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World of Proteins: Structure-Function Relationships and Engineering Techniques Read chapter Read first chapter

2017 | OriginalPaper | Chapter

2. Expanding the Synthetic Protein Universe by Guided Evolutionary Concepts

Authors : Krishna Mohan Poluri, Khushboo Gulati

Published in: Protein Engineering Techniques

Publisher: Springer Singapore

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Abstract

The genetic information content of a cell is maintained by the sequence composition of the DNA. The changes in the nucleotide content will potentially alter its transcriptional and translational events thus influencing the characteristics of the newly synthesized proteins. These nature’s alterations can be helpful in the evolution of proteins with novel/improved functionalities or they can contribute to the pathogenesis with loss of native functionalities. Unraveling the logistics of such a molecular evolutionary process is resourceful to strategically implement it for the benefit of the mankind through laboratory techniques. The laboratory process of synthesizing novel proteins in a constructive way through evolutionary guided principles is called “directed evolution”. This chapter will discuss various techniques, their strengths and pitfalls that are developed under the umbrella of directed evolution scheme.

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previous chapter World of Proteins: Structure-Function Relationships and Engineering Techniques
next chapter Rational Designing of Novel Proteins Through Computational Approaches
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Metadata
Title
Expanding the Synthetic Protein Universe by Guided Evolutionary Concepts
Authors
Krishna Mohan Poluri
Khushboo Gulati
Copyright Year
2017
Publisher
Springer Singapore
Book
Protein Engineering Techniques

Print ISBN: 978-981-10-2731-4

Electronic ISBN: 978-981-10-2732-1

Copyright Year: 2017

DOI
https://doi.org/10.1007/978-981-10-2732-1_2

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