Introduction
Materials and methods
Location of soil sampling
Objects | Morphological composition |
---|---|
A | Ceramic construction materials, rubble, roofing paper, remnants of mortar, foamed concrete, foamed polystyrene |
B | Car tires, plastic containers from food products, plastic foil, metal elements, metallic foils, paper, metal containers of paint, glass bottles, elements of light bulbs and fluorescent tubes, medical packing and wrappings |
C | The waste of organic origin from households and cut grass as well as residue from garden maintenance |
Soil analysis
Physicochemical parameters of the soil
Microbial analysis
Biochemical measurements
Statistical analysis
Results and discussion
Physical and chemical characteristics of soils
Objects | pH H2O | pH KCl | Organic-C g kg−1
| Particle size fractions % | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Sand | Silt | Clay | ||||||||||
Min | Max | Min | Max | Min | Max | Min | Max | Min | Max | Min | Max | |
Control | 4.2 | 4.75 | 3.85 | 4.39 | 9.5 | 14.2 | 79.89 | 90.62 | 8.57 | 17.9 | 0.81 | 2.21 |
A | 6.74 | 8.56 | 6.54 | 8.02 | 2.3 | 4.10 | 80.5 | 90.25 | 8.81 | 18.65 | 0.85 | 1.71 |
B | 4.42 | 4.83 | 4.35 | 4.67 | 1.60 | 4.80 | 77.55 | 93.1 | 4.93 | 20.54 | 0.85 | 1.97 |
C | 6.98 | 7.34 | 6.85 | 7.30 | 10.8 | 26.5 | 79.98 | 91.25 | 7.37 | 17.67 | 0.98 | 2.35 |
Content of the selected heavy metals in soil
Parameters | Samples (n) | Min (mg kg−1) | Max (mg kg−1) | Mean (mg kg−1) | SD | CV % |
---|---|---|---|---|---|---|
Zn | ||||||
Control | 6 | 13.2 | 17.83 | 15.37 | 2.48 | 16.11 |
A | 10 | 16.8 | 25.5 | 20.93 | 4.39 | 20.96 |
B | 10 | 22.83 | 45.94 | 34.19 | 10.02 | 29.31 |
C | 10 | 54.83 | 276 | 165.83 | 127.09 | 76.64 |
Cu | ||||||
Control | 6 | 3.05 | 5.53 | 4.06 | 1.12 | 27.66 |
A | 10 | 2.30 | 5.68 | 4.11 | 1.51 | 36.60 |
B | 10 | 4.53 | 9.58 | 6.82 | 2.15 | 31.67 |
C | 10 | 18.55 | 108 | 47.84 | 41.80 | 87.39 |
Pb | ||||||
Control | 6 | 4.91 | 7.62 | 6.13 | 1.40 | 22.82 |
A | 10 | 0.20 | 4.12 | 2.02 | 1.98 | 97.81 |
B | 10 | 4.31 | 9.24 | 5.82 | 2.30 | 39.53 |
C | 10 | 4.84 | 21.48 | 15.80 | 7.83 | 49.54 |
Ni | ||||||
Control | 6 | 2.50 | 4.65 | 3.68 | 0.92 | 24.91 |
A | 10 | 3.50 | 4.18 | 4.03 | 0.42 | 10.42 |
B | 10 | 4.98 | 6.22 | 5.55 | 0.53 | 9.56 |
C | 10 | 7.03 | 14.25 | 12.80 | 3.99 | 31.25 |
Parameters | Samples (n) | Min (mg kg−1) | Max (mg kg−1) | Mean (mg kg−1) | SD | CV % |
---|---|---|---|---|---|---|
Zn | ||||||
Control | 6 | 2.07 | 2.13 | 2.10 | 0.03 | 1.69 |
A | 10 | 1.13 | 2.70 | 1.9 | 0.87 | 46.03 |
B | 10 | 9.99 | 21.82 | 15.82 | 6.28 | 39.71 |
C | 10 | 6.78 | 100.5 | 53.96 | 54.4 | 100.9 |
