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Journal of Nanoparticle Research

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01-12-2015 | Research Paper

Comparative synthesis and antimicrobial action of silver nanoparticles and silver nitrate

Authors: Dina A. Mosselhy, Mohamed Abd El-Aziz, Magdy Hanna, Mohamed A. Ahmed, Mona M. Husien, Qingling Feng

Published in: Journal of Nanoparticle Research | Issue 12/2015

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Abstract

The high wave of antibiotic bacterial resistance has addressed an importance for administration of different antibacterial agents, as silver nanoparticles (Ag NPs). However, many investigators still suffer conflict in the mechanistic antimicrobial action of Ag NPs and Ag⁺ ions. In this regard, our study investigated the comparative antimicrobial action of different sizes of Ag NPs as 8 (nAg1) and 29 (nAg2) nm, in comparison with silver nitrate (AgNO₃) against five different bacterial species; Aeromonas hydrophila (A. hydrophila), Pseudomonas putida (Ps. putida), Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Bacillus subtilis (B. subtilis) using agar diffusion assay and minimum inhibitory concentration (MIC). The key role of the size of nanomaterials was detected, as the smaller Ag NPs (nAg1) showed more antimicrobial action than the larger particles. Transmission electron microscopy (TEM) studies demonstrated the different mechanistic antibacterial actions of Ag NPs and AgNO₃. The effect of combining Ag NPs with antibiotics was also investigated. Synergistic effect of combining Ag NPs with ampicillin was detected against S. aureus, in a size-dependent manner as well. To summarize, our results point towards the major role played by the size of Ag NPs in their antimicrobial effects and the different toxic mechanisms of actions induced by Ag NPs and AgNO₃.

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Title: Comparative synthesis and antimicrobial action of silver nanoparticles and silver nitrate
Authors: Dina A. Mosselhy
Mohamed Abd El-Aziz
Magdy Hanna
Mohamed A. Ahmed
Mona M. Husien
Qingling Feng
Publication date: 01-12-2015
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 12/2015
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-015-3279-8

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