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Colloid and Polymer Science
Published in: Colloid and Polymer Science 10/2015

01-10-2015 | Original Contribution

Tunable immobilization of protein in anionic spherical polyelectrolyte brushes as observed by small-angle X-ray scattering

Authors: Weihua Wang, Li Li, Haoya Han, Yuchuan Tian, Zhiming Zhou, Xuhong Guo

Published in: Colloid and Polymer Science | Issue 10/2015

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Abstract

Tunable immobilization of bovine serum albumins (BSA) onto anionic spherical polyelectrolyte brushes (SPB) by changing BSA concentration, pH, and ionic strength was mainly observed by small-angle X-ray scattering (SAXS). Change of the BSA amount immobilized in SPB can be determined by SAXS which was confirmed by UV spectroscopy, and SAXS is the unique method to “see” the distribution of BSA in SPB. More BSA entered into brush layer upon increasing the protein concentration or decreasing the ionic strength of solutions. When pH increased from 3 to 5 (around the isoelectric point of BSA 4.9), more BSA came into the brush inner layer, while the proteins partly moved to the outer layer when pH continued to increase. After pH was higher than 7, most of BSA were desorbed from SPB. SAXS is proved to be a powerful tool to monitor the tunable immobilization and distribution of proteins in SPB.
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Metadata
Title
Tunable immobilization of protein in anionic spherical polyelectrolyte brushes as observed by small-angle X-ray scattering
Authors
Weihua Wang
Li Li
Haoya Han
Yuchuan Tian
Zhiming Zhou
Xuhong Guo
Publication date
01-10-2015
Publisher
Springer Berlin Heidelberg
Published in
Colloid and Polymer Science / Issue 10/2015
Print ISSN: 0303-402X
Electronic ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-015-3684-7

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