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Polymer Bulletin
Published in: Polymer Bulletin 3/2023

02-04-2022 | Original Paper

Evaluation of the effect of magnetic nanoparticles on extraction of genomic DNA of Escherichia coli

Authors: Farzaneh Firoozeh, Abed Neshan, Azad Khaledi, Mohammad Zibaei, Ala Amiri, Ali Sobhani, Farzad Badmasti, Vajihe Sadat Nikbin

Published in: Polymer Bulletin | Issue 3/2023

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Abstract

The initial step in molecular biology and genetic engineering fields are the finding of optimal methods for the extraction of the genomic DNA from microorganisms and cells. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is a novel method for the isolation of nucleic acid. In the present study, the synthesized magnetic nanoparticles were used to extract the genomic DNA of Escherichia coli. In this study, the naked (Fe3O4) and coated (Fe3O4/SiO2; Fe3O4/SiO2/TiO2) magnetic nanoparticles were synthesized by precipitation methods. The purity of the product was assessed using energy-dispersive x-ray spectroscopy (EDS) and x-ray crystallography. The morphology and size of specimens were observed via field emission scanning electron microscopy (FESEM). The efficacy of the synthesized nanoparticles in genomic DNA extraction from Escherichia coli strain ATCC25922 was compared. The quality of the resulting DNA was analyzed by NanoDrop spectrophotometer, agarose gel electrophoresis, and PCR assays. According to the findings of SEM, the size of Fe3O4/SiO2/TiO2 magnetic nanoparticles was about 43.25 nm (Fe3O4/SiO2/TiO2). XRD analysis showed that the product nanoparticles were synthesized without impurities. The extraction results also showed that the purity and concentration of extracted genomic DNA by coated magnetic nanoparticles in comparison with naked magnetic nanoparticles were higher. In addition, the use of 0.4 mg of nanoparticles and 80% polyethylene glycol were optimal conditions for achieving a higher quality of DNA. According to the obtained results, the coated magnetic nanoparticles Fe3O4/SiO2/TiO2 can be used to extract genomic DNA of high quality from gram-negative bacteria.
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Literature
1.
Vesty A, Biswas K, Taylor MW, Gear K, Douglas RG (2017) Evaluating the impact of DNA extraction method on the representation of human oral bacterial and fungal communities. PloS one 12(1):e0169877CrossRefPubMedPubMedCentral
2.
Mocan T, Matea CT, Pop T, Mosteanu O, Buzoianu AD, Puia C et al (2017) Development of nanoparticle-based optical sensors for pathogenic bacterial detection. J nanobiotechnol 15(1):1–14CrossRef
3.
Akbari M, Aetemady A, Firoozeh F, Yaseliani M (2017) Synthesis of AgO–TiO2 nanocomposite through a simple method and its antibacterial activities. J Mater Sci: Mater Electron 28(14):10245–10249
4.
Neamati F, Firoozeh F, Saffari M, Zibaei M (2015) Virulence genes and antimicrobial resistance pattern in uropathogenic Escherichia coli isolated from hospitalized patients in Kashan, Iran. Jundishapur J Microbiol 8(2):23–28
5.
6.
Tan SC, Yiap BC (2009) DNA, RNA, and protein extraction: the past and the present. J Biomed Biotechnol 12(4):161–172
7.
8.
Gong R, Li S (2014) Extraction of human genomic DNA from whole blood using a magnetic microsphere method. Int J Nanomed 9:3781CrossRef
9.
Rohland N, Hofreiter M (2007) Comparison and optimization of ancient DNA extraction. Biotechniques 42(3):343–352CrossRefPubMed
10.
Danthanarayana AN, Manatunga DC, De Silva RM, Chandrasekharan NV, Nalin De Silva K (2018) Magnetofection and isolation of DNA using polyethyleneimine functionalized magnetic iron oxide nanoparticles. Royal Soc open sci 5(12):181369CrossRef
11.
Wierucka M, Biziuk M (2014) Application of magnetic nanoparticles for magnetic solid-phase extraction in preparing biological, environmental and food samples. TrAC, Trends Anal Chem 59:50–58CrossRef
12.
Saiyed Z, Ramchand C, Telang S (2008) Isolation of genomic DNA using magnetic nanoparticles as a solid-phase support. J Phys: Condens Matter 20(20):204153PubMed
13.
Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK (2018) Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol 9(1):1050–1074CrossRefPubMedPubMedCentral
14.
15.
Rahnama H, Sattarzadeh A, Kazemi F, Ahmadi N, Sanjarian F, Zand Z (2016) Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction. Anal Biochem 513:68–76CrossRefPubMed
16.
Wei Y, Han B, Hu X, Lin Y, Wang X, Deng X (2012) Synthesis of Fe3O4 nanoparticles and their magnetic properties. Proced Eng 27:632–637CrossRef
17.
Abbas M, Rao BP, Islam MN, Naga S, Takahashi M, Kim C (2014) Highly stable-silica encapsulating magnetite nanoparticles (Fe3O4/SiO2) synthesized using single surfactantless-polyol process. Ceram Int 40(1):1379–1385CrossRef
18.
Farnleitner AH, Kreuzinger N, Kavka GG, Grillenberger S, Rath J, Mach RL (2000) Simultaneous detection and differentiation of Escherichia coli populations from environmental freshwaters by means of sequence variations in a fragment of the β-D-glucuronidase gene. Appl Environ Microbiol 66(4):1340–1346CrossRefPubMedPubMedCentral
19.
Cortes-Chosan D, Griffits R (2014) Methods for extracting genomic DNA from whole blood samples: current perspectives. J. Biorepository Sci App Med 2:1–9
20.
Min JH, Woo M-K, Yoon HY, Jang JW, Wu JH, Lim C-S et al (2014) Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid. Anal Biochem 447:114–118CrossRefPubMed
21.
Yoza B, Matsumoto M, Matsunaga T (2002) DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound. J Biotechnol 94(3):217–224CrossRefPubMed
22.
Frerix A, Schönewald M, Geilenkirchen P, Müller M, Kula M-R, Hubbuch J (2006) Exploitation of the coil− globule plasmid DNA transition induced by small changes in temperature, pH salt, and poly (ethylene glycol) compositions for directed partitioning in aqueous two-phase systems. Langmuir 22(9):4282–4290CrossRefPubMed
23.
Metadata
Title
Evaluation of the effect of magnetic nanoparticles on extraction of genomic DNA of Escherichia coli
Authors
Farzaneh Firoozeh
Abed Neshan
Azad Khaledi
Mohammad Zibaei
Ala Amiri
Ali Sobhani
Farzad Badmasti
Vajihe Sadat Nikbin
Publication date
02-04-2022
Publisher
Springer Berlin Heidelberg
Published in
Polymer Bulletin / Issue 3/2023
Print ISSN: 0170-0839
Electronic ISSN: 1436-2449
DOI
https://doi.org/10.1007/s00289-022-04196-0

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