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Journal of Materials Science

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04-03-2020 | Advanced Nano Materials

Electrospun PVA nanoscaffolds associated with propolis nanoparticles with wound healing activity

Authors: Thaís B. Alberti, Daniela S. Coelho, Manuel de Prá, Marcelo Maraschin, Beatriz Veleirinho

Published in: Journal of Materials Science | Issue 23/2020

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Abstract

Tissue engineering and nanotechnology have developed polymeric nanofibrous scaffolds for tissue regeneration. Propolis is a featured natural product due to its biological properties, including enhancement of the wound healing process. This study developed a polymeric nanofibrous wound dressing of polyvinyl alcohol (PVA) and propolis, and evaluated both in vitro and in vivo its potential in the wound healing process. The optimization of the experimental conditions was performed based on the evaluation of electrospinning process stability and morphology of the resulting nanofibers. The resulting scaffolds were evaluated through cell culture studies, using murine NIH/3T3 fibroblasts as study model, and then in vivo in a diabetic non-contractile wound healing model. PVA scaffolds associated with nanoparticles showed an adequate fiber morphology and did not present any cytotoxicity to fibroblasts in vitro. Furthermore, PVA scaffold with propolis nanoparticles showed the best wound closure rate (68%) after 7 days in comparison with treated (54%) and untreated controls (20%). Withal, preliminary results showed that PVA scaffold associated with propolis nanoparticles has potential to be applied in tissue regeneration. Furthermore, this work features as innovation the development of a nanoscaffold/nanoparticles system, which can be used not only for propolis, but for any natural extract, especially for those insoluble in water.

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Alberti T, Coelho DS, Voytena A, Pitz H, de Pra M, Mazzarino L, Kuhnen S, Ribeiro-do-Valle MR, Maraschin M, Veleirinho B (2017) Nanotechnology: a promising tool towards wound healing. Curr Pharm Des 23(24):3515–3528CrossRef

Coelho DS, Veleirinho B, Alberti T, Maestri A, Yunes R, Dias PF, Maraschin M (2018) Electrospinning technology: designing nanofibers toward wound healing application. In: Clichici S, Filip A, Nascimento GM (eds) Nanofibers-from preparation to applications. IntechOpen, London

Ariga K, Jia X, Song J, Hsieh CT, Hsu Sh (2019) Materials nanoarchitectonics as cell regulators. ChemNanoMat 5(6):692–702CrossRef

Campos ACL, Borges-Branco A, Groth AK (2007) Cicatrização de feridas. ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) 20:51–58CrossRef

Robson MC, Steed DL, Franz MG (2001) Wound healing: biologic features and approaches to maximize healing trajectories. Curr Probl Surg 38(2):A1–A140. https://doi.org/10.1067/msg.2001.111167 CrossRef

Kobayashi J, Okano T (2019) Design of temperature-responsive polymer-grafted surfaces for cell sheet preparation and manipulation. Bull Chem Soc Jpn 92(4):817–824CrossRef

Nuttelman CR, Mortisen DJ, Henry SM, Anseth KS (2001) Attachment of fibronectin to poly (vinyl alcohol) hydrogels promotes NIH3T3 cell adhesion, proliferation, and migration. J Biomed Mater Res 57(2):217–223CrossRef

Choi JS, Leong KW, Yoo HS (2008) In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (EGF). Biomaterials 29(5):587–596. https://doi.org/10.1016/j.biomaterials.2007.10.012 CrossRef

Sill TJ, von Recum HA (2008) Electrospinning: applications in drug delivery and tissue engineering. Biomaterials 29(13):1989–2006. https://doi.org/10.1016/j.biomaterials.2008.01.011 CrossRef

10.

Thakur RA, Florek CA, Kohn J, Michniak BB (2008) Electrospun nanofibrous polymeric scaffold with targeted drug release profiles for potential application as wound dressing. Int J Pharm 364(1):87–93. https://doi.org/10.1016/j.ijpharm.2008.07.033 CrossRef

11.

Cai Z-x, Mo X-m, Zhang K-h, Fan L-p, Yin A-l, He C-l, Wang H-s (2010) Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications. Int J Mol Sci 11(9):3529–3539. https://doi.org/10.3390/ijms11093529 CrossRef

12.

