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Journal of Nanoparticle Research

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01-07-2013 | Research Paper

Probing the fungicidal property of CdS quantum dots on Saccharomyces cerevisiae and Candida utilis using MALDI-MS

Authors: Muthu Manikandan, Hui-Fen Wu

Published in: Journal of Nanoparticle Research | Issue 7/2013

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Abstract

For the first time, we report the successful application of inhouse synthesized CdS quantum dots (QDs) with particle sizes between 1 and 7 nm exhibiting excellent fungicidal activity based on the interactions with Saccharomyces cerevisiae and Candida utilis. The growth curves and the growth rates of both fungi were established in the presence of three varying concentrations of CdS QDs. It was observed that the CdS QDs were highly inhibitory even at the lowest concentration of 10 mg/L used in this study, while the untreated control cells followed a normal growth pattern in the cases of both Saccharomyces and Candida. MALDI-MS was applied to substantiate the observations obtained by direct cell count method. It was observed that the trend observed in the case of Saccharomyces and Candida was well-represented in the MALDI-MS spectra. This study proposes a mechanism for the first time based on MALDI-MS results, that the CdS QDs interact with the extracellular polymeric substances (EPS) and remove small molecules from EPS layer; on the other hand, it was observed that CdS QDs at all concentrations lead to enrichment of protein signals in MALDI-MS. We have substantiated these results by quantifying the EPS in the control and treated cells and also using TEM to further confirm the results.

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Aboulaich A et al (2012) Physicochemical properties and cellular toxicity of (poly) aminoalkoxysilanes-functionalized ZnO quantum dots. Nanotechnology 23:335101 1–9CrossRef

Amantonico A, Urban PL, Fagerer SR, Balabin RM, Zenobi R (2010) Anal Chem 82:7394–7400CrossRef

Biju V et al (2007) Quantum dot-Insect neuropeptide conjugates for fluorescence imaging, transfection, and nucleus targeting of living cells. Langmuir 23:10254–10267CrossRef

Bougnoux ME, Gueho E, Potocka PC (1999) Resolutive Candida utilis fungemia in a nonneutropenic patient. J Clin Microbio 31:1644–1645

Cai R et al (1992) Induction of cytotoxicity by photoexcited TiO₂ particles. Cancer Res 52:2346–2348

Chalmers NI et al (2007) Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms. Appl Environ Microbiol 73:630–636CrossRef

Chan WCW, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018CrossRef

Cho SJ et al (2007) Long-term exposure to CdTe quantum dots causes functional impairments in live cells. Langmuir 23:1974–1980CrossRef

Colvin VL (2003) The potential environmental impact of engineered nanomaterials. Nat Biotechnol 21:1166–1170CrossRef

Constantine CA et al (2003) Layer-by-layer biosensor assembly incorporating functionalized quantum dots. Langmuir 19:9863–9867CrossRef

Costerton JW, Stewart PS, Greenberg EB (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322CrossRef

Donaldson K, Stone V, Clouter A, Renwick L, MacNee W (2001) Ultra fine particles. Occup Environ Med 58:211–216CrossRef

Du Z, Yang R, Guo Z, Song Y, Wang J (2002) Universal sample preparation method for characterization of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Anal Chem 74:5487–5491CrossRef

Dwarakanatha S et al (2007) Mechanism of antimicrobial activity of CdTe quantum dots. Folia Microbiol. (Prague) 52:31–34CrossRef

Edgar R et al (2006) High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes. Proc Natl Acad Sci USA 103:4841–4845CrossRef

Fenselau C, Demirev P (2001) Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrom 20:157–171CrossRef

Ferreira L, Juanes FS, Munoz-Bellido JL, Gonza′lez-Buitrago M (2010) Rapid identification of bacteria from positive blood culture bottles. J Clin Microbiol Infect. doi:10.1111/j.1469-0691.2010.03339.x

Flemming HC, Wingender J, Griegbe J, Mayer C (2000) Physicochemical properties of biofilms, p. 2000. In: Evans LV (ed) Biofilms: recent advances in their study and control. Harwood Academic Publishers, Amsterdam, pp 19–34

Glynne Ivo (2004) Gut DNA analysis by MALDI-TOF mass spectrometry. Hum Mutat 23(5):437–441CrossRef

Gopal J, Wu HF, Raju G (2011) Quantifying the degradation of extracellular polysaccharides of Escherichia coli by CdS quantum dots. J Mat Chem 21:13445–13451CrossRef

