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Erschienen in:

2015 | Online First | Buchkapitel

Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells

verfasst von : Işıl Karanfil, Tugba Bagci-Onder

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Neural stem cells (NSCs) derived from induced pluripotent stem cells offer therapeutic tools for neurodegenerative diseases. This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs). Generation of healthy and stable NSCs from iPSCs heavily depends on standardized in vitro cell culture systems that mimic the embryonic environments utilized during neural development. Specifically, the neural induction and expansion methods after EB formation are described in this review.

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Titel: Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells
verfasst von: Işıl Karanfil
Tugba Bagci-Onder
Verlag: Springer New York
DOI: https://doi.org/10.1007/7651_2015_227

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Abstract

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