Veleirinho B, Coelho DS, Dias PF, Maraschin M, Ribeiro-do-Valle RM, Lopes-da-Silva JA (2012) Nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/chitosan scaffolds for skin regeneration. Int J Biol Macromol 51(4):343–350CrossRef

13.

Veleirinho B, Berti FV, Dias PF, Maraschin M, Ribeiro-do-Valle RM, Lopes-da-Silva JA (2013) Manipulation of chemical composition and architecture of non-biodegradable poly (ethylene terephthalate)/chitosan fibrous scaffolds and their effects on L929 cell behavior. Mater Sci Eng C 33(1):37–46CrossRef

14.

Veleirinho B, Coelho DS, Dias PF, Maraschin M, Pinto R, Cargnin-Ferreira E, Peixoto A, Souza JA, Ribeiro-do-Valle RM, Lopes-da-Silva JA (2014) Foreign body reaction associated with pet and pet/chitosan electrospun nanofibrous abdominal meshes. PLoS ONE 9(4):e95293CrossRef

15.

Villanova JC, Oréfice RL, Cunha AS (2010) Pharmaceutical applications of polymers. Polímeros 20(1):51–64CrossRef

16.

Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL (2007) Nanofiber technology: designing the next generation of tissue engineering scaffolds. Adv Drug Deliv Rev 59(14):1413–1433. https://doi.org/10.1016/j.addr.2007.04.022 CrossRef

17.

An J, Chua CK, Yu T, Li H, Tan LP (2013) Advanced nanobiomaterial strategies for the development of organized tissue engineering constructs. Nanomedicine 8(4):591–602CrossRef

18.

Villanova JC, Oréfice RL, Cunha AS (2010) Aplicações farmacêuticas de polímeros. Polímeros Ciência e tecnologia 20(1):51–64CrossRef

19.

Yunes RA, Pedrosa RC, Cechinel Filho V (2001) Fármacos e fitoterápicos: a necessidade do desenvolvimento da indústria de fitoterápicos e fitofármacos no Brasil. Quim Nova 24(1):147–152CrossRef

20.

Marcucci MC (1995) Propolis: chemical composition, biological properties and therapeutic activity. Apidologie 26(2):83–99CrossRef

21.

Park YK, Alencar SM, Scamparini ARP, Aguiar CL (2002) Própolis produzida no sul do Brasil, Argentina e Uruguai: evidências fitoquímicas de sua origem vegetal. Ciênc Rural 32(6):997–1003CrossRef

22.

Salatino A, Teixeira ÉW, Negri G (2005) Origin and chemical variation of brazilian propolis. Evid Based Complement Altern Med 2(1):33–38. https://doi.org/10.1093/ecam/neh060 CrossRef

23.

Oliveira SKd, Silva DAd, Tomazzoli MM, de Souza AC, Severino RB, Pereira A, Maraschin M, Pinto MR, Cella I, Veleirinho MB (2011) Qualitative analysis of propolis from Santa Catarina, Brazil, by 1D-and 2D-NMR

24.

Maraschin M, Zeggio AR, Souza ACV, Ferreira AG, da Silva DA, Vieira FA, Cella I, Delgadillo I, Tomazzoli MM, Veleirinho MB (2012) Metabolomics and chemometrics as tools for chemo (bio) diversity analysis-maize landraces and propolis. INTECH Open Access Publisher, LondonCrossRef

25.

Aga H, Shibuya T, Sugimoto T, Kurimoto M, Nakajima S (1994) Isolation and identification of antimicrobial compounds in Brazilian propolis. Biosci Biotechnol Biochem 58(5):945–946CrossRef

26.

Park E-H, Kim S-H, Park S-S (1996) Anti-inflammatory activity of propolis. Arch Pharmacal Res 19(5):337–341CrossRef

27.

Banskota AH, Tezuka Y, Kadota S (2001) Recent progress in pharmacological research of propolis. Phytother Res 15(7):561–571CrossRef

28.

De Castro S (2001) Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. ARBS Annu Rev Biomed Sci 3:49–83

29.

Castaldo S, Capasso F (2002) Propolis, an old remedy used in modern medicine. Fitoterapia 73(Supplement 1):S1–S6. https://doi.org/10.1016/S0367-326X(02)00185-5 CrossRef

30.

