Top

Published in:

01-07-2022 | Research paper

Physicochemical and biological characterization of oxidized multi-walled carbon nanotubes on HepG2 liver cells

Authors: Jorge A. Uribe-Calderon, Cielo G. Poot-Bote, José M. Cervantes-Uc, Elda L. Pacheco-Pantoja, Ileana Echevarría-Machado, Nayeli Rodríguez-Fuentes

Published in: Journal of Nanoparticle Research | Issue 7/2022

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Functionalization of multi-walled carbon nanotubes (MWCNTs) is a growing area in the field of materials with biological applications; due that their surface characteristics could determine their biocompatibility and therapeutic uses. This research is focused on the evaluation of the effect of oxidized MWCNTs on the cell cultures of a hepatic carcinoma cell line (HepG2). Pristine MWCNTs (p-MWCNT) with an external diameter of 8–15 nm and a length in the range of 10–50 μm were oxidized with (a) nitric acid/hydrogen peroxide, (b) nitric acid, and (c) nitric acid/sulfuric acid solutions, and their physicochemical properties and biological effects were examined. The findings demonstrated that MWCNT oxidation produced different surface moieties and structural changes depending on the oxidation process, in particular, the oxidation with nitric acid/sulfuric acid generates a high grade of cell toxicity compared to the other types of oxidized MWCNTs. Interestingly, the p-MWCNTs exhibited slight cytotoxic and genotoxic effects but without affecting cell viability, which requires further analysis. The results open the possibility of using oxidized MWCNT with nitric acid/sulfuric acid to promote cytotoxic effects on cancer cells, as well as to explore different oxidative methods in medical applications.

previous article In vitro and in vivo safety profile assessment of graphene oxide decorated with different concentrations of magnetite

next article Hydrogen and humidity sensing characteristics of Nafion, Nafion/graphene, and Nafion/carbon nanotube resistivity sensors

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Available only for authorised users

Aimonen K, Imani M, Hartikainen M et al (2022) Surface functionalization and size modulate the formation of reactive oxygen species and genotoxic effects of cellulose nanofibrils. Part Fibre Toxicol 19(1):19. https://doi.org/10.1186/s12989-022-00460-3CrossRef

Auría-Soro C, Nesma T, Juanes-Velasco P et al (2019) Interactions of nanoparticles and biosystems: microenvironment of nanoparticles and biomolecules in nanomedicine. Nanomaterials (Basel) 9(10). https://doi.org/10.3390/nano9101365

Avila-Orta CA, Cruz V, Neira-Velazquez MG et al (2009) Surface modification of carbon nanotubes with ethylene glycol plasma. Carbon 47(8):1916–1921. https://doi.org/10.1016/j.carbon.2009.02.033CrossRef

Aviles F, Cauich J, Moo-Tah L et al (2009) Evaluation of mild acid oxidation treatments for MWCNT functionalization. Carbon 47(13):2970–2975. https://doi.org/10.1016/j.carbon.2009.06.044CrossRef

Bokobza L, Zhang J (2012) Raman spectroscopic characterization of multiwall carbon nanotubes and of composites. eXPRESS Polym Lett:601–608. https://doi.org/10.3144/expresspolymlett.2012.63

Bottini M, Bruckner S, Nika K et al (2006) Multi-walled carbon nanotubes induce T lymphocyte apoptosis. Toxicol Lett 160(2):121–126. https://doi.org/10.1016/j.toxlet.2005.06.020CrossRef

Bussy C, Methven L, Kostarelos K (2013) Hemotoxicity of carbon nanotubes. Adv Drug Deliv Rev 65(15):2127–2134. https://doi.org/10.1016/j.addr.2013.10.008CrossRef

Carbone M, Valentini F, Caminiti R et al (2010) Are PEI-coated SWCNTs conjugated with hepatitis A virus? A chemical study with SEM, Z-potential, EDXD and RT-PCR. Biomed Mater 5(3):35001. https://doi.org/10.1088/1748-6041/5/3/035001CrossRef