Guo D, Wu C, Jiang H, Li Q, Wang X, Chen B (2008) Synergistic cytotoxic effect of different sized ZnO nanoparticles and daunorubicin against leukemia cancer cells under UV irradiation. J Photochem Photobiol B 93:119–126CrossRef

Haff LA, Smirnov IP (1997) Single nucleotide polymorphism identification assays using a thermostable DNA polymerase and MALDI-TOF MS. Genome Res 7:378–388

Hazen KC, Theisz GW, Howell SA (1999) Chronic urinary tract infection due to Candida utilis. J Clin Microbio 37(24):824–827

Hirschey MD, Han YJ, Stucky SD, Butler AJ (2006) Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots. Bio Inorg Chem 11:663–669CrossRef

Hussain SM et al (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–983CrossRef

Jin JY et al (2008) Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Chem Res Toxicol 21:1871–1877CrossRef

Ke Y, Kailasa SK, Wu HF, Chen ZY (2010) High resolution detection of high mass proteins up to 80,000 Da via multifunctional CdS quantum dots in laser desorption/ionization mass. Talanta 83:178–184CrossRef

Kloepfer JA, Mielke RE, Nadeau JL (2005) Uptake of CdSe and CdSe/ZnS quantum dots into bacteria via Purine-dependent mechanisms. Appl Environ Microbiol 71:2548–2557CrossRef

Liu YQ, Liu Y, Tay JH (2004) Universal sample preparation method for characterization of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Microbiol Biotechnol 65:143–148CrossRef

Lovric J et al (2005) Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots. Mol Med 83:377–385CrossRef

Manikandan M, Pasic L, Kannan V (2009) Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology. Bioresour Technol 100:3107–3112CrossRef

Meunier F, Aoun M, Bitar N (1992) Chronic urinary tract infection due to Candida utilis. Clin Infect Dis 14:120–125CrossRef

Nguyen DN, Becker GW, Riggin RM (1995) Protein mass spectrometry: applications to analytical biotechnology. J Chromatogr A 705:21–45CrossRef

Posteraro B, Sanguinetti M, D’Amore G, Masucci L, Morace G, Fadda G (1999) Molecular and epidemiological characterization of vaginal Saccharomyces cerevisiae isolates. J Clin Microbiol 37:2230–2235

Roepstorff P (2002) MALDI-TOF mass spectrometry in protein chemistry. Exs 88:81–97

Ross P, Hall LA, Smirnov IP, Haff L (1998) High level multiplex genotyping by MALDI-TOF mass spectrometry. Nat Biotechnol 16:1347–1351CrossRef

Schneider R et al (2009) The exposure of bacteria to CdTe-core quantum dots: the importance of surface chemistry on cytotoxicity. Nanotechnology 20:225101 (1–10)CrossRef

Service RF (2004) Nanotechnology grows up. Science 304:1732–1734CrossRef

Sheng G, Yu H, Wang C (2006) Extraction of extracellular polymeric substances from the photosynthetic bacterium Rodopseudomonas acidophila. Appl Microbial Cell Physiol 73:204–210

Stewart PS, Costerton JW (2001) Antibiotic resistance of bacteria in biofilms. Biofilm exopolysaccharides: a strong and sticky framework. Lancet 358:135–138CrossRef

Sutherland IW (2001) Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147:3–9

Voura EB, Jaiswal JK, Mattoussi H, Simon SM (2004) Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat Med 10:993–998CrossRef

Vuong C et al (2004) Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cell Microbiol 6:269–275CrossRef

Wang JJ, Sanderson BJ, Wang H (2007) Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells. Mutatgen Res 628:99–106CrossRef

Welham KJ, Domin MA, Scannell DE, Cohen E, Ashton DS (1998) The Characterisation of micro-organisms by MALDI/TOF mass spectrometry. Rapid Commun Mass Spectrom 12:176–180CrossRef

Yang H, Liu C, Yang D, Zhang H, Xi Z (2008) Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells. J Appl Toxicol 29:69–78CrossRef

Yu WW, Qu L, Guo W, Peng X (2003) Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals. Materials 15:2854–2860

Zaluzec EJ, Gage DA, Watson JT (1995) Matrix-assisted laser desorption ionization mass spectrometry: applications in peptide and protein characterization. Protein Expr Purif 6:109–123CrossRef

Title: Probing the fungicidal property of CdS quantum dots on Saccharomyces cerevisiae and Candida utilis using MALDI-MS
Authors: Muthu Manikandan
Hui-Fen Wu
Publication date: 01-07-2013
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 7/2013
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1728-9

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