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28-04-2020 | Original Paper

Extraction of pectin from albedo of lemon peels for preparation of tissue engineering scaffolds

Authors: Didem Demir, Seda Ceylan, Dilek Göktürk, Nimet Bölgen

Published in: Polymer Bulletin | Issue 4/2021

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Abstract

Pectin is a type of anionic polysaccharide naturally found in a number of fruits and vegetables. Although pectin is widely used for food industry, its biomedical applications such as wound dressing, drug delivery and cancer targeting have also been investigated. In our study, we combined extracted pectin (from albedo of lemon peels) with chitosan (as a natural polymer) to synthesize chitosan/pectin cryogels. The extracted pectin was subjected to qualitative and quantitative analyses. Chitosan/pectin spongy supermacroporous cryogels were produced by cryogelation method at different combinations (100:0, 80:20, 60:40 and 40:60, w/w). Polyelectrolyte interactions between pectin and chitosan and crosslinking of chitosan with glutaraldehyde were verified by using FTIR. The porosity, swelling ratio, degradation behaviors and mechanical properties of cryogels were determined. SEM analysis demonstrated the pore morphology and average pore diameters of cryogels. After all analysis, 40:60 chitosan/pectin cryogel was selected for cytotoxicity studies. Glioblastoma (U-87 MG) cell line was used to evaluate the in vitro cytotoxicity of scaffolds. MTT assay and SEM analyses demonstrated the scaffolds were nontoxic, and supported cell attachment and viability.

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Title: Extraction of pectin from albedo of lemon peels for preparation of tissue engineering scaffolds
Authors: Didem Demir
Seda Ceylan
Dilek Göktürk
Nimet Bölgen
Publication date: 28-04-2020
Publisher: Springer Berlin Heidelberg
Published in: Polymer Bulletin / Issue 4/2021
Print ISSN: 0170-0839
Electronic ISSN: 1436-2449
DOI: https://doi.org/10.1007/s00289-020-03208-1

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