Cytogenetic Effects of Humic Substances and Their Use for Remediation of Polluted Environments

Mechanisms of humic substances (HS) physiological activity in relation to different organisms at a molecular—cellular level were studied. Adaptation mechanisms of HS action under radiation and chemical injuries were examined. Antimutagenic and detoxifying properties of HS were revealed and a usage of physiologically active HS for remediation of both biotic and abiotic components of the environments and human's health recovery was proved. By the example of industrial area nearby the Dnepr River the HS applicability for remediation of disturbed soil was demonstrated. Experimental evidences of reduction of genetic damage caused by chemical and physical stress factors in the presence of HS were obtained using various bioassay techniques. A theoretical basis for HS application to retard uptake of contaminants by plants from soil and to improve biota resistance towards anthropogenic factors was developed. Recommendations on improving environmental conditions, recovering human health, and remediation of polluted areas were elaborated based on application of HS.