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2021 | OriginalPaper | Buchkapitel

6. Antibacterial and Anticancer Activity of Biologically Synthesized Gold Nanoparticles

verfasst von : Azra Parveen, Hadeel Salih Mahdi, Ameer Azam

Erschienen in: Emerging Trends in Nanotechnology

Verlag: Springer Singapore

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Abstract

The rapid developments in the field of nanotechnology have spurred interest in many metal nanoparticles. Among many other uses, these nanoparticles are increasingly providing helpful ways to tackle-resistant bacteria. This research was aimed to identify the effect of biologically synthesized Au-NPs on bacteria Pseudomonas aeruginosa and Staphylococcus aureus and on the cancer cell lines. The antibacterial effect of Au-NPs against P. aeruginosa and S. aureus was studied by scanning electron microscopy (SEM) analysis. The bacterial culture (P. aeruginosa and S. aureus) was exposed to sublethal concentrations of Au-NPs (25 µg/mL) and incubated at 37 °C for 12 h. Antibacterial activity was observed to increase as Au-NPs increased in concentration from 25 to 100 µg/mL. The antimicrobial efficacy of Au-NPs was fairly different in Gram-positive and Gram-negative bacteria. The rigid structure of Gram-positive bacteria is likely responsible for this as it contains high peptidoglycan content that provides superior protective ability and enhances pathogenicity. To determine the effect of Au-NPs on the cell lines used in this study, the cell lines were grown in RPMI 1640 medium containing 10% fetal bovine serum and 2 mM L-glutamine. The study shows that the treatment with Au-NPs was notably effective against the proliferation of MDA-MB-231 cancer cells in a dose-dependent method. The cytotoxicity assays revealed the concentration-dependent cytotoxic effects of Au-NPs in range of 0–80 µg/ml. The results obtained confirm the strong activity of biologically synthesized Au-NPs against bacteria and cancer cell lines.

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Titel: Antibacterial and Anticancer Activity of Biologically Synthesized Gold Nanoparticles
verfasst von: Azra Parveen
Hadeel Salih Mahdi
Ameer Azam
Verlag: Springer Singapore
Buch: Emerging Trends in Nanotechnology
Print ISBN: 978-981-15-9903-3

Electronic ISBN: 978-981-15-9904-0

Copyright-Jahr: 2021
DOI: https://doi.org/10.1007/978-981-15-9904-0_6

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