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Erschienen in: Measurement Techniques 10/2022

12.03.2022

Color Calibration Slide for Digital Microscopy Systems

verfasst von: D. S. Makhov, G. R. Sagatelyan, A. V. Samorodov

Erschienen in: Measurement Techniques | Ausgabe 10/2022

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Abstract

The article is dedicated to the image standardization problem in digital microscopy. Color reproducibility in microscopic images is of great importance as it can lead to therapeutic errors in image interpretation. Color reproducibility in digital microscopy systems with various hardware can be ensured with color calibration using a special slide. The proposed requirements for color slide development are based on the analysis of the literature. A color slide prototype consisting of 97 fields of colored glass has been developed and manufactured. Color chromaticity coordinates in CIE XYZ color space have been calculated from experimentally determined spectral transmitted radiation distributions. A comparative study of the proposed color slide prototype and existing color slide gamuts according to the most frequently used histological dyes chromaticity coordinates, has been carried out.

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Literatur
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Zurück zum Zitat T. A. Kravtsova, D. A. Dobrolyubova, and A. V. Samorodov, “Comparatively studied color correction methods for color calibration of automated microscopy complex of biomedical specimens,” Nauka i Obraz. MGTU im. Baumana, No. 2, 91–104 (2016), http://engineering-science.ru/doc/833329.html, acc. Sept. 1, 2021. T. A. Kravtsova, D. A. Dobrolyubova, and A. V. Samorodov, “Comparatively studied color correction methods for color calibration of automated microscopy complex of biomedical specimens,” Nauka i Obraz. MGTU im. Baumana, No. 2, 91–104 (2016), http://​engineering-science.​ru/​doc/​833329.​html, acc. Sept. 1, 2021.
Metadaten
Titel
Color Calibration Slide for Digital Microscopy Systems
verfasst von
D. S. Makhov
G. R. Sagatelyan
A. V. Samorodov
Publikationsdatum
12.03.2022
Verlag
Springer US
Erschienen in
Measurement Techniques / Ausgabe 10/2022
Print ISSN: 0543-1972
Elektronische ISSN: 1573-8906
DOI
https://doi.org/10.1007/s11018-022-02014-7

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