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Journal of Crop Health

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05-04-2019 | Original Article

Effects of Arbuscular Mycorrhizal Fungi and Rhizobia Symbiosis on the Tolerance of Medicago Sativa to Salt Stress

Authors: R. Ben Laouane, A. Meddich, N. Bechtaoui, K. Oufdou, S. Wahbi

Published in: Journal of Crop Health | Issue 2/2019

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Abstract

Alfalfa is the most forage crop cultivated in Mediteranean area where salinity is one of the main limiting factors for its production. Thus, a consortium of arbuscular mycorrhizal fungi (AM) isolated from the Aoufous palmeraie, Morocco, and autochthonous rhizobia strain (RhLO1) or their combination (AM + RhLO1) were tested for their efficiency to improve alfalfa tolerance to salinity. The grass waste compost was given as a source of organic matter to the soil. Antioxidant status, physiological parameters and growth of alfalfa were evaluated. The results showed that the highest alfalfa growth was reached in plants dually inoculated particularly under non-stress conditions. Dual inoculation also enhanced the stomatal conductance in control plants, while the nodule number and mycorrhizal infection were enhanced under both conditions. Salt stress did not affect the efficiency of photosystem II. However, single or dual inoculation increased this efficiency. RhLO1 or AM + RhLO1 symbiosis could alleviate the deleterious effects of salinity on alfalfa. They could maintain higher leaf water potential (LWP Ψ), reducing the electrolyte leakages and malondialdehyde (MDA) content, inducing the accumulation of compatible osmolytes (sugar) and antioxidant enzymes as defense strategy. Thus, these autochthonous microorganisms were effective for alfalfa plants to alleviate salinity damage and improve plant growth and productivity.

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Title: Effects of Arbuscular Mycorrhizal Fungi and Rhizobia Symbiosis on the Tolerance of Medicago Sativa to Salt Stress
Authors: R. Ben Laouane
A. Meddich
N. Bechtaoui
K. Oufdou
S. Wahbi
Publication date: 05-04-2019
Publisher: Springer Berlin Heidelberg
Published in: Journal of Crop Health / Issue 2/2019
Print ISSN: 2948-264X
Electronic ISSN: 2948-2658
DOI: https://doi.org/10.1007/s10343-019-00461-x

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