Top

Journal of Nanoparticle Research

Published in:

01-11-2014 | Research Paper

Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress

Authors: Qun Zhang, Kun Ge, Jianlei Duan, Shizhu Chen, Ran Zhang, Cuimiao Zhang, Shuxiang Wang, Jinchao Zhang

Published in: Journal of Nanoparticle Research | Issue 11/2014

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Cerium oxide nanoparticles (nanoceria) have been widely used in industries and biomedical fields due to its unique properties. Previous biodistribution studies of nanoceria in vivo have shown that they are accumulated in the bone of mice after intravenous administration, about 20 % of the total intake, however, the potential effect and the mechanism of nanoceria on bone metabolism are not well-understood. Our results showed that both 25 and 50 nm nanceria decreased the damage of cell viability induced by H₂O₂ in a dose-dependent manner. The apoptosis ratio of pre-incubated group with nanoceria was lower than the H₂O₂ group. The cellular uptake studies indicated that there was a dose-dependent accumulation of both two size nanoparticles in bone marrow stromal cells. Nanoceria could be uptaken by cells due to the synergistic effect of multiple endocytosis mechanisms, and then evenly distributed in the cytoplasm without entering the nucleus. Our results suggest that nanoceria could reduce intracellular ROS level induced by H₂O₂ in a dose-dependent manner, moreover, maintain the normal function of mitochondria, suggesting nanoceria may have potent applications for preventing or treating osteoporosis.

previous article Dispersions of geometric TiO2 nanomaterials and their toxicity to RPMI 2650 nasal epithelial cells

next article Continuous-flow biosynthesis of Au–Ag bimetallic nanoparticles in a microreactor

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Amin KA, Hassan MS, Awad el ST, Hashemel KS (2011) The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline. Int J Nanomed 6:143–149CrossRef

Bai XC, Lu D, Bai J, Zheng H, Ke ZY, Li XM, Luo SQ (2004) Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB. Biochem Biophys Res Commun 314:197–207CrossRef

Chen Q, Takeyama N, Brady G, Watson AJ, Dive C (1998) Blood cells with reduced mitochondrial membrane potential and cytosolic cytochrome c can survive and maintain clonogenity given appropriate signals to suppress apoptosis. Blood 92:4545–4553

Chen J, Patil S, Seal S, McGinnis JF (2006) Rare earth nanoparticles prevent retinal degeneration induced by intracellular peroxides. Nat Nanotechnol 1:142–150CrossRef

Colon J, Herrera L, Smith J, Patil S, Komanski C, Kupelian P, Seal S, Jenkins DW, Baker CH (2009) Protection from radiation-induced pneumonitis using cerium oxide nanoparticles. Nanomed Nanotechnol Biol Med 5:225–231

Corma A, Atienzar P, García H, Chane-Ching JY (2004) Hierarchically mesostructured doped cerium oxide with potential for solar-cell use. Nat Mater 3:394–397CrossRef

Das M, Patil S, Bhargava N, Kang JF, Riedel LM, Seal S, Hickman JJ (2007) Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons. Biomaterials 28:1918–1925CrossRef

Dong XT, Hong GY, Yu DC, Yu DS (1997) Synthesis and properties of cerium oxide nanometer powder by pyrolysis of amorphous citrate. J Mater Sci Technol 13:113–116

Estevez AY, Pritchard S, Harper K, Aston JW, Lynch A, Lucky JJ, Ludington JS, Chatani P, Mosenthal WP, Leiter JC, Andreescu S, Erlichman JS (2011) Neuroprotective mechanisms of cerium oxide nanoparticles in a mouse hippocampal brain slice model of ischemia. Free Radic Biol Med 51:1155–1163CrossRef

Greulich C, Diendorf J, Simon T, Eggeler G, Epple M, Köller M (2011) Uptake and intracellular distribution of silver nanoparticles in human mesenchymal stem cells. Acta Biomater 7:347–354CrossRef

