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01-04-2014 | Original Paper

Controlled incorporation of deuterium into bacterial cellulose

Authors: Junhong He, Sai Venkatesh Pingali, Shishir P. S. Chundawat, Angela Pack, A. Daniel Jones, Paul Langan, Brian H. Davison, Volker Urban, Barbara Evans, Hugh O’Neill

Published in: Cellulose | Issue 2/2014

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Abstract

Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R _min = 1,132 ± 6 and 1,154 ± 3 Å and disk thickness, T = 128 ± 1 and 83 ± 1 Å for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.

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Title: Controlled incorporation of deuterium into bacterial cellulose
Authors: Junhong He
Sai Venkatesh Pingali
Shishir P. S. Chundawat
Angela Pack
A. Daniel Jones
Paul Langan
Brian H. Davison
Volker Urban
Barbara Evans
Hugh O’Neill
Publication date: 01-04-2014
Publisher: Springer Netherlands
Published in: Cellulose / Issue 2/2014
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-013-0067-4

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