Chatterjee N, Yang J, Dahye Y et al (2017) Differential crosstalk between global DNA methylation and metabolomics associated with cell type specific stress response by pristine and functionalized MWCNT. Biomaterials 115:167–180. https://doi.org/10.1016/j.biomaterials.2016.11.005CrossRef

Chowdhry A, Kaur J, Khatri M et al (2019) Characterization of functionalized multiwalled carbon nanotubes and comparison of their cellular toxicity between HEK 293 cells and zebra fish in vivo. Heliyon 5(10):e02605. https://doi.org/10.1016/j.heliyon.2019.e02605CrossRef

Cirillo G, Vittorio O, Kunhardt D et al (2019) Combining carbon nanotubes and chitosan for the vectorization of methotrexate to lung cancer cells. Materials 12(18):2889. https://doi.org/10.3390/ma12182889CrossRef

Eigler S, Hirsch A (2014) Chemistry with graphene and graphene oxide. Challenges for synthetic chemists, Angew. Chemie Int Ed (53): 7720-7738. https://doi.org/10.1002/anie.201402780

Fraczek-Szczypta A, Menaszek E, Syeda TB et al (2012) Effect of MWCNT surface and chemical modification on in vitro cellular response. J Nanopart Res 14(10):1181. https://doi.org/10.1007/s11051-012-1181-1CrossRef

Gangopadhyay M, Mukhopadhyay SK, Gayathri S et al (2016) Fluorene–morpholine-based organic nanoparticles: lysosome-targeted pH-triggered two-photon photodynamic therapy with fluorescence switch on–off. J Mater Chem B 10(4):1862–1868. https://doi.org/10.1039/c5tb02563CrossRef

Gavello D, Fenoglio I, Fubini B et al (2013) Inhibition of catecholamine secretion by iron-rich and iron-deprived multiwalled carbon nanotubes in chromaffin cells. Neurotoxicology 39:84–94. https://doi.org/10.1016/j.neuro.2013.08.008CrossRef

Ghasemvand F, Biazar E, Tavakolifard S et al (2016) Synthesis and evaluation of multi-wall carbon nanotube-paclitaxel complex as an anti-cancer agent. Gastroenterol Hepatol Bed Bench 9(3):197–204

Guo J, Zhang X, Li Q et al (2007) Biodistribution of functionalized multiwall carbon nanotubes in mice. Nucl Med Biol 34(5):579–583. https://doi.org/10.1016/j.nucmedbio.2007.03.003CrossRef

Guzman-Mendoza J, Montes-Fonseca SL, Ramos-Martínez E et al (2020) Safe administration of carbon nanotubes by intravenous pathway in BALB/c mice. Nanomaterials (Basel) 10(2). https://doi.org/10.3390/nano10020400

Hindman B, Ma Q (2019) Carbon nanotubes and crystalline silica stimulate robust ROS production, inflammasome activation, and IL-1beta secretion in macrophages to induce myofibroblast transformation. Arch Toxicol 93(4):887–907. https://doi.org/10.1007/s00204-019-02411-yCrossRef

Hong-Bin Z, Guo-Dong L, Zhen-Hua Z (2002) Raman spectra of MWCNTs and MWCNT-based H2-adsorbing system. Carbon 40:2429–2436. https://doi.org/10.1016/S0008-6223(02)00148-3CrossRef

Hui Y, Liu C, Yang D et al (2009) Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition. J Appl Toxicol 29:69–78. https://doi.org/10.1002/jat.1385CrossRef

ISO, I.O.f.S. (2009) Biological evaluation of medical devices —Part 5: Tests for in vitro cytotoxicityx

Jacobsen NR, Møller P, Clausen PA et al (2017) Biodistribution of carbon nanotubes in animal models. Basic Clin Pharmacol Toxicol 121(Suppl 3):30–43. https://doi.org/10.1111/bcpt.12705CrossRef

Jia G, Wang H, Yan L et al (2005) Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene. Environ Sci Technol 39(5):1378–1383. https://doi.org/10.1021/es048729lCrossRef

