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Journal of Nanoparticle Research

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01-06-2010 | Research Paper

The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages

Authors: Lanxia Liu, Yuanyuan Bai, Chunni Song, Dunwan Zhu, Liping Song, Hailing Zhang, Xia Dong, Xigang Leng

Published in: Journal of Nanoparticle Research | Issue 5/2010

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Abstract

It has been demonstrated that incorporation of arginine moieties into chitosan significantly elevates the transgenic efficacy of the chitosan. However, little is known about the impact of arginine-modified chitosan on the function of macrophages, which play a vitally important role in the inflammatory response of the body to foreign substances, especially particulate substances. This study was designed to investigate the impact of arginine-modified chitosan/DNA nanoparticles on the function of the murine macrophage through observation of phagocytic activity and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12, and TNF-α). Results showed that both chitosan/DNA nanoparticles and arginine-modified chitosan/DNA nanoparticles, containing 20 μg/mL DNA, were internalized by almost all the macrophages in contact. This led to no significant changes, compared to the non-exposure group, in production of cytokines and phagocytic activity of the macrophages 24 h post co-incubation, whereas exposure to LPS induced obviously elevated cytokine production and phagocytic activity, suggesting that incorporation of arginine moieties into chitosan does not have a negative impact on the function of the macrophages.

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Title: The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages
Authors: Lanxia Liu
Yuanyuan Bai
Chunni Song
Dunwan Zhu
Liping Song
Hailing Zhang
Xia Dong
Xigang Leng
Publication date: 01-06-2010
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 5/2010
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-009-9722-y

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