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Microsystem Technologies

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03-02-2018 | Technical Paper

A generic model for diffusive dynamics of the substrate and fluorescein tagged enzyme in microfluidic platform

Authors: Ece Yildiz-Ozturk, Mesut Yucel, Ozlem Yesil-Celiktas

Published in: Microsystem Technologies | Issue 7/2018

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Abstract

The aim of this study was to develop a model that describes enzymatic conversion in a microfluidic system along with convective and diffusive transport of substrate/product within the microchannels. Hence, a 1-D partial differential equation model based on Fick’s Law was employed in order to describe the substrate/product concentration distributions within the system. Enzyme immobilized in PEO-inclusive/non-inclusive TEOS-based hydrogels were tagged with fluorescein and examined under confocal laser scanning microscopy to evaluate the distribution of the enzyme. The image processing analysis demonstrated that 88.2 ± 5.9% of the PEO-inclusive gel fragment surface (pixelated) area was occupied by the enzyme-dye complex with an emission density of 29.92 ± 1.07. Subsequent to ensuring the homogeneity throughout the microchannel, the effects of flow rate and the distance for a range of inlet substrate concentrations were considered in regards to various substrate concentrations and conversion rates. The developed model provided a quantification for the conversion of substrate into products and enhanced understanding of the transport phenomena in the hydrogel.

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Title: A generic model for diffusive dynamics of the substrate and fluorescein tagged enzyme in microfluidic platform
Authors: Ece Yildiz-Ozturk
Mesut Yucel
Ozlem Yesil-Celiktas
Publication date: 03-02-2018
Publisher: Springer Berlin Heidelberg
Published in: Microsystem Technologies / Issue 7/2018
Print ISSN: 0946-7076
Electronic ISSN: 1432-1858
DOI: https://doi.org/10.1007/s00542-018-3746-0

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