Kamble RV, Kamble RV, Bhinge SD et al (2021) In vitro targeting and selective killing of mcf-7 and colo320dm cells by 5-fluorouracil anchored to carboxylated SWCNTs and MWCNTs. J Mater Sci Mater Med 32(6):71. https://doi.org/10.1007/s10856-021-06540-8CrossRef

Kato T, Totsuka Y, Ishino K, Matsumoto Y, Tada Y, Nakae D, Yagi T (2013) Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems. Nanotoxicology 7(4):452–461. https://doi.org/10.3109/17435390.2012.674571CrossRef

Khan HA, Kishore U, Alsulami HM et al (2021) Pro-apoptotic and immunotherapeutic effects of carbon nanotubes functionalized with recombinant human surfactant protein d on leukemic cells. Int J Mol Sci 22(19). https://doi.org/10.3390/ijms221910445

Khazaei S, Abdul-Hamid R, Mohd-Esa N et al (2017) Promotion of HepG2 cell apoptosis by flower of Allium atroviolaceum and the mechanism of action. BMC Complement Altern Med. 17:104–108. https://doi.org/10.1186/s12906-017-1594-6CrossRef

Kobayashi N, Izumi H, Morimoto Y (2017) Review of toxicity studies of carbon nanotubes. J Occup Health 59(5):394–407. https://doi.org/10.1539/joh.17-0089-RACrossRef

Kolosnjaj-Tabi J, Hartman KB, Boudjemaa S et al (2010) In vivo behavior of large doses of ultrashort and full-length single-walled carbon nanotubes after oral and intraperitoneal administration to Swiss mice. ACS Nano 4(3):1481–1492. https://doi.org/10.1021/nn901573wCrossRef

Lee DK, Jeon S, Jeong J et al (2020) Potential role of soluble metal impurities in the acute lung inflammogenicity of multi-walled carbon nanotubes. Nanomaterials (Basel):10(2). https://doi.org/10.3390/nano10020379

Li Y, Cao J (2018) The impact of multi-walled carbon nanotubes (MWCNTs) on macrophages: contribution of MWCNT characteristics. Sci China Life Sci 61(11):1333–1351. https://doi.org/10.1007/s11427-017-9242-3CrossRef

Li S, He P, Dong J, Guo Z, Dai L (2005) DNA-directed self-assembling of carbon nanotubes. J Am Chem Soc 127:14–15. https://doi.org/10.1021/ja0446045CrossRef

Liu X, Nakamura F (2020) Mechanotransduction, nanotechnology, and nanomedicine. J Biomed Res 35(4):284–293. https://doi.org/10.7555/JBR.34.20200063CrossRef

Locken-Castilla A, Pacheco-Pantoja EL, Rodríguez-Brito F et al (2019) Smoking index, lifestyle factors, and genomic instability assessed by single-cell gel electrophoresis: a cross-sectional study in subjects from Yucatan, Mexico. Clin Epigenetics 11(1):150. https://doi.org/10.1186/s13148-019-0745-7CrossRef

Magrez A, Kasas S, Salicio V et al (2006) Cellular toxicity of carbon-based nanomaterials. Nano Lett 6(6):1121–1125. https://doi.org/10.1021/nl060162eCrossRef

Mohajerani A, Burnett L, Smith JV et al (2019) Nanoparticles in construction materials and other applications, and implications of nanoparticle use. Materials (Basel) 12(19). https://doi.org/10.3390/ma12193052

Montanheiro T, Cristóvan F, Machado J et al (2015) Effect of MWCNT functionalization on thermal and electrical properties of PHBV/MWCNT nanocomposites. J Mater Res 30(1):55–65. https://doi.org/10.1557/jmr.2014.303CrossRef

Moulder JF, Chastain J, King RC (1995) Handbook of X-Ray Photoelectron Spectroscopy: A Reference Book of Standard Spectra for Identification and Interpretation of XPS Data. Physical Electronics Division, Perkin-Elmer Corp, Eden Prairie, Minn

Muller J, Huaux F, Moreau N et al (2005) Respiratory toxicity of multi-wall carbon nanotubes. Toxicol Appl Pharmacol 207(3):221–231. https://doi.org/10.1016/j.taap.2005.01.008CrossRef

