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Cellulose

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01-06-2014 | Original Paper

Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose

Authors: Hongming Lou, Haifeng Zhou, Xiuli Li, Mengxia Wang, J. Y. Zhu, Xueqing Qiu

Published in: Cellulose | Issue 3/2014

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Abstract

The effects of lignosulfonate (LS) on enzymatic saccharification of pure cellulose were studied. Four fractions of LS with different molecular weight (MW) prepared by ultrafiltration of a commercial LS were applied at different loadings to enzymatic hydrolysis of Whatman paper under different pH. Using LS fractions with low MW and high degree of sulfonation can enhance enzymatic cellulose saccharification despite LS can bind to cellulase nonproductively. The enhancing effect varies with LS properties, its loading, and hydrolysis pH. Inhibitive effect on cellulose saccharification was also observed using LS with large MW and low degree of sulfonation. The concept of “LS-cellulase aggregate stabilized and enhanced cellulase binding” was proposed to explain the observed enhancement of cellulose saccharification. The concept was demonstrated by the linear correlation between the measured amount of bound cellulase and saccharification efficiency with and without LS of different MW in a range of pH.

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Bozell JJ, Petersen GR (2010) Technology development for the production of biobased products from biorefinery carbohydrates—the US Department of Energy’s “top 10” revisited. Green Chem 12(4):539–554CrossRef

Bradford M (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254CrossRef

Castanon M, Wilke CR (1981) Effects of the surfactant Tween 80 on Enzymatic hydrolysis of Newspaper. Biotechnol Bioeng 13:1365–1372CrossRef

Eriksson T, Borjesson J, Tjerneld F (2002) Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzym Microbial Technol 31(3):353–364CrossRef

Goldemberg J (2007) Ethanol for a sustainable energy future. Science 315:808–810CrossRef

Haynes CA, Sliwinsky E, Norde W (1994) Structural and electrostatic properties of globular proteins at a polystyrene-water interface. J Colloid Interface Sci 164(2):394–409CrossRef

Helle SS, Duff SJB, Cooper DG (1993) Effect of surfactants on cellulose hydrolysis. Biotechnol Bioeng 42:611–617CrossRef

Henrissat B (1994) Cellulases and their interaction with cellulose. Cellulose 1(3):169–196CrossRef

Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807CrossRef

Igarashi K, Uchihashi T, Koivula A, Wada M, Kimura S, Okamoto T, Penttilä M, Ando T, Samejima M (2011) Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science 333(6047):1279–1282CrossRef

Kim MH, Lee SB, Ryu DDY, Reese ET (1982) Surface deactivation of cellulase and its prevention. Enzym Microbial Technol 4(2):99–103CrossRef

Lan TQ, Lou H, Zhu JY (2013) Enzymatic saccharification of lignocelluloses should be conducted at elevated pH 5.2–6.2. Bioenerg Res 6(2):476–485CrossRef

Lehtio J, Sugiyama J, Gustavsson M, Fransson L, Linder M, Teeri TT (2003) The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules. Proc Natl Acad Sci USA 100(2):484–489CrossRef

Leu SY, Zhu JY (2013) Substrate-related factors affecting enzymatic saccharification of lignocelluloses: our recent understanding. Bioenerg Res 6(2):405–415CrossRef

Leu S-Y, Gleisner R, Zhu JY, Sessions J, Marrs G (2013) Robust enzymatic saccharification of a Douglas-fir forest harvest residue by SPORL. Biomass Bioenergy 59:393–401CrossRef

Li J, Li S, Fan C, Yan Z (2012) The mechanism of poly(ethylene glycol) 4000 effect on enzymatic hydrolysis of lignocellulose. Colloids Surf B 89(1):203–210CrossRef

Liu H, Zhu JY (2010) Eliminating inhibition of enzymatic hydrolysis by lignosulfonate in unwashed sulfite-pretreated aspen using metal salts. Bioresour Technol 101(23):9120–9127CrossRef

