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Abstract
This chapter, which is divided into four blocks for a better comprehension by the reader, provides essential information about various chromatographic methodologies for the analysis of chlorophyll and carotenoid pigments responsible for the color of olive oil.
The first block is focused on the general aspects of chlorophyll and carotenoid pigments such as their distribution, location, structures, functions, and biological activities and, especially, the spectroscopic and chemical properties that one must know to carry out their identification.
In the second block, the authors carry out a critical description of the available current information on methodologies used for chromatographic analysis of pigments (chlorophylls and carotenoids) in virgin olive oil (VOO), including both those allowing the direct injection of diluted oil and those requiring an enrichment step using liquid-phase extraction (LPE) or solid-phase extraction (SPE).
The third block summarizes the current information about the presence of chlorophyll and carotenoid pigments in fruit and olive oil, besides their changes in profile and concentration associated with metabolism, or processing and storage conditions. The presence of exclusive pigments can be used as a marker of varietal VOO, and pigment ratios are suggested as chemical markers for determining the genuineness and correct processing. In addition, the changes in the pigment profile are shown to be a useful parameter to monitor the degradation, loss of freshness, or adulteration by colorants of VOO.
Finally, the fourth block describes the most recent researches related to kinetic studies of the thermodegradation of pigments, design of prediction mathematical models of the evolution of pigments over time, and future trends in the methods for the analysis of pigments.
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