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Journal of Sol-Gel Science and Technology
Published in: Journal of Sol-Gel Science and Technology 3/2014

01-09-2014 | Original Paper

Preparation and characterization of protein molecularly imprinted polysiloxane using mesoporous calcium silicate as matrix by sol–gel technology

Authors: Lingzhi Feng, Bohong Kan, Kongyin Zhao, Junfu Wei, Dunwan Zhu, Linhua Zhang

Published in: Journal of Sol-Gel Science and Technology | Issue 3/2014

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Abstract

Calcium silicate particles containing mesoporous SiO2 on the surface (CaSiO3@SiO2) were prepared by acid modification of calcium silicate with diluted hydrochloric acid. Bovine serum albumin (BSA) molecularly imprinted polysiloxane (MIP) was synthesized using silane as the functional monomer, BSA as the template and CaSiO3@SiO2 particles as the matrix in an aqueous phase. SEM, granulometry, FT-IR and BET analysis were used to characterize the protein MIP. Influence factors on the rebinding capacity of the MIP were investigated, such as the pH in treating CaSiO3, eluent type and silane proportion. The mass of BSA loading and rebinding on CaSiO3@SiO2 and MIP was investigated. The adsorption and recognition properties of the MIP were evaluated and the results showed that the MIP exhibited an obvious improvement in terms of rebinding capacity for BSA as compared with non-imprinted polysiloxane (NIP). BSA imprinted polysiloxane could recognize the template protein by using Lys, Ova, Hb, and Glo as control proteins, and the selectivity factor (β) was above 2.3. The rebinding capacity of BSA imprinted polysiloxane for BSA reached 81.31 mg/g, which was 2.25 times of NIP.
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Metadata
Title
Preparation and characterization of protein molecularly imprinted polysiloxane using mesoporous calcium silicate as matrix by sol–gel technology
Authors
Lingzhi Feng
Bohong Kan
Kongyin Zhao
Junfu Wei
Dunwan Zhu
Linhua Zhang
Publication date
01-09-2014
Publisher
Springer US
Published in
Journal of Sol-Gel Science and Technology / Issue 3/2014
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI
https://doi.org/10.1007/s10971-014-3396-3

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