nach oben

Erschienen in:

2013 | OriginalPaper | Buchkapitel

Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin_1A Receptor

verfasst von : Md. Jafurulla, Amitabha Chattopadhyay

Erschienen in: Fluorescent Methods to Study Biological Membranes

Verlag: Springer Berlin Heidelberg

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

G protein-coupled receptors (GPCRs) are the largest class of molecules involved in signal transduction across membranes and represent major targets in the development of novel drug candidates in all clinical areas. Recent advances in understanding of the nonrandom distribution of GPCRs, G proteins, and effector molecules have given rise to new challenges and complexities in cellular signaling by GPCRs. In this article, we provide specific examples on the application of quantitative fluorescence microscopic approaches to monitor organization and dynamics of the serotonin_1A receptor (a GPCR) in live cells. This assumes broader relevance due to the emerging theme that GPCR function depends on its organization and dynamics. We envisage that with progress in understanding of receptor organization and dynamics, our knowledge of GPCR function would improve considerably, thereby enabling to design better therapeutic strategies to combat diseases related to malfunctioning of GPCRs.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Vorheriges Kapitel New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization

Nächstes Kapitel TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy

Albizu L, Cottet M, Kralikova M, Stoev S, Seyer R, Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A, Javitch J, Trinquet E, Manning M, Pin JP, Mouillac B, Durroux T (2010) Time-resolved FRET between GPCR ligands reveals oligomers in native tissues. Nat Chem Biol 6:587–594CrossRef

Banerjee P, Joo JB, Buse JT, Dawson G (1995) Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem Phys Lipids 77:65–78CrossRef

Benda A, Benes M, Marecek V, Lhotsky A, Hermens WT, Hof M (2003) How to determine diffusion coefficients in planar phospholipid systems by confocal fluorescence correlation spectroscopy. Langmuir 19:4120–4126CrossRef

Blier P, Ward NM (2003) Is there a role for 5-HT_1A agonists in the treatment of depression? Biol Psychiatry 53:193–203CrossRef

Broder CC, Dimitrov DS (1996) HIV and the 7-transmembrane domain receptors. Pathobiology 64:171–179CrossRef

Brown DA, Rose JK (1992) Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68:533–544CrossRef

Calvert PD, Govardovskii VI, Krasnoperova N, Anderson RE, Lem J, Makino CL (2001) Membrane protein diffusion sets the speed of rod phototransduction. Nature 411:90–94CrossRef

Chamberlain LH (2004) Detergents as tools for the purification and classification of lipid rafts. FEBS Lett 559:1–5CrossRef

dos Remedios CG, Chhabra D, Kekic M, Dedova IV, Tsubakihara M, Berry DA, Nosworthy NJ (2003) Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol Rev 83:433–473

10.

Eggeling C, Ringemann C, Medda R, Schwarzmann G, Sandhoff K, Polyakova S, Belov VN, Hein B, von Middendorff C, Schönle A, Hell SW (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457:1159–1163CrossRef

11.

Ganguly S, Chattopadhyay A (2010) Cholesterol depletion mimics the effect of cytoskeletal destabilization on membrane dynamics of the serotonin_1A receptor: a zFCS study. Biophys J 99:1397–1407CrossRef

12.

Ganguly S, Clayton AHA, Chattopadhyay A (2011) Organization of higher-order oligomers of the serotonin_1A receptor explored utilizing homo-FRET in live cells. Biophys J 100:361–368CrossRef

13.

Ganguly S, Paila YD, Chattopadhyay A (2011) Metabolic depletion of sphingolipids enhances the mobility of the human serotonin_1A receptor. Biochem Biophys Res Commun 411:180–184CrossRef

14.

Ganguly S, Pucadyil TJ, Chattopadhyay A (2008) Actin cytoskeleton-dependent dynamics of the human serotonin_1A receptor correlates with receptor signaling. Biophys J 95:451–463CrossRef

15.

Ganguly S, Saxena R, Chattopadhyay A (2011) Reorganization of the actin cytoskeleton upon G-protein coupled receptor signaling. Biochim Biophys Acta 1808:1921–1929CrossRef

16.

