2006 | OriginalPaper | Buchkapitel
Field Portable and Autonomous Immunosensors for the Detection of Environmental Contaminants
verfasst von : Diane A. Blake, Haini Yu, Elizabeth A. James, Xia Li, Robert C. Blake
Erschienen in: Uranium in the Environment
Verlag: Springer Berlin Heidelberg
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A monoclonal antibody that binds with high affinity to UO22+ complexed with the chelator 2,9-dicarboxyl-1,10-phenanthroline was used to develop a sensor-based assay for UO22+. The assay range for UO22+ was 0.5 to 25 nM (0.12 to 6 ppb). The average coefficients of variation in the assay was 2.2%. The immunoassay results were comparable to those obtained using the Kinetic Phosphorescence Assay. The versatility of this sensor platform was further demonstrated by the development of an immunoassay for caffeine.