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Published in: Colloid and Polymer Science 4/2008

01-04-2008 | Original Contribution

Structural and dynamical characterization of poly-gamma-glutamic acid-based cross-linked nanoparticles

Authors: Judit Éva Fleischer Radu, Levente Novak, John F. Hartmann, Neda Beheshti, Anna-Lena Kjøniksen, Bo Nyström, János Borbély

Published in: Colloid and Polymer Science | Issue 4/2008

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Abstract

This work describes the formation of water-soluble hydrophilic nanoparticles from biosynthetic poly-γ-glutamic acid (PGA). Nanoparticles were formed by cross-linking using 2,2′-(ethylenedioxy) diethylamine in the presence of water-soluble carbodiimide. The structure was determined by nuclear magnetic resonance spectroscopy and the particle size by transmission electron microscopy (TEM), size exclusion chromatography (SEC), and dynamic light-scattering (DLS) measurements. The results from TEM, SEC, and DLS reveal that the particle size depends on the ratio of cross-linking. Particle size values measured by TEM were between 20 and 90 nm. Formation of cross-linked nanoparticles results in a dramatic viscosity drop compared to the viscosity of the corresponding solution of the parent PGA. The viscosity and DLS experiments disclose an intriguing interplay between intrachain and interchain cross-linking of the polymer chains, depending on the cross-linker density and polymer concentration. The SEC measurements show that the retention time of the major portion of particles increase because of the higher cross-linking ratio. At moderate cross-linker concentration, intramolecular cross-linking is the dominant process, whereas at higher cross-linker densities, the interpolymer cross-linking plays an important role. As a result, large clusters are also formed.

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Metadata
Title
Structural and dynamical characterization of poly-gamma-glutamic acid-based cross-linked nanoparticles
Authors
Judit Éva Fleischer Radu
Levente Novak
John F. Hartmann
Neda Beheshti
Anna-Lena Kjøniksen
Bo Nyström
János Borbély
Publication date
01-04-2008
Publisher
Springer-Verlag
Published in
Colloid and Polymer Science / Issue 4/2008
Print ISSN: 0303-402X
Electronic ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-007-1776-8

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