Ganguly S, Singh P, Manoharlal R, Prasad R, Chattopadhyay A (2009) Differential dynamics of membrane proteins in yeast. Biochem Biophys Res Commun 387:661–665CrossRef

17.

Gardier AM (2009) Mutant mouse models and antidepressant drug research: focus on serotonin and brain-derived neurotrophic factor. Behav Pharmacol 20:18–32CrossRef

18.

Gether U (2000) Uncovering molecular mechanisms involved in activation of G protein-coupled receptors. Endocr Rev 21:90–113CrossRef

19.

Giepmans BNG, Adams SR, Ellisman MH, Tsien RY (2006) The fluorescent toolbox for assessing protein location and function. Science 312:217–224CrossRef

20.

González-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, López-Giménez JF, Zhou M, Okawa Y, Callodo LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, Sealfon SC (2008) Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature 452:93–97CrossRef

21.

Griebel G (1999) 5-HT_1A receptor blockers as potential drug candidates for the treatment of anxiety disorders. Drug News Perspect 12:484–490CrossRef

22.

Haldar S, Chattopadhyay A (2009) The green journey. J Fluoresc 19:1–2CrossRef

23.

Haldar S, Chattopadhyay A (2009) Green fluorescent protein: a molecular lantern that illuminates the cellular interior. J Biosci 34:169–172CrossRef

24.

Harding PJ, Attrill H, Boehringer J, Ross S, Wadhams GH, Smith E, Armitage JP, Watts A (2009) Constitutive dimerization of the G-protein coupled receptor, neurotensin receptor 1, reconstituted into phospholipid bilayers. Biophys J 96:964–973CrossRef

25.

Harrison T, Samuel BU, Akompong T, Hamm H, Mohandas N, Lomasney JW, Haldar K (2003) Erythrocyte G protein-coupled receptor signaling in malarial infection. Science 301:1734–1736CrossRef

26.

Haustein E, Schwille P (2007) Fluorescence correlation spectroscopy: novel variations of an established technique. Annu Rev Biophys Biomol Struct 36:151–169CrossRef

27.

He H-T, Marguet D (2011) Detecting nanodomains in living cell membrane by fluorescence correlation spectroscopy. Annu Rev Phys Chem 62:417–436CrossRef

28.

Heerklotz H (2002) Triton promotes domain formation in lipid raft mixtures. Biophys J 83:2693–2701CrossRef

29.

Heilker R, Wolff M, Tautermann CS, Bieler M (2009) G-protein coupled receptor-focused drug discovery using a target class platform approach. Drug Discov Today 14:231–240CrossRef

30.

Hess ST, Huang S, Heikal AA, Webb WW (2002) Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry 41:697–705CrossRef

31.

Hooper NM (1999) Detergent-insoluble glycosphingolipid/cholesterol-rich membrane domains, lipid rafts and caveolae. Mol Membr Biol 16:145–156CrossRef

32.

Howe AK (2004) Regulation of actin-based cell migration by cAMP/PKA. Biochim Biophys Acta 1692:159–174CrossRef

33.

Huang C, Hepler JR, Chen LT, Gilman AG, Anderson RG, Mumby SM (1997) Organization of G proteins and adenylyl cyclase at the plasma membrane. Mol Biol Cell 8:2365–2378

34.

Hui KL, Wang C, Grooman B, Wayt J, Upadhyaya A (2012) Membrane dynamics correlate with formation of signaling clusters during cell spreading. Biophys J 102:1524–1533CrossRef

35.

Humpolícková J, Gielen E, Benda A, Fagulova V, Vercammen J, vandeVen M, Hof M, Ameloot M, Engelborghs Y (2006) Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy. Biophys J 91:L23–L25CrossRef

36.

Hur E-M, Kim KT (2002) G protein-coupled receptor signalling and cross-talk: achieving rapidity and specificity. Cell Signal 14:397–405CrossRef

37.

Jacobson K, Mouritsen OG, Anderson RGW (2007) Lipid rafts: at a crossroad between cell biology and physics. Nat Cell Biol 9:7–14CrossRef

38.

Jafurulla M, Pucadyil TJ, Chattopadhyay A (2008) Effect of sphingomylinase treatment on ligand binding activity of human serotonin_1A receptors. Biochim Biophys Acta 1778:2022–2025CrossRef

39.

