Skip to main content
main-content
Top

Hint

Swipe to navigate through the articles of this issue

Published in: Cellulose 4/2022

24-01-2022 | Original Research

Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study

Authors: Zahra Sadat Sajadi-Javan, Jaleh Varshosaz, Mina Mirian, Maziar Manshaei, Atousa Aminzadeh

Published in: Cellulose | Issue 4/2022

Login to get access
share
SHARE

Abstract

In-situ forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25–1.5 w/v% PG and 1–7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs. In vitro release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.

Graphical abstract

To get access to this content you need the following product:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 50.000 Bücher
  • über 380 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




Testen Sie jetzt 15 Tage kostenlos.

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 69.000 Bücher
  • über 500 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Testen Sie jetzt 15 Tage kostenlos.

Literature
go back to reference Abbasi S (2019) Persian gum (Amygdalus scoparia Spach). In: Razavi SMA (ed) Emerging natural hydrocolloids: rheology and functions. John Wiley & Sons Ltd, Hoboken, pp 273–298 CrossRef Abbasi S (2019) Persian gum (Amygdalus scoparia Spach). In: Razavi SMA (ed) Emerging natural hydrocolloids: rheology and functions. John Wiley & Sons Ltd, Hoboken, pp 273–298 CrossRef
go back to reference Abbasi S, Mohammadi S, Rahimi S (2011) Partial substitution of gelatin with Persian gum and use of Olibanum in production of functional pastille. Biosyst Eng 42:121–131 Abbasi S, Mohammadi S, Rahimi S (2011) Partial substitution of gelatin with Persian gum and use of Olibanum in production of functional pastille. Biosyst Eng 42:121–131
go back to reference Kumar A, Rao KM, Han SS (2017) Development of sodium alginate-xanthan gum based nanocomposite scaffolds reinforced with cellulose nanocrystals and halloysite nanotubes. Polym Test 63:214–225 CrossRef Kumar A, Rao KM, Han SS (2017) Development of sodium alginate-xanthan gum based nanocomposite scaffolds reinforced with cellulose nanocrystals and halloysite nanotubes. Polym Test 63:214–225 CrossRef
Metadata
Title
Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study
Authors
Zahra Sadat Sajadi-Javan
Jaleh Varshosaz
Mina Mirian
Maziar Manshaei
Atousa Aminzadeh
Publication date
24-01-2022
Publisher
Springer Netherlands
Published in
Cellulose / Issue 4/2022
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-021-04383-8