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Cellulose
Published in: Cellulose 2/2013

01-04-2013 | Original Paper

Mechanical deconstruction of lignocellulose cell walls and their enzymatic saccharification

Authors: Ingrid C. Hoeger, Sandeep S. Nair, Arthur J. Ragauskas, Yulin Deng, Orlando J. Rojas, J. Y. Zhu

Published in: Cellulose | Issue 2/2013

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Abstract

Laboratory mechanical softwood pulps (MSP) and commercial bleached softwood kraft pulps (BSKP) were mechanically fibrillated by stone grinding with a SuperMassColloider®. The extent of fibrillation was evaluated by SEM imaging, water retention value (WRV) and cellulase adsorption. Both lignin content and mechanical treatment significantly affected deconstruction and enzymatic saccharification of fibrillated MSP and BSKP. Fibrillation of MSP and BSKP cell walls occurs rapidly and then levels off; further fibrillation has only limited effect on cell wall breakdown as measured by water retention value and cellulase adsorption. Complete (100 %) saccharification can be achieved at cellulase loading of 5 FPU/g glucan for BSKP after only 15 min fibrillation with energy input of 0.69 MJ/kg. However, the presence of lignin in MSP affects the extent of fibrillation producing fibrils mainly above 1 μm. Lignin binds nonproductively to cellulases and blocks cellulose thereby reducing its accessibility. As a result, the cellulose saccharification efficiency of MSP fibrils (6 h of fibrillation, energy input of 13.33 MJ/kg) was only 55 % at same cellulase loading of 5 FPU/g glucan.
previous article Degradation of TEMPO-oxidized cellulose fibers and nanofibrils by crude cellulase
next article Mechanical, thermal, and moisture absorption properties of nano-clay reinforced nano-cellulose biocomposites

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Metadata
Title
Mechanical deconstruction of lignocellulose cell walls and their enzymatic saccharification
Authors
Ingrid C. Hoeger
Sandeep S. Nair
Arthur J. Ragauskas
Yulin Deng
Orlando J. Rojas
J. Y. Zhu
Publication date
01-04-2013
Publisher
Springer Netherlands
Published in
Cellulose / Issue 2/2013
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-013-9867-9

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