defined ellipsoidal vesicles. Polymer 54:6373–6380CrossRef

23.

Papagiannopoulos A, Zhao J, Zhang G, Pispas S, Radulescu A (2014) Thermoresponsive aggregation of PS–PNIPAM–PS triblock copolymer: a combined study of light scattering and small angle neutron scattering. Eur Polym J 56:59–68CrossRef

24.

Blanchet CE, Spilotros A, Schwemmer F, Graewert MA, Kikhney A, Jeffries CM, Franke D, Mark D, Zengerle R, Cipriani F, Fiedler S, Roessle M, Svergun DI (2015) Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY). J Appl Crystallogr 48:431–443CrossRefPubMedPubMedCentral

25.

Konarev PV, Volkov VV, Sokolova AV, Koch MHJ, Svergun DI (2003) PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J Appl Crystallogr 36:1277–1282CrossRef

26.

Jakes J (1988) Testing of the constrained regularization method of inverting Laplace transform on simulated very wide quasielastic light scattering autocorrelation functions. Czechoslov J Phys 38:1305–1316CrossRef

27.

Stepanek P (1993) Data analysis in dynamic light scattering. In: Brown W (ed) Dynamic light scattering. The method and some applications. Clarendon Press, Oxford, pp 175–241

28.

Klíčová L, Šebej P, Štacko P, Filippov S, Bogomolova A, Klán P (2012) CTAB/water/chloroform reverse micelles: a closed or open association model? Langmuir 28:15185–15192CrossRefPubMed

29.

Angelov B, Angelova A, Filippov SK, Drechsler M, Štěpánek P, Lesieur S (2014) Multicompartment lipid cubic nanoparticles with high protein upload: millisecond dynamics of formation. ACS Nano 8:5216–5226CrossRefPubMed

30.

Angelov B, Angelova A, Filippov SK, Narayanan T, Drechsler M, Nicolas V, Štěpánek P, Couvreur P, Lesieur S (2013) DNA/fusogenic lipid nanocarrier assembly: millisecond structural dynamics. J Phys Chem Let 4:1959–1964CrossRef

31.

Filippov SK, Franklin JM, Konarev PV, Chytil P, Etrych T, Bogomolova A, Dyakonova M, Papadakis C, Radulescu A, Ulbrich K, Stepanek P, Svergun DI (2013) SAXS/SANS kinetic study of hydrolytically degradable polymer micelles intended for controlled drug delivery. Biomacromolecules 14:4061–4070CrossRefPubMed

Title: Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC
Authors: Sergey K. Filippov
Aristeidis Papagiannopoulos
Anna Riabtseva
Stergios Pispas
Publication date: 25-05-2018
Publisher: Springer Berlin Heidelberg
Published in: Colloid and Polymer Science / Issue 7/2018
Print ISSN: 0303-402X
Electronic ISSN: 1435-1536
DOI: https://doi.org/10.1007/s00396-018-4329-4

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