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Journal of Nanoparticle Research

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01-10-2012 | Research Paper

Effect of surface charge on the cellular uptake of fluorescent magnetic nanoparticles

Authors: Slavko Kralj, Matija Rojnik, Rok Romih, Marko Jagodič, Janko Kos, Darko Makovec

Published in: Journal of Nanoparticle Research | Issue 10/2012

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Abstract

We report on the nanoparticle uptake into MCF10A neoT and PC-3 cells using flow cytometry, confocal microscopy, SQUID magnetometry, and transmission electron microscopy. The aim was to evaluate the influence of the nanoparticles’ surface charge on the uptake efficiency. The surface of the superparamagnetic, silica-coated, maghemite nanoparticles was modified using amino functionalization for the positive surface charge (CNPs), and carboxyl functionalization for the negative surface charge (ANPs). The CNPs and ANPs exhibited no significant cytotoxicity in concentrations up to 500 μg/cm³ in 24 h. The CNPs, bound to a plasma membrane, were intensely phagocytosed, while the ANPs entered cells through fluid-phase endocytosis in a lower internalization degree. The ANPs and CNPs were shown to be co-localized with a specific lysosomal marker, thus confirming their presence in lysosomes. We showed that tailoring the surface charge of the nanoparticles has a great impact on their internalization.

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Title: Effect of surface charge on the cellular uptake of fluorescent magnetic nanoparticles
Authors: Slavko Kralj
Matija Rojnik
Rok Romih
Marko Jagodič
Janko Kos
Darko Makovec
Publication date: 01-10-2012
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 10/2012
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-012-1151-7

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