Top

Journal of Nanoparticle Research

Published in:

01-07-2011 | Research paper

Inhibition effects of protein-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth

Authors: Ying Cao, Hua-Jie Wang, Cui Cao, Yuan-Yuan Sun, Lin Yang, Bao-Qing Wang, Jian-Guo Zhou

Published in: Journal of Nanoparticle Research | Issue 7/2011

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

In this article, a facile and environmentally friendly method was applied to fabricate BSA-conjugated amorphous zinc sulfide (ZnS) nanoparticles using bovine serum albumin (BSA) as the matrix. Transmission electron microscopy analysis indicated that the stable and well-dispersed nanoparticles with the diameter of 15.9 ± 2.1 nm were successfully prepared. The energy dispersive X-ray, X-ray powder diffraction, Fourier transform infrared spectrograph, high resolution transmission electron microscope, and selected area electron diffraction measurements showed that the obtained nanoparticles had the amorphous structure and the coordination occurred between zinc sulfide surfaces and BSA in the nanoparticles. In addition, the inhibition effects of BSA-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth were described in detail by cell viability analysis, optical and electron microscopy methods. The results showed that BSA-conjugated amorphous zinc sulfide nanoparticles could inhibit the metabolism and proliferation of human hepatocellular carcinoma cells, and the inhibition was dose dependent. The half maximal inhibitory concentration (IC50) was 0.36 mg/mL. Overall, this study suggested that BSA-conjugated amorphous zinc sulfide nanoparticles had the application potential as cytostatic agents and BSA in the nanoparticles could provide the modifiable site for the nanoparticles to improve their bioactivity or to endow them with the target function.

previous article Gold nanorod stabilized by thiolated chitosan as photothermal absorber for cancer cell treatment

next article Sonochemical synthesis and application of rhodium–graphene nanocomposite

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Anand KV, Chinnu MK, Kumar RM, Mohan R, Jayavel R (2010) Thermal stability and optical properties of HMTA capped zinc sulfide nanoparticles. J Alloys Compd 496:665–668CrossRef

Battaglia A, Ghidini S, Campanini G, Spaggiari R (2005) Heavy metal contamination in little owl (Athene noctua) and common buzzard (Buteo buteo) from northern Italy. Ecotoxicol Environ Saf 60:61–66CrossRef

Brannon-Peppas L, Blanchette JO (2004) Nanoparticle and targeted systems for cancer therapy. Adv Drug Deliv Rev 56:1649–1659CrossRef

Cheng Y, Samia AC, Malcolm JL, Kenney E, Resnick A, Burda C (2010) Delivery and efficacy of a cancer drug as a function of the bond to the gold nanoparticle surface. Langmuir 26(4):2248–2255CrossRef

Goswami N, Sen P (2004) Water-induced stabilization of ZnS nanoparticles. Solid State Commun 132:791–794CrossRef

Guo YM, Zhang J, Yang L, Wang HJ, Wang FF, Zheng Z (2010) Syntheses of amorphous and crystalline cupric sulfide nanoparticles and study on the specific activities on different cells. Chem Commun 46:3493–3495CrossRef

Jamieson T, Bakhshi R, Petrova D, Pocock R, Imani M, Seifalian AM (2007) Biological applications of quantum dots. Biomaterials 28:4717–4732CrossRef

Jiang CL, Zhang WQ, Zou GF, Yu WC, Qian YT (2007) Hydrothermal synthesis and characterization of ZnS microspheres and hollow nanospheres. Mater Chem Phys 103:24–27CrossRef

Khiew PS, Radiman S, Huang NM, Ahmad MS, Nadarajah K (2005) Preparation and characterization of ZnS nanoparticles synthesized from chitosan laurate micellar solution. Mater Lett 59:989–993CrossRef

Kusakabe T, Nakajima K, Nakazato K, Suzuki K, Takada H, Satoh T, Oikawa M, Arakawa K, Nagamine T (2008) Changes of heavy metal, metallothionein and heat shock proteins in Sertoli cells induced by cadmium exposure. Toxicol In Vitro 22:1469–1475CrossRef

Lee SS, Byun KT, Park JP, Kim SK, Lee JC, Chang SK, Kwak HY, Shim IW (2008) Homogeneous ZnS coating onto TiO₂ nanoparticles by a simple one pot sonochemical method. Chem Eng J 139:194–197CrossRef

Mathew ME, Mohan JC, Manzoor K, Nair SV, Tamura H, Jayakumar R (2010) Folate conjugated carboxymethyl chitosan-manganese doped zinc sulphide nanoparticles for targeted drug delivery and imaging of cancer cells. Carbohydr Polym 80:442–448CrossRef

Mehdizadeh M, Kermanian F, Farjah G (2008) Schwann cell injuries of radial nerve after lead (Pb) exposure in rats. Pathophysiology 15:13–17CrossRef

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63CrossRef

Nam J, Won N, Jin H, Chung H, Kim S (2009) pH-induced aggregation of gold nanoparticles for photothermal cancer therapy. J Am Chem Soc 131(38):13639–13645CrossRef