James JR, Oliveira MI, Carmo AM, Iaboni A, Davis SJ (2006) A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer. Nat Methods 3:1001–1006CrossRef

40.

Janmey PA (1998) The cytoskeleton and cell signaling: component localization and mechanical coupling. Physiol Rev 78:763–781

41.

Jans DA, Peters R, Jans P, Fahrenholz F (1991) Vasopressin V₂ receptor mobile fraction and ligand-dependent adenylate cyclase activity are directly correlated in LLC-PK1 renal epithelial cells. J Cell Biol 114:53–60CrossRef

42.

Kalipatnapu S, Chattopadhyay A (2004) A GFP fluorescence based approach to determine detergent insolubility of the human serotonin_1A receptor. FEBS Lett 576:455–460CrossRef

43.

Kalipatnapu S, Chattopadhyay A (2005) Membrane organization of the human serotonin_1A receptor monitored by detergent insolubility using GFP fluorescence. Mol Membr Biol 22:539–547CrossRef

44.

Kalipatnapu S, Chattopadhyay A (2007) Membrane organization and function of the serotonin_1A receptor. Cell Mol Neurobiol 27:1097–1116CrossRef

45.

Kalipatnapu S, Chattopadhyay A (2007) Membrane organization of the serotonin_1A receptor monitored by a detergent-free approach. Cell Mol Neurobiol 27:463–474CrossRef

46.

Kalipatnapu S, Pucadyil TJ, Chattopadhyay A (2007) Membrane organization and dynamics of the serotonin_1A receptor monitored using fluorescence microscopic approaches. In: Chattopadhyay A (ed) Serotonin receptors in neurobiology, new frontiers in neuroscience series. CRC Press, Boca Raton, pp 41–60

47.

Karnovsky MJ, Kleinfeld AM, Hoover RL, Klausner RD (1982) The concept of lipid domains in membranes. J Cell Biol 94:1–6CrossRef

48.

Kasai RS, Suzuki KGN, Prossnitz ER, Koyama-Honda I, Nakada C, Fujiwara TK, Kusumi A (2011) Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging. J Cell Biol 192:463–480CrossRef

49.

Kusumi A, Nakada C, Ritchie K, Murase K, Suzuki K, Murakoshi H, Kasai RS, Kondo J, Fujiwara T (2005) Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules. Annu Rev Biophys Biomol Struct 34:351–378CrossRef

50.

Lajoie P, Goetz JG, Dennis JW, Nabi IR (2009) Lattices, rafts and scaffolds: domain regulation of receptor signaling at the plasma membrane. J Cell Biol 185:381–385CrossRef

51.

Lenne P-F, Wawrezinieck L, Conchonaud F, Wurtz O, Boned A, Guo X-J, Rigneault H, He H-T, Marguet D (2006) Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork. EMBO J 25:3245–3256CrossRef

52.

Lidke DS, Nagy P, Barisas BG, Heintzmann R, Post JN, Lidke KA, Clayton AH, Arndt-Jovin DJ, Jovin TM (2003) Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET). Biochem Soc Trans 31:1020–1027CrossRef

53.

Lin SH, Civelli O (2004) Orphan G protein-coupled receptors: targets for new therapeutic interventions. Ann Med 36:204–214CrossRef

54.

Lingwood D, Simons K (2010) Lipid rafts as a membrane-organizing principle. Science 327:46–50CrossRef

55.

Lohse MJ (2010) Dimerization in GPCR mobility and signaling. Curr Opin Pharmacol 10:53–58CrossRef

56.

Lohse MJ, Nikolaev VO, Hein P, Hoffmann C, Vilardaga J-P, Bünemann M (2008) Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors. Trends Pharmacol Sci 29:159–165CrossRef

57.

Meyer BH, Segura JM, Martinez KL, Hovius R, George N, Johnsson K, Vogel H (2006) FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci USA 103:2138–2143CrossRef

58.

Milligan G (2007) G protein-coupled receptor dimerisation: molecular basis and relevance to function. Biochim Biophys Acta 1768:825–835CrossRef

59.

