Assess to Alleviating Effect of Salicylate On Boron Stress in Nigella damascena by Utilizing Biplot Analysis

The purpose of this research was to investigate whether sodium salicylate (NaSA) treatment could ameliorate the stress caused by B toxicity in Nigella damascena. In the study, the effects of untreated control, 1 mM NaSA (NaSA), 4 ppm B (B4), 8 ppm B (B8), B4 + NaSA, and B8 + NaSA on grain yield, oil content, and fatty acid composition of N. damascena were examined. The B4 and B8 applications reduced nearly all of the studied characteristics in N. damascena. Plant seed yield decreased by 45% and 55%, In B4- and B8-applications, respectively. Compared to the B4 and B8 applications, the B4 + NaSA and B8 + NaSA applications increased grain yield by 16% and 17%, respectively. NaSA application yielded the highest both β‑elemene and α‑selinene ratios. Application-Trait-biplot (AT-biplot) explained 76.1% of total variation. Based on the positive relationship between B and NaSA applications, the properties in this investigation, which mostly included fatty acids, were divided into five groups: (I) palmitic acid, total-saturated-fatty-acid (TSFA), eicosadienoic acid, linolenic acid, and total-unsaturated-fatty-acid (TUFA), (II) monounsaturated-fatty-acid (MUFA), myristic, stearic, and oleic acids, (III) cis-oleic acid, oil content and thousand-kernel-weight (TKW), (IV) plant grain yield, β‑elemene, α‑selinene, and TUFA/TSFA ratio and linoleic acid, (V) polyunsaturated-fatty-acid (PUFA). As seen in the biplot analysis, compared to the B4 application, the B4 + NaSA application brought the majority of examined features closer to values of control application. As a consequence, it was discovered that the B‑toxicity in N. damascena species may be alleviated by NaSA application, and the data collected under various stress circumstances can be examined visually using a biplot.