Ni Shuilleabhain SN, Mothersill C, Sheehan D, O’Brien NM, O’Halloran J, Van Pelt FNAM, Davoren M (2004) In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines. Toxicol In Vitro 18:365–376CrossRef

Perez-Lopez M, Cid F, Oropesa AL, Hidalgo LE, Lopez-Beceiro A, Soler F (2006) Heavy metal and arsenic content in seabirds affected by the Prestige oil spill on the Galician coast (NW Spain). Sci Total Environ 359(1–3):209–220

Rema Devi BS, Raveendran R, Vaidyan AV (2007) Synthesis and characterization of Mn²⁺-doped ZnS nanoparticles. Pramana. J Phys 68(4):679–687

Sanpera C, Morera M, Ruiz X, Jover L (2000) Variability of mercury and selenium levels in clutches of Audouin’s gulls (Larus audouinii) breeding at the Chafarinas Islands, Southwest Mediterranean. Arch Environ Contam Toxicol 39:119–123

Sayes CM, Wahi R, Kurian PA, Liu Y, West JL, Ausman KD, Warheit DB, Colvin VL (2006) Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol Sci 92:174–185CrossRef

Schroeder JE, Shweky I, Shmeeda H, Banin U, Gabizon A (2007) Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles. J Control Release 124(1–2):28–34CrossRef

Seoudi R, Shabaka A, Eisa WH, Anies B, Farage NM (2010) Effect of the prepared temperature on the size of CdS and ZnS nanoparticles. Physica B 405:919–924CrossRef

Skrabalak SE, Chen J, Au L, Lu X, Li X, Xia Y (2007) Gold nanocages for biomedical applications. Adv Mater 19:3177–3184CrossRef

Steinebach OM, Wolterbeek HT (1993) Effects of zinc on rat hepatoma HTC cells and primary cultured rat hepatocytes. Toxicol Appl Pharmacol 118:245–254CrossRef

Valee BL, Auld DA (1990) Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry 29(24):5647–5659CrossRef

Walther UI (2004) Changes in the glutathione system of lung cell lines after treatment with hydrocortisone. Arch Toxicol 78:402–409CrossRef

Walther UI, Walther SC, Temruck O (2007) Effect of enlarged glutathione on zinc-mediated toxicity in lung-derived cell lines. Toxicol In Vitro 21:380–386CrossRef

Wang B, Feng WY, Wang TC, Jia G, Wang M, Shi JW, Zhang F, Zhao YL, Chai ZF (2006) Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice. Toxicol Lett 161:115–123CrossRef

Wang HJ, Yang L, Yang HY, Wang K, Yao WG, Jiang K, Huang XL, Zheng Z (2010) Antineoplastic activities of protein-conjugated silver sulfide nano-crystals with different shapes. J Inorg Biochem 104:87–91CrossRef

Warheit DB, Webb TR, Sayes CM, Colvin VL, Reed KL (2006) Pulmonary instillation studies with nanoscale TiO₂ rods and dots in rats: toxicity is not dependent upon particle size and surface area. Toxicol Sci 91:227–236CrossRef

Warheit DB, Webb TR, Colvin VL, Reed KL, Sayes CM (2007) Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics. Toxicol Sci 95:270–280CrossRef

Wihelm B, Walther UI, Fichtl B (2001) Effects of zinc chloride on glutathione and glutathione synthesis rates in various lung cell lines. Arch Toxicol 75:388–394CrossRef

Yang L, Shen Q, Zhou J, Jiang K (2006) Biomimetic synthesis of CdS nanocrystals in aqueous solution of pepsin. Mater Chem Phys 98:125–130CrossRef

Yang L, Wang HJ, Yang HY, Liu SH, Zhang BF, Wang K, Ma XM, Zheng Z (2008) Shape-controlled synthesis of protein-conjugated silver sulfide nanocrystals and study on the inhibition of tumor cell viability. Chem Commun 2995–2997

Zhang J, Ma X, Guo Y, Yang L, Shen Q, Wang H, Ma Z (2010) Size-controllable preparation of bovine serum albumin-conjugated. Mater Chem Phys 119:112–117CrossRef

Title: Inhibition effects of protein-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth
Authors: Ying Cao
Hua-Jie Wang
Cui Cao
Yuan-Yuan Sun
Lin Yang
Bao-Qing Wang
Jian-Guo Zhou
Publication date: 01-07-2011
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 7/2011
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-010-0163-4

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 7/2011

Future conferences and symposia (July 2011–)

Biodistribution of co-exposure to multi-walled carbon nanotubes and graphene oxide nanoplatelets radiotracers

Mn–ferrite nanoparticles via reverse microemulsions: synthesis and characterization

Simple and environmentally friendly preparation and size control of silver nanoparticles using an inhomogeneous system with silver-containing glass powder

Colloidal stability of silver nanoparticles in biologically relevant conditions

Biotoxicity of nanoparticles: effect of natural organic matter

Premium Partners