Skip to main content
Fachgebiete
Automobil + Motoren Bauwesen + Immobilien Business IT + Informatik Elektrotechnik + Elektronik Energie + Nachhaltigkeit Finance + Banking Management + Führung Marketing + Vertrieb Maschinenbau + Werkstoffe Versicherung + Risiko
DE
EN
Bücher
Zeitschriften
Themenseiten
Organisationspsychologie Projektmanagement Marketing Smart Manufacturing
Weitere Formate
Podcasts Webinare Technik Webinare Wirtschaft Kongresse Veranstaltungskalender Awards
Jetzt Einzelzugang starten
Zugang für Unternehmen
Referenzkunden
SLX-Digitalkonferenz: Zukunftswerkstatt 2024

Mehr Umsatz mit Top-Kontakten

Am 7. Mai erfahren Sie, wie Vertriebsteams Leadgenerierung, Leadnurturing und Leadstrategien effizient steuern können.
Erweiterte Suche
Anmelden
nach oben

2011 | Buch

What Is This Book About? Kapitel lesen Erstes Kapitel lesen

Bioreaction Engineering Principles

verfasst von: