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01.03.2014 | Research Paper

A cellular uptake and cytotoxicity properties study of gallic acid-loaded mesoporous silica nanoparticles on Caco-2 cells

verfasst von: Ladan Rashidi, Ebrahim Vasheghani-Farahani, Masoud Soleimani, Amir Atashi, Khosrow Rostami, Fariba Gangi, Masoud Fallahpour, Mohammad Taher Tahouri

Erschienen in: Journal of Nanoparticle Research | Ausgabe 3/2014

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Abstract

In this study, the effects of intracellular delivery of various concentrations of gallic acid (GA) as a semistable antioxidant, gallic acid-loaded mesoporous silica nanoparticles (MSNs-GA), and cellular uptake of nanoparticles into Caco-2 cells were investigated. MSNs were synthesized and loaded with GA, then characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, N₂ adsorption isotherms, X-ray diffraction, and thermal gravimetric analysis. The cytotoxicity of MSNs and MSNs-GA at low and high concentrations were studied by means of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test and flow cytometry. MSNs did not show significant toxicity in various concentrations (0–500 μg/ml) on Caco-2 cells. For MSNs-GA, cell viability was reduced as a function of incubation time and different concentrations of nanoparticles. The in vitro GA release from MSNs-GA exhibited the same antitumor properties as free GA on Caco-2 cells. Flow cytometry results confirmed those obtained using MTT assay. TEM and fluorescent microscopy confirmed the internalization of MSNs by Caco-2 cells through nonspecific cellular uptake. MSNs can easily internalize into Caco-2 cells without deleterious effects on cell viability. The cell viability of Caco-2 cells was affected during MSNs-GA uptake. MSNs could be designed as suitable nanocarriers for antioxidants delivery.

Vorheriger Artikel Acoustic wave in a suspension of magnetic nanoparticle with sodium oleate coating

Nächster Artikel Spectroscopic investigation of colloidal CdS quantum dots–methylene blue hybrid associates

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Titel: A cellular uptake and cytotoxicity properties study of gallic acid-loaded mesoporous silica nanoparticles on Caco-2 cells
verfasst von: Ladan Rashidi
Ebrahim Vasheghani-Farahani
Masoud Soleimani
Amir Atashi
Khosrow Rostami
Fariba Gangi
Masoud Fallahpour
Mohammad Taher Tahouri
Publikationsdatum: 01.03.2014
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 3/2014
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2285-6

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