Heckert EG, Karakoti AS, Seal S, Self WT (2008) The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials 29:2705–2709CrossRef

Hirst SM, Karakoti AS, Tyler RD, Sriranganathan N, Seal S, Reilly CM (2009) Anti-inflammatory properties of cerium oxide nanoparticles. Small 5:2848–2856CrossRef

Horie M, Nishio K, Fujita K, Endoh S, Miyauchi A, Saito Y, Iwahashi H, Yamamoto K, Murayama H, Nakano H, Nanashima N, Niki E, Yoshida Y (2009) Protein adsorption of ultrafine metal oxide and its influence on cytotoxicity toward cultured cells. Chem Res Toxicol 22:543–553CrossRef

Karakoti A, Hench LL, Seal S (2006) The potential toxicity of nanomaterials-The role of surfaces. JOM 58:77–82CrossRef

Karakoti A, Singh S, Dowding JM, Seal S, Self WT (2010) Redox-active radical scavenging nanomaterials. Chem Soc Rev 39:4422–4432CrossRef

Korsvik C, Patil S, Seal S, Self WT (2007) Superoxide dismutase mimetic properties exhibited by vacancy engineered ceria nanoparticles. Chem Commun 14:1056–1058CrossRef

Li XD, Wang JS, Chang B, Chen B, Guo C, Hou GQ, Huang DY, Du SX (2011) Contents lists available at sciencedirect journal of ethnopharmacology panax notoginseng saponins promotes proliferation and osteogenic differentiation of rat bone marrow stromal cells. J Ethnopharmacol 134:268–274CrossRef

Lin W, Huang YW, Zhou XD, Ma Y (2006) Toxicity of cerium oxide nanoparticles in human lung cancer cells. Int J Toxicol 25:451–457CrossRef

Liu AL, Zhang ZM, Zhu BF, Liao ZH, Liu Z (2004) Metallothionein protects bone marrow stromal cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation. Cell Biol Int 28:905–911CrossRef

Liu Y, Jiao F, Qiu Y, Li W, Lao F, Zhou G, Sun B, Xing G, Dong J, Zhao Y, Chai Z, Chen C (2009) The effect of Gd@C82(OH)22 nanoparticles on the release of Th1/Th2cytokines and induction of TNF-a mediated cellular immunity. Biomaterials 30:3934–3945CrossRef

Lord MS, Jung M, Teoh WY, Gunawan C, Vassie JA, Amal R, Whitelock JM (2012) Cellular uptake and reactive oxygen species modulation of cerium oxide nanoparticles in human monocyte cell line U937. Biomaterials 33:7915–7924CrossRef

Mancini M, Anderson BO, Caldwell E, Sedghinasab M, Paty PB, Hockenbery DM (1997) Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line. J Cell Biol 138:449–469CrossRef

Martinou I, Desagher S, Eskes R, Antonsson B, André E, Fakan S, Martinou JC (1999) The release of cytochrome c from mitochondria during apoptosis of NGF-deprived sympathetic neurons is a reversible event. J Cell Biol 144:883–889CrossRef

Matés JM, Sánchez-Jiménez FM (2000) Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell Biol 32:157–170CrossRef

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRef

Ozgocmen S, Kaya H, Fadillioglu E, Aydogan R, Yilmaz Z (2007) Role of antioxidant systems, lipid peroxidation and nitric oxide in postmenopausal osteoporosis. Mol Cell Biochem 295:45–52CrossRef

Pagliari F, Mandoli C, Forte G, Magnani E, Pagliari S, Nardone G, Licoccia S, Minieri M, Di Nardo P, Traversa E (2012) Cerium oxide nanoparticles protect cardiac progenitor cells from oxidative stress francesca pagliari. ACS Nano 6:3767–3775CrossRef

Ray G, Husain SA (2002) Oxidants, antioxidants and carcinogenesis. Indian J Exp Bio 40:1213–1232