National Toxicology Program (2019 ) Toxicity studies of 1020 Long Multiwalled Carbon Nanotubes (L-MWNT-1020) administered by inhalation to Sprague Dawley (Hsd:Sprague Dawley SD) rats and B6C3F1/N mice. Toxic Rep Ser (94). https://doi.org/10.22427/NTP-TOX-94

Nazib A, Zaine I, Napiah NAM (2014) Study on dispersion and characterization of functionalized MWCNTs prepared by wet oxidation. Appl Mech Mater 661:8–13. https://doi.org/10.4028/www.scientific.net/AMM.661.8CrossRef

Patlolla AK, Hussain SM, Schlager JJ et al (2010) Comparative study of the clastogenicity of functionalized and nonfunctionalized multiwalled carbon nanotubes in bone marrow cells of Swiss-Webster mice. Environ Toxicol 25(6):608–621. https://doi.org/10.1002/tox.20621CrossRef

Patra JK, Das G, Fraceto LF et al (2018) Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology 16(1):71. https://doi.org/10.1186/s12951-018-0392-8CrossRef

Pratap Singh B, Choudhary V, Teotia S et al (2015) Solvent free, efficient, industrially viable, fast dispersion process based amine modified MWCNT reinforced epoxy composites of superior mechanical properties. Adv Mater Lett 6:104–113. https://doi.org/10.5185/amlett.2015.5612CrossRef

Renero-Lecuna C, Iturrioz-Rodríguez N, González-Lavado E, Padín-González E, Navarro-Palomares E, Valdivia-Fernández L, García-Hevia L, Fanarraga ML, González-Legarreta L (2019) Effect of size, shape, and composition on the interaction of different nanomaterials with HeLa cells. J Nanomater 1:1–11. https://doi.org/10.1155/2019/7518482CrossRef

Riviere JE (2009) Pharmacokinetics of nanomaterials: an overview of carbon nanotubes, fullerenes and quantum dots. Wiley Interdiscip Rev Nanomed Nanobiotechnol 1(1):26–34. https://doi.org/10.1002/wnan.24CrossRef

Shang Y, Hasan MK, Ahammed GJ et al (2019) Applications of nanotechnology in plant growth and crop protection: a review. Molecules 24(14). https://doi.org/10.3390/molecules24142558

Shen J, Huang W, Wu L et al (2007) Study on amino-functionalized multiwalled carbon nanotubes. Mater Sci Eng A 464(1-2):151–156. https://doi.org/10.1016/j.msea.2007.02.091CrossRef

Shen J, Yang D, Chen MY et al (2021) Effects of length and chemical modification on the activation of vascular endothelial cells induced by multi walled carbon nanotubes. Beijing Da Xue Xue Bao Yi Xue Ban 53(3):439–446. https://doi.org/10.19723/j.issn.1671-167X.2021.03.001CrossRef

Shinohara N, Nakazato T, Ohkawa K et al (2016) Long-term retention of pristine multi-walled carbon nanotubes in rat lungs after intratracheal instillation. J Appl Toxicol 36(4):501–509. https://doi.org/10.1002/jat.3271CrossRef

Singh NP, McCoy MT, Tice RR et al (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175(1):184–191. https://doi.org/10.1016/0014-4827(88)90265-0CrossRef

Sinis SI, Hatzoglou C, Gourgoulianis KI et al (2018) Carbon nanotubes and other engineered nanoparticles induced pathophysiology on mesothelial cells and mesothelial membranes. Front Physiol 29(9):295. https://doi.org/10.3389/fphys.2018.00295CrossRef

Srivastava R, Pant AB, Kashyap MP et al (2011) Multi-walled carbon nanotubes induce oxidative stress and apoptosis in human lung cancer cell line-A549. Nanotoxicology 5(2):195–207. https://doi.org/10.3109/17435390.2010.503944CrossRef

International Organization for Standardization (2017) Biological evaluation of medical devices — Part 4: Selection of tests for interactions with blood (ISO 10993-4:2017, pp. 1–69