McLennan SV, Bonner J, Milne S, Lo L, Charlton A, Kurup S, Jia J, Yue DK, Twigg SM (2008) The anti-inflammatory agent Propolis improves wound healing in a rodent model of experimental diabetes. Wound Repair Regen 16(5):706–713CrossRef

31.

Barbosa MH, Zuffi FB, Maruxo HB, Jorge LLR (2009) Ação terapêutica da própolis em lesões cutâneas. Acta Paulista de Enfermagem 22(3):318–322CrossRef

32.

Sehn E, Hernandes L, Franco SL, Gonçalves CCM, Baesso ML (2009) Dynamics of reepithelialisation and penetration rate of a bee propolis formulation during cutaneous wounds healing. Anal Chim Acta 635(1):115–120. https://doi.org/10.1016/j.aca.2009.01.019 CrossRef

33.

Abu-Seida AM (2015) Effect of propolis on experimental cutaneous wound healing in dogs. Vet Med Int. https://doi.org/10.1155/2015/672643 CrossRef

34.

Jaiswal M, Dudhe R, Sharma PK (2015) Nanoemulsion: an advanced mode of drug delivery system. 3 Biotech 5(2):123–127. https://doi.org/10.1007/s13205-014-0214-0 CrossRef

35.

Yy Duan, Jia J, Wang Sh, Yan W, Jin L, Zy Wang (2007) Preparation of antimicrobial poly (ϵ-caprolactone) electrospun nanofibers containing silver-loaded zirconium phosphate nanoparticles. J Appl Polym Sci 106(2):1208–1214CrossRef

36.

Losi P, Briganti E, Errico C, Lisella A, Sanguinetti E, Chiellini F, Soldani G (2013) Fibrin-based scaffold incorporating VEGF-and bFGF-loaded nanoparticles stimulates wound healing in diabetic mice. Acta Biomater 9(8):7814–7821CrossRef

37.

Xie Z, Paras CB, Weng H, Punnakitikashem P, Su L-C, Vu K, Tang L, Yang J, Nguyen KT (2013) Dual growth factor releasing multi-functional nanofibers for wound healing. Acta Biomater 9(12):9351–9359CrossRef

38.

Kumar PS, Abhilash S, Manzoor K, Nair S, Tamura H, Jayakumar R (2010) Preparation and characterization of novel β-chitin/nanosilver composite scaffolds for wound dressing applications. Carbohydr Polym 80(3):761–767CrossRef

39.

Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L (2013) Silver nanoparticle/chitosan oligosaccharide/poly (vinyl alcohol) nanofibers as wound dressings: a preclinical study. Int J Nanomed 8:4131–4145

40.

Augustine R, Dominic EA, Reju I, Kaimal B, Kalarikkal N, Thomas S (2014) Electrospun polycaprolactone membranes incorporated with ZnO nanoparticles as skin substitutes with enhanced fibroblast proliferation and wound healing. RSC Adv 4(47):24777–24785. https://doi.org/10.1039/C4RA02450H CrossRef

41.

Sikareepaisan P, Suksamrarn A, Supaphol P (2007) Electrospun gelatin fiber mats containing a herbal—Centella asiatica—extract and release characteristic of asiaticoside. Nanotechnology 19(1):015102–015112CrossRef

42.

Suganya S, Senthil Ram T, Lakshmi B, Giridev V (2011) Herbal drug incorporated antibacterial nanofibrous mat fabricated by electrospinning: an excellent matrix for wound dressings. J Appl Polym Sci 121(5):2893–2899CrossRef

43.

Karri VVSR, Kuppusamy G, Talluri SV, Mannemala SS, Kollipara R, Wadhwani AD, Mulukutla S, Raju KRS, Malayandi R (2016) Curcumin loaded chitosan nanoparticles impregnated into collagen-alginate scaffolds for diabetic wound healing. Int J Biol Macromol 93:1519–1529CrossRef

44.

Ranjbar-Mohammadi M, Rabbani S, Bahrami SH, Joghataei M, Moayer F (2016) Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly (ε-caprolactone) electrospun nanofibers. Mater Sci Eng C 69:1183–1191CrossRef

45.

Alberti T, Coelho D, Voytena A, Iacovski R, Mazzarino L, Maraschin M, Veleirinho B (2019) Effect of propolis nanoparticles on early-stage wound healing in a diabetic noncontractile wound model. Nanotechnol Adv Mater Sci 2(1):10

46.