Mukherjee S, Maxfield FR (2004) Membrane domains. Annu Rev Cell Dev Biol 20:839–866CrossRef

60.

Müller CP, Carey RJ, Huston JP, De Souza Silva MA (2007) Serotonin and psychostimulant addiction: focus on 5-HT_1A-receptors. Prog Neurobiol 81:133–178CrossRef

61.

Neubig RR (1994) Membrane organization in G-protein mechanisms. FASEB J 8:939–946

62.

Oldham WM, Hamm HE (2006) Structural basis of function in heterotrimeric G proteins. Q Rev Biophys 39:117–166CrossRef

63.

Ostrom RS, Insel PA (2004) The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: implications for molecular pharmacology. Br J Pharmacol 143:235–245CrossRef

64.

Paila YD, Chattopadhyay A (2010) Membrane cholesterol in the function and organization of G-protein coupled receptors. Subcell Biochem 51:439–466CrossRef

65.

Paila YD, Ganguly S, Chattopadhyay A (2010) Metabolic depletion of sphingolipids impairs ligand binding and signaling of human serotonin_1A receptors. Biochemistry 49:2389–2397CrossRef

66.

Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A (2011) Oligomerization of the serotonin_1A receptor in live cells: a time-resolved fluorescence anisotropy approach. J Phys Chem B 115:11439–11447CrossRef

67.

Paila YD, Murty MRVS, Vairamani M, Chattopadhyay A (2008) Signaling by the human serotonin_1A receptor is impaired in cellular model of Smith-Lemli-Opitz Syndrome. Biochim Biophys Acta 1778:1508–1516CrossRef

68.

Perez DM (2003) The evolutionarily triumphant G-protein-coupled receptor. Mol Pharmacol 63:1202–1205CrossRef

69.

Pierce KL, Premont RT, Lefkowitz RJ (2002) Seven-transmembrane receptors. Nat Rev Mol Cell Biol 3:639–650CrossRef

70.

Pucadyil TJ, Chattopadhyay A (2004) Cholesterol modulates ligand binding and G-protein coupling to serotonin_1A receptors from bovine hippocampus. Biochim Biophys Acta 1663:188–200CrossRef

71.

Pucadyil TJ, Chattopadhyay A (2006) Role of cholesterol in the function and organization of G-protein coupled receptors. Prog Lipid Res 45:295–333CrossRef

72.

Pucadyil TJ, Chattopadhyay A (2007) Cholesterol depletion induces dynamic confinement of the G-protein coupled serotonin_1A receptor in the plasma membrane of living cells. Biochim Biophys Acta 1768:655–668CrossRef

73.

Pucadyil TJ, Chattopadhyay A (2007) Cholesterol: a potential therapeutic target in Leishmania infection? Trends Parasitol 23:49–53CrossRef

74.

Pucadyil TJ, Kalipatnapu S, Chattopadhyay A (2005) Membrane organization and dynamics of the G-protein-coupled serotonin_1A receptor monitored using fluorescence-based approaches. J Fluoresc 15:785–796CrossRef

75.

Pucadyil TJ, Kalipatnapu S, Chattopadhyay A (2005) The serotonin_1A receptor: A representative member of the serotonin receptor family. Cell Mol Neurobiol 25:553–580CrossRef

76.

Pucadyil TJ, Kalipatnapu S, Harikumar KG, Rangaraj N, Karnik SS, Chattopadhyay A (2004) G-protein-dependent cell surface dynamics of the human serotonin_1A receptor tagged to yellow fluorescent protein. Biochemistry 43:15852–15862CrossRef

77.

Raymond JR, Mukhin YV, Gettys TW, Garnovskaya MN (1999) The recombinant 5-HT_1A receptor: G protein coupling and signalling pathways. Br J Pharmacol 127:1751–1764CrossRef

78.

Renner U, Glebov K, Lang T, Papusheva E, Balakrishnan S, Keller B, Richter DW, Jahn R, Ponimaskin E (2007) Localization of the mouse 5-hydroxytryptamine_1A receptor in lipid microdomains depends on its palmitoylation and is involved in receptor-mediated signaling. Mol Pharmacol 72:502–513CrossRef

79.