Cu | ||||||
Control | 6 | 0.35 | 0.40 | 0.38 | 0.02 | 6.55 |
A | 10 | 0.35 | 0.98 | 0.58 | 0.30 | 51.33 |
B | 10 | 3.71 | 7.00 | 5.32 | 1.72 | 32.46 |
C | 10 | 2.03 | 10.71 | 6.37 | 5.01 | 78.71 |
Pb | ||||||
Control | 6 | 0.36 | 1.97 | 0.87 | 0.75 | 85.75 |
A | 10 | 0.36 | 1.99 | 1.08 | 0.73 | 67.59 |
B | 10 | 0.39 | 2.84 | 1.42 | 1.11 | 77.96 |
C | 10 | 2.03 | 10.71 | 5.72 | 4.13 | 72.25 |
Ni | ||||||
Control | 6 | 0.13 | 1.98 | 0.79 | 0.84 | 107.2 |
A | 10 | 0.21 | 1.13 | 0.94 | 0.88 | 93.34 |
B | 10 | 1.34 | 28.45 | 12.6 | 12.8 | 102.0 |
C | 10 | 0.19 | 7.49 | 2.68 | 3.40 | 126.9 |
Variable | CF | CF in C
deg (%) | AF (%) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | A | B | C | Control | A | B | C | Control | A | B | C | |
Zn | 0.85 | 1.50 | 2.44 | 11.85 | 24.39 | 39.43 | 38.80 | 27.67 | 13.66 | 9.08 | 46.27 | 32.54 |
Cu | 1.02 | 1.03 | 1.71 | 11.96 | 29.12 | 27.11 | 27.12 | 27.94 | 9.35 | 14.11 | 78.00 | 13.31 |
Pb | 0.80 | 0.26 | 0.76 | 15.80 | 22.84 | 6.91 | 12.02 | 36.91 | 14.19 | 54.46 | 24.45 | 36.20 |
Ni | 0.92 | 1.01 | 1.39 | 3.20 | 26.40 | 26.56 | 22.06 | 7.48 | 21.47 | 23.32 | 22.91 | 20.94 |
C
deg
| 3.49 | 3.79 | 6.29 | 42.81 |
Microbiological parameters
Objects |
Escherichia coli (MPN g−1) | Total coliforms (MPN g−1) |
Salmonella spp. (MPN g−1) |
Enterococcus spp. (MPN g−1) |
---|---|---|---|---|
Control | n.d* | 1600.0 | n.d | 250.0 |
A | n.d | 1600.0 | n.d | n.d |
B | 9.5 | 1750.0 | n.d | n.d |
C | 96.0 | 3000.0 | 25.0 | 45.0 |
Enzyme activities
Parameters | Samples (n) | Min | Max | Mean | SD | CV % |
---|---|---|---|---|---|---|
DEH | ||||||
Control | 6 | 0.216 | 0.480 | 0.330 | 0.124 | 37.70 |
A | 10 | 0.096 | 0.144 | 0.120 | 0.019 | 16.32 |
B | 10 | 0.072 | 0.192 | 0.126 | 0.063 | 50.09 |
C | 10 | 2.136 | 8.304 | 5.202 | 3.540 | 68.06 |
CAT | ||||||
Control | 6 | 10.64 | 18.96 | 15.15 | 4.389 | 28.97 |
A | 10 | 6.285 | 15.85 | 11.82 | 4.754 | 40.22 |
B | 10 | 8.285 | 11.14 | 9.661 | 1.273 | 13.18 |
C | 10 | 40.85 | 91.41 | 66.00 | 27.28 | 41.33 |
AlP | ||||||
Control | 6 | 1.030 | 1.188 | 1.109 | 0.081 | 7.367 |
A | 10 | 0.548 | 0.859 | 0.693 | 0.161 | 23.28 |
B | 10 | 0.234 | 0.559 | 0.389 | 0.177 | 45.68 |
C | 10 | 1.353 | 1.990 | 1.657 | 0.340 | 20.51 |
AcP | ||||||
Control | 6 | 1.508 | 2.270 | 1.896 | 0.409 | 21.60 |
A | 10 | 0.408 | 1.065 | 0.723 | 0.337 | 46.71 |
B | 10 | 0.442 | 2.372 | 1.400 | 1.067 | 76.23 |
C | 10 | 1.771 | 2.552 | 2.139 | 0.412 | 19.25 |
Objects | DEH | CAT | AlP | AcP |
---|---|---|---|---|
A | −63.6 | −21.9 | −37.5 | −61.9 |
B | −61.8 | −36.2 | −64.9 | −26.2 |
C | 1475 | 335 | 49.5 | 12.8 |
Variables | Equation |
r
|
r
