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Journal of Nanoparticle Research

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01-06-2010 | Research paper

Toxicity of zinc oxide nanoparticles to zebrafish embryo: a physicochemical study of toxicity mechanism

Authors: Wei Bai, Zhiyong Zhang, Wenjing Tian, Xiao He, Yuhui Ma, Yuliang Zhao, Zhifang Chai

Published in: Journal of Nanoparticle Research | Issue 5/2010

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Abstract

The biological impact of engineered nanomaterials released into the aquatic environment is a major concern. In this work, the properties of ZnO nanoparticles (nano-ZnO, 30 nm) were characterized in a water suspension (E3 medium), and a zebrafish 96-h post fertilization (hpf) embryo–larval test was performed to assess the toxicity of nano-ZnO suspension. Nano-ZnO was found to readily form aggregates with different sizes; small aggregates (142.4–517.7 nm) were still suspended in E3 medium, but large aggregates (>1 μm) quickly deposited on the bottom of 24-well plates; nano-ZnO was partially dissolved to Zn species (Zn_(dis)) in E3 medium. In the nano-ZnO suspension, small aggregates, Zn_(dis), and large aggregates might jointly exert influence on the development of zebrafish embryos. The embryo toxicity test revealed that nano-ZnO killed zebrafish embryos (50 and 100 mg/L), retarded the embryo hatching (1–25 mg/L), reduced the body length of larvae, and caused tail malformation after the 96 hpf exposure. Zn_(dis) only partially contributed to the toxicity of nano-ZnO. This research highlights the need to further investigate the ecotoxicity of nano-ZnO in the water environment.

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Title: Toxicity of zinc oxide nanoparticles to zebrafish embryo: a physicochemical study of toxicity mechanism
Authors: Wei Bai
Zhiyong Zhang
Wenjing Tian
Xiao He
Yuhui Ma
Yuliang Zhao
Zhifang Chai
Publication date: 01-06-2010
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 5/2010
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-009-9740-9

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