Riethmüller J, Riehle A, Grassmé H, Gulbins E (2006) Membrane rafts in host-pathogen interactions. Biochim Biophys Acta 1758:2139–2147CrossRef

80.

Rosenbaum DM, Rasmussen SGF, Kobilka BK (2009) The structure and function of G-protein-coupled receptors. Nature 459:356–363CrossRef

81.

Runnels LW, Scarlata SF (1995) Theory and application of fluorescence homotransfer to melittin oligomerization. Biophys J 69:1569–1583CrossRef

82.

Sahu S, Saxena R, Chattopadhyay A (2012) Cholesterol depletion modulates detergent resistant fraction of human serotonin_1A receptors. Mol Membr Biol (in press)

83.

Saxena R, Chattopadhyay A (2011) Membrane organization and dynamics of the serotonin_1A receptor in live cells. J Neurochem 116:726–733CrossRef

84.

Schlyer S, Horuk R (2006) I want a new drug: G-protein-coupled receptors in drug development. Drug Discov Today 11:481–493CrossRef

85.

Schroeder RJ, Ahmed SN, Zhu Y, London E, Brown DA (1998) Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositol-anchored proteins by promoting the formation of detergent-insoluble ordered membrane domains. J Biol Chem 273:1150–1157CrossRef

86.

Shanti K, Chattopadhyay A (2000) A new paradigm in the functioning of G-protein-coupled receptors. Curr Sci 79:402–403

87.

Shrivastava S, Pucadyil TJ, Paila YD, Ganguly S, Chattopadhyay A (2010) Chronic cholesterol depletion using statin impairs the function and dynamics of human serotonin_1A receptors. Biochemistry 49:5426–5435CrossRef

88.

Simons K, Toomre D (2000) Lipid rafts and signal transduction. Nat Rev Mol Cell Biol 1:31–39CrossRef

89.

Simons K, van Meer G (1988) Lipid sorting in epithelial cells. Biochemistry 27:6197–6202CrossRef

90.

Singh P, Chattopadhyay A (2012) Removal of sphingomyelin headgroup inhibits the ligand binding function of hippocampal serotonin_1A receptors. Biochem Biophys Res Commun 419:321–325CrossRef

91.

Terrillon S, Bouvier M (2004) Roles of G-protein-coupled receptor dimerization. EMBO Rep 5:30–34CrossRef

92.

Tramier M, Piolot T, Gautier I, Mignotte V, Coppey J, Kemnitz K, Durieux C, Coppey-Moisan M (2003) Homo-FRET versus hetero-FRET to probe homodimers in living cells. Methods Enzymol 360:580–597CrossRef

93.

Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509–544CrossRef

94.

Varma R, Mayor S (1998) GPI-anchored proteins are organized in submicron domains at the cell surface. Nature 394:798–801CrossRef

95.

Wawrezinieck L, Rigneault H, Marguet D, Lenne P-F (2005) Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization. Biophys J 89:4029–4042CrossRef

96.

Woehler A, Wlodarczyk J, Ponimaskin EG (2009) Specific oligomerization of the 5-HT1A receptor in the plasma membrane. Glycoconj J 26:749–756CrossRef

97.

Yeow EKL, Clayton AHA (2007) Enumeration of oligomerization states of membrane proteins in living cells by homo-FRET spectroscopy and microscopy: theory and application. Biophys J 92:3098–3104CrossRef

98.

Zhang Y, DeVries ME, Skolnick J (2006) Structure modeling of all identified G protein-coupled receptors in the human genome. PLoS Comput Biol 2:88–99CrossRef

99.

Zhou FC, Patel TD, Swartz D, Xu Y, Kelley MR (1999) Production and characterization of an anti-serotonin 1A receptor antibody which detects functional 5-HT_1A binding sites. Brain Res Mol Brain Res 69:186–201CrossRef

Titel: Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor
verfasst von: Md. Jafurulla
Amitabha Chattopadhyay
Verlag: Springer Berlin Heidelberg
Buch: Fluorescent Methods to Study Biological Membranes
Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright-Jahr: 2013
DOI: https://doi.org/10.1007/4243_2012_58

Premium Partner

Marktübersichten

Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.

Zur Marktübersicht