Robert AY, Tu CA, Robert MP (2012) Distribution, elimination, and biopersistence to 90 days of a systemically introduced 30 nm ceria-engineered nanomaterial in rats. Toxicol Sci 127:256–268CrossRef

Sánchez-Rodríguez MA, Ruiz-Ramos M, Correa-Muñoz E, Mendoza-Núñez VM (2007) Oxidative stress as a risk factor for osteoporosis in elderly Mexicans as characterised by antioxidant enzymes. BMC Musculoskelet Disord 8:124–130CrossRef

Schubert D, Dargusch R, Raitano J, Chan SW (2006) Cerium and yttrium oxide nanoparticles are neuroprotective. Biochem Biophys Res Commun 342:86–91CrossRef

Singh S, Kumar A, Karakoti A, Seal S, Self WT (2010) Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles. Mol BioSyst 6:1813–1820CrossRef

Tarnuzzer RW, Colon J, Patil S, Seal S (2005) Vacancy engineered ceria nanostructures for protection from radiation-induced cellular damage. Nano Lett 5:2573–2577CrossRef

van Engeland M, Nieland LJ, Ramaekers FC, Schutte B, Reutelingsperger CP (1998) Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31:1–9CrossRef

Vander Heiden MG, Chandel NS, Williamson EK, Schumacker PT, Thompson CB (1997) Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria. Cell 91:627–637CrossRef

Verma S, Rajaratnam JH, Denton J, Hoyland JA, Byers RJ (2002) Adipoctyic proportion of bone marrow is inversely related to bone formation in osteoporosis. J Clin Pathol 55:693–698CrossRef

Wauquier F, Leotoing L, Coxam V, Guicheux J, Wittrant Y (2009) Oxidative stress in bone remodelling and disease. Trends Mol Med 15:468–477CrossRef

Xia T, Kovochich M, Liong M, Mädler L, Gilbert B, Shi H, Yeh JI, Zink JI, Nel AE (2008) Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano 2:2121–2134CrossRef

Yalin S, Bagis S, Polat G, Dogruer N, Cenk Aksit S, Hatungil R, Erdogan C (2005) Is there a role of free oxygen radicals in primary male osteoporosis? Clin Exp Rheumatol 23:689–692

Zhai YQ, Zhang SY, Pang H (2007) Preparation, characterization and photocatalytic activity of cerium oxide nanocrystalline using ammonium bicarbonate as precipitan. Mater Lett 61:1863–1866CrossRef

Zhao WH, Gou BD, Zhang TL, Wang K (2012) Lanthanum chloride bidirectionally influences calcification in bovine vascular smooth muscle cells. J Cell Biochem 113:1776–1786

Zhuang J, Dinsdale D, Cohen GM (1998) Apoptosis, in human monocytic THP.1 cells, results in the release of cytochrome c from mitochondria prior to their ultracondensation, formation of outer membrane discontinuities and reduction in inner membrane potential. Cell Death Differ 5:953–962CrossRef

Title: Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress
Authors: Qun Zhang
Kun Ge
Jianlei Duan
Shizhu Chen
Ran Zhang
Cuimiao Zhang
Shuxiang Wang
Jinchao Zhang
Publication date: 01-11-2014
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 11/2014
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2697-3

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 11/2014

Scalable preparation and characterization of GaN nanopowders with high crystallinity by soluble salts-assisted route

Biogenesis of TiO2 nanoparticles using endophytic Bacillus cereus

Singlet oxygen-generating from fluorescence probes based on denatured bovine serum albumin-conjugated CdTe quantum dots and photosensitizer Chlorin e6

Solution-processed anchoring zinc oxide quantum dots on covalently modified graphene oxide

Potential foliar fertilizers with copper and zinc dual micronutrients in nanocrystal suspension

Preparation and characterization of ZnO/graphene nanocomposite for improved photovoltaic performance

Premium Partners