Liu H, Zhu JY, Fu S (2010) Effects of lignin-metal complexation on enzymatic hydrolysis of cellulose. J Agric Food Chem 58:7233–7238CrossRef

Liu H, Zhu JY, Chai XS (2011) In situ, rapid, and temporally resolved measurements of cellulase adsorption onto lignocellulosic substrates by UV-vis spectrophotometry. Langmuir 27(1):272–278CrossRef

Lou H, Zhu JY, Lan TQ, Lai H, Qiu X (2013) pH-induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses. ChemSusChem 6(5):919–927CrossRef

Nakagame S, Chandra RP, Kadla JF, Saddler JN (2011a) Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin. Biotechnol Bioeng 108(3):538–548CrossRef

Nakagame S, Chandra RP, Kadla JF, Saddler JN (2011b) The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on the enzymatic hydrolysis of cellulose. Bioresour Technol 102(6):4507–4517CrossRef

Ooshima H, Sakata M, Harano Y (1986) Enhancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol Bioeng 28:1727–1734CrossRef

Ouyang X, Deng Y, Qian Y, Zhang P, Qiu X (2011) Adsorption characteristics of lignosulfonates in salt-free and salt-added aqueous solutions. Biomacromolecules 12(9):3313–3320CrossRef

Rollin JA, Zhu Z, Sathitsuksanoh N, Zhang Y-HP (2011) Increasing cellulose accessibility is more important than removing lignin: a comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia. Biotechnol Bioeng 108(1):22–30CrossRef

Shiiba H, Hayashi S, Yui T (2013) Molecular dynamics study of carbohydrate binding module mutants of fungal cellobiohydrolases. Carbohydr Res 374:96–102CrossRef

Várnai A, Siika-Aho M, Viikari L (2013) Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs. Biotechnol Biofuels 6(1)

Wang QQ, He Z, Zhu Z, Zhang Y-HP, Ni Y, Luo XL, Zhu JY (2012) Evaluations of cellulose accessibilities of lignocelluloses by solute exclusion and protein adsorption techniques. Biotechnol Bioeng 109(2):381–389CrossRef

Wang Z, Zhu JY, Fu Y, Qin M, Shao Z, Jiang J, Yang F (2013a) Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin. Biotechnol Biofuels 6:156CrossRef

Wang ZJ, Lan TQ, Zhu JY (2013b) Lignosulfonate and elevated pH can enhance enzymatic saccharification of lignocelluloses. Biotechnol Biofuels 6:9CrossRef

Zhou H, Lou H, Yang D, Zhu JY, Qiu X (2013a) Lignosulfonate to enhance enzymatic saccharification of lignocelluloses: Role of molecular weight and substrate lignin. Ind Eng Chem Res 52(25):8464–8470CrossRef

Zhou H, Zhu JY, Luo X, Leu S-Y, Wu X, Gleisner R, Dien BS, Hector RE, Yang D, Qiu X, Horn E, Negron J (2013b) Bioconversion of beetle-killed lodgepole pine using SPORL: Process scale-up design, lignin coproduct, and high solids fermentation without detoxification. Ind Eng Chem Res 52(45):16057–16065CrossRef

Zhu JY, Zhuang XS (2012) Conceptual net energy output for biofuel production from lignocellulosic biomass through biorefining. Prog Energy Combust Sci 38(4):583–589CrossRef

Zhu JY, Pan XJ, Wang GS, Gleisner R (2009) Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresour Technol 100(8):2411–2418CrossRef

Zhu JY, Gleisner R, Scott CT, Luo XL, Tian S (2011) High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: a comparison between SPORL and dilute acid pretreatments. Bioresour Technol 102(19):8921–8929CrossRef

Title: Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose
Authors: Hongming Lou
Haifeng Zhou
Xiuli Li
Mengxia Wang
J. Y. Zhu
Xueqing Qiu
Publication date: 01-06-2014
Publisher: Springer Netherlands
Published in: Cellulose / Issue 3/2014
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-014-0237-z

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