International Organization for Standardization (2008) ISO 10995: 2008: quality management systems-requirements. International Organization for Standardization, Geneva

47.

OECD E (2010) Guidance document on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests. OECD Ser Test Assess 20:1–54

48.

Vareda PMP (2013) Avaliação da atividade hipoglicemiante do extrato de Myrcia bella em camundongos diabéticos por estreptozotocina

49.

Wang X, Ge J, Tredget EE, Wu Y (2013) The mouse excisional wound splinting model, including applications for stem cell transplantation. Nat Protoc 8(2):302–309CrossRef

50.

Peppas NA, Keys KB, Torres-Lugo M, Lowman AM (1999) Poly (ethylene glycol)-containing hydrogels in drug delivery. J Control Release 62(1–2):81–87CrossRef

51.

Schmedlen RH, Masters KS, West JL (2002) Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering. Biomaterials 23(22):4325–4332CrossRef

52.

Sailaja GS, Sreenivasan K, Yokogawa Y, Kumary TV, Varma HK (2009) Bioinspired mineralization and cell adhesion on surface functionalized poly(vinyl alcohol) films. Acta Biomater 5(5):1647–1655. https://doi.org/10.1016/j.actbio.2008.12.005 CrossRef

53.

Reneker DH, Chun I (1996) Nanometre diameter fibres of polymer, produced by electrospinning. Nanotechnology 7(3):216–223CrossRef

54.

Veleirinho MBdR (2007) Desenvolvimento de membranas nanofibrosas por electrospinning: potencial aplicação na clarificação de sumos

55.

Reneker DH, Yarin AL (2008) Electrospinning jets and polymer nanofibers. Polymer 49(10):2387–2425. https://doi.org/10.1016/j.polymer.2008.02.002 CrossRef

56.

Park YK, Ikegaki M, Abreu JdS, Alcici NMF (1998) Estudo da preparação dos extratos de própolis e suas aplicações. Ciênc Tecnol Aliment 18(3):313–318CrossRef

57.

Bankova V (2005) Chemical diversity of propolis and the problem of standardization. J Ethnopharmacol 100(1):114–117CrossRef

58.

Fasolo D, Schwingel L, Holzschuh M, Bassani V, Teixeira H (2007) Validation of an isocratic LC method for determination of quercetin and methylquercetin in topical nanoemulsions. J Pharm Biomed Anal 44(5):1174–1177CrossRef

59.

Veleirinho B, Ribeiro-do-Valle R, Lopes-da-Silva JA (2011) Processing conditions and characterization of novel electrospun poly (3-hydroxybutyrate-co-hydroxyvalerate)/chitosan blend fibers. Mater Lett 65(14):2216–2219CrossRef

60.

Veleirinho B, Lopes-da-Silva J (2009) Application of electrospun poly (ethylene terephthalate) nanofiber mat to apple juice clarification. Process Biochem 44(3):353–356CrossRef

61.

Mansur HS, Oréfice RL, Mansur AA (2004) Characterization of poly (vinyl alcohol)/poly (ethylene glycol) hydrogels and PVA-derived hybrids by small-angle X-ray scattering and FTIR spectroscopy. Polymer 45(21):7193–7202CrossRef

62.

Alipour SM, Nouri M, Mokhtari J, Bahrami SH (2009) Electrospinning of poly (vinyl alcohol)–water-soluble quaternized chitosan derivative blend. Carbohydr Res 344(18):2496–2501CrossRef

63.

Aramwit P, Siritientong T, Kanokpanont S, Srichana T (2010) Formulation and characterization of silk sericin–PVA scaffold crosslinked with genipin. Int J Biol Macromol 47(5):668–675CrossRef

64.

Pal K, Banthia A, Majumdar D (2007) Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications. AAPS PharmSciTech 8(1):E142–E146. https://doi.org/10.1208/pt080121 CrossRef

65.

Hehenberger K, Hansson A (1997) High glucose-induced growth factor resistance in human fibroblasts can be reversed by antioxidants and protein kinase C-inhibitors. Cell Biochem Funct 15(3):197–201CrossRef

66.

Naguib G, Al-Mashat H, Desta T, Graves DT (2004) Diabetes prolongs the inflammatory response to a bacterial stimulus through cytokine dysregulation. J Invest Dermatol 123(1):87–92CrossRef

67.