Ursini C, Cavallo D, Fresegna AM et al (2012) Comparative cyto-genotoxicity assessment of functionalized and pristine multiwalled carbon nanotubes on human lung epithelial cells. Toxicol in Vitro 26(6):831–840. https://doi.org/10.1016/j.tiv.2012.05.001CrossRef

Ursini CL, Cavallo D, Fresegna AM et al (2014) Differences in cytotoxic, genotoxic, and inflammatory response of bronchial and alveolar human lung epithelial cells to pristine and COOH-functionalized multiwalled carbon nanotubes. Biomed Res Int 2014:359506. https://doi.org/10.1155/2014/359506CrossRef

Ursini CL, Maiello R, Ciervo A et al (2016) Evaluation of uptake, cytotoxicity and inflammatory effects in respiratory cells exposed to pristine and -OH and -COOH functionalized multi-wall carbon nanotubes. J Appl Toxicol 36(3):394–403. https://doi.org/10.1002/jat.3228CrossRef

Vennerberg D, Hall R, Kessler MR (2014) Supercritical carbon dioxide-assisted silanization of multi-walled carbon nanotubes and their effect on the thermo-mechanical properties of epoxy nanocomposites. Polymer 55:4156–4163. https://doi.org/10.1016/j.polymer.2014.06.020CrossRef

Vincent-Crist B (1999) Oxides. In: Handbook of The Elements and Native Oxides. XPS International, Inc, pp 5–8

Yu J, Liu S, Wu B et al (2016a) Comparison of cytotoxicity and inhibition of membrane ABC transporters induced by MWCNTs with different length and functional groups. Environ Sci Technol 50(7):3985–3994. https://doi.org/10.1021/acs.est.5b05772CrossRef

Yu M, Chen R, Jia Z et al (2016b) MWCNTs induce ROS generation, ERK phosphorylation, and SOD-2 expression in human mesothelial cells. Int J Toxicol 35(1):17–26. https://doi.org/10.1177/1091581815591223CrossRef

Zhang L, Li Y, Qiao L et al (2015) Protective effects of hepatic stellate cells against cisplatin-induced apoptosis in human hepatoma G2 cells. Int J Oncol 47(2):632–640. https://doi.org/10.3892/ijo.2015.3024CrossRef

Zhang P, Teng J, Wang L (2020) Multiwalled carbon nanotubes inhibit cell migration and invasion by destroying actin cytoskeleton via mitochondrial dysfunction in ovarian cancer cells. Aging (Albany NY) 12(24):25294–25303. https://doi.org/10.18632/aging.104130CrossRef

Zhiyuan Z, Youwang H, Jianping L et al (2013) Multiple functionalization of multi-walled carbon nanotubes with carboxyl and amino groups. Appl Surf Sci 276:476–481. https://doi.org/10.1016/j.apsusc.2013.03.119CrossRef

Zhou L, Forman HJ, Ge Y, Lunec J (2017) Multi-walled carbon nanotubes: A cytotoxicity study in relation to functionalization, dose and dispersion. Toxicol In Vitro 42:292–298. https://doi.org/10.1016/j.tiv.2017.04.027CrossRef

Title: Physicochemical and biological characterization of oxidized multi-walled carbon nanotubes on HepG2 liver cells
Authors: Jorge A. Uribe-Calderon
Cielo G. Poot-Bote
José M. Cervantes-Uc
Elda L. Pacheco-Pantoja
Ileana Echevarría-Machado
Nayeli Rodríguez-Fuentes
Publication date: 01-07-2022
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 7/2022
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-022-05489-1

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 7/2022

Facile one-pot synthesis of bimodal-sized nickel nanoparticles in a solvent-directed reaction system

Catalytic activity of laser-synthesized CrOx/Al2O3 nanocatalysts with different particle sizes in isobutane dehydrogenation

Synthetic 2D tellurium nanosheets with intense TE wave polarization absorption by employing the PVD method

Synthesis and characterization of calcium oxide nanoparticles for CO2 capture

Magnesium oxide nanofabrication from magnesium chloride salt by thermal plasma method: effects of quenching and concentration

Effects of laser penetration depth and temperature on the stability of afatinib-loaded gold nanoparticles: an optical limiting study

Premium Partners