2
| |
---|---|---|---|---|
Dependent | Independent | |||
Total copper | Total organic carbon |
y = 7.0314 + 0.2073x
| 0.537 | 0.328 |
Total lead | Total organic carbon |
y = 3.4495 + 0.9184x
| 0.615 | 0.378 |
Total nickel | Total organic carbon |
y = −0.2062 + 1.6107x
| 0.705 | 0.497 |
Dehydrogenases | Available zinc |
y = −0.0166 + 0.0792x
| 0.949 | 0.902 |
Dehydrogenases | Total organic carbon |
y = −0.0827 + 0.1484x
| 0.524 | 0.275 |
Catalase | Total organic carbon |
y = 7.5574 + 1.7595x
| 0.627 | 0.393 |
Alkaline phosphatase | Total organic carbon |
y = 0.5524 + 0.0398x
| 0.734 | 0.538 |
Dehydrogenases | Total lead |
y = −1.361 + 0.3768x
| 0.891 | 0.793 |
Catalase | Total lead |
y = −2.1856 + 3.739x
| 0.892 | 0.796 |
Alkaline phosphatase | Total lead |
y = 0.5022 + 0.0618x
| 0.762 | 0.581 |
Acid phosphatase | Total lead |
y = 1.0221 + 0.0696x
| 0.568 | 0.323 |
Dehydrogenases | Total zinc |
y = −0.4209 + 0.0316x
| 0.984 | 0.973 |
Catalase | Total zinc |
y = 7.8495 + 0.3014x
| 0.950 | 0.903 |
Alkaline phosphatase | Total zinc |
y = 0.6927 + 0.0046x
| 0.742 | 0.552 |
Total coliforms | Total organic carbon |
y = 57.790x + 1392.9 | 0.709 | 0.502 |
E. coli
| Total organic carbon |
y = 2.355x − 4.0452 | 0.690 | 0.475 |
E. coli
| Total nickel |
y = 7.302x − 27.386 | 0.936 | 0.876 |
E. coli
| Total lead |
y = 4.176x − 10.907 | 0.819 | 0.670 |
Total coliforms | Total nickel |
y = 143.3x + 1053.2 | 0.770 | 0.593 |
Total coliforms | Total lead |
y = 91.43x + 1306.7 | 0.750 | 0.563 |
Salmonella spp. | Total nickel |
y = 2.0181x − 6.961 | 0.705 | 0.496 |
No and elements | Component matrix | ||
---|---|---|---|
PC1 | PC2 | PC3 | |
1. ZnDTPA
|
−0.888
| −0.106 | −0.046 |
2. CuDTPA
|
−0.756
| −0.153 | −0.054 |
3. PbDTPA
|
−0.924
| −0.017 | −0.052 |
4. NiDTPA
| −0.626 | −0.182 | −0.279 |
5. ZnTot
|
−0.938
| −0.029 | −0.040 |
6. CuTot
|
−0.909
| −0.137 | −0.222 |
7. PbTot
|
−0.929
| −0.161 | 0.156 |
8. NiTot
|
−0.899
| 0.145 | 0.027 |
9. Corg | −0.667 | −0.085 | 0.154 |
10. pH H2O | −0.317 |
0.768
| −0.418 |
11. pH KCl | −0.362 |
0.791
| −0.392 |
12. Sand | 0.095 | 0.411 |
0.807
|
13. Silt | −0.049 | −0.376 |
−0.805
|
14. Clay | −0.462 | −0.492 | −0.385 |
15. Dehydrogenases |
−0.962
| −0.002 | 0.021 |
16. Catalase |
−0.974
| 0.001 | 0.050 |
17. Alkaline phosphatase |
−0.816
| −0.113 | 0.223 |
18. Acid phosphatase |
−0.562
| −0.543 | 0.276 |
19. Escherichia coli
|
−0.836
| 0.262 | 0.231 |
20. Total coliforms |
−0.768
| 0.179 | 0.133 |
21. Salmonella
| −0.663 | 0.379 | 0.344 |
22. Enterococcus spp. | 0.174 | −0.581 | 0.508 |
Variation (%) |
52.34
| 12.7 | 11.55 |
Eigenvalue | 11.51 | 2.80 | 2.54 |