Lustosa SR, Galindo AB, Nunes LC, Randau KP, Rolim Neto PJ (2008) Própolis: atualizações sobre a química e a farmacologia. Rev Bras Farmacogn 18(3):447–454CrossRef

68.

Moura SALd, Negri G, Salatino A, Lima LDdC, Dourado LPA, Mendes JB, Andrade SP, Ferreira MAND, Cara DC (2011) Aqueous extract of Brazilian green propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges. Evid Based Complement Altern Med. https://doi.org/10.1093/ecam/nep112 CrossRef

69.

Kim JI, Pant HR, Sim H-J, Lee KM, Kim CS (2014) Electrospun propolis/polyurethane composite nanofibers for biomedical applications. Mater Sci Eng C 44:52–57. https://doi.org/10.1016/j.msec.2014.07.062 CrossRef

70.

Voss GT, Gularte MS, Vogt AG, Giongo JL, Vaucher RA, Echenique JVZ, Soares MP, Luchese C, Wilhelm EA, Fajardo AR (2018) Polysaccharide-based film loaded with vitamin C and propolis: a promising device to accelerate diabetic wound healing. Int J Pharm 552(1):340–351. https://doi.org/10.1016/j.ijpharm.2018.10.009 CrossRef

71.

Marquele-Oliveira F, da Silva Barud H, Torres EC, Machado RTA, Caetano GF, Leite MN, Frade MAC, Ribeiro SJL, Berretta AA (2019) Development, characterization and pre-clinical trials of an innovative wound healing dressing based on propolis (EPP-AF^®)-containing self-microemulsifying formulation incorporated in biocellulose membranes. Int J Biol Macromol 136:570–578. https://doi.org/10.1016/j.ijbiomac.2019.05.135 CrossRef

72.

Eskandarinia A, Kefayat A, Rafienia M, Agheb M, Navid S, Ebrahimpour K (2019) Cornstarch-based wound dressing incorporated with hyaluronic acid and propolis: in vitro and in vivo studies. Carbohydr Polym 216:25–35. https://doi.org/10.1016/j.carbpol.2019.03.091 CrossRef

73.

Krupp T, dos Santos BD, Gama LA, Silva JR, Arrais-Silva WW, de Souza NC, Américo MF, de Souza Souto PC (2019) Natural rubber—propolis membrane improves wound healing in second-degree burning model. Int J Biol Macromol 131:980–988. https://doi.org/10.1016/j.ijbiomac.2019.03.147 CrossRef

74.

Khoshnevisan K, Maleki H, Samadian H, Doostan M, Khorramizadeh MR (2019) Antibacterial and antioxidant assessment of cellulose acetate/polycaprolactone nanofibrous mats impregnated with propolis. Int J Biol Macromol 140:1260–1268. https://doi.org/10.1016/j.ijbiomac.2019.08.207 CrossRef

75.

Chao CY, Mani MP, Jaganathan SK (2018) Engineering electrospun multicomponent polyurethane scaffolding platform comprising grapeseed oil and honey/propolis for bone tissue regeneration. PLoS ONE 13(10):e0205699. https://doi.org/10.1371/journal.pone.0205699 CrossRef

76.

Jaganathan SK, Mani MP, Rathanasamy R, Prabhakaran P (2018) Fabrication and characterization of tailor-made novel electrospun fibrous polyurethane scaffolds decorated with propolis and neem oil for tissue engineering applications. J Ind Text. https://doi.org/10.1177/1528083718808787 CrossRef

77.

Moghaddam AB, Shirvani B, Aroon MA, Nazari T (2018) Physico-chemical properties of hybrid electrospun nanofibers containing polyvinylpyrrolidone (PVP), propolis and aloe vera. Mater Res Express 5(12):125404. https://doi.org/10.1088/2053-1591/aae0bf CrossRef

Title: Electrospun PVA nanoscaffolds associated with propolis nanoparticles with wound healing activity
Authors: Thaís B. Alberti
Daniela S. Coelho
Manuel de Prá
Marcelo Maraschin
Beatriz Veleirinho
Publication date: 04-03-2020
Publisher: Springer US
Published in: Journal of Materials Science / Issue 23/2020
Print ISSN: 0022-2461
Electronic ISSN: 1573-4803
DOI: https://doi.org/10.1007/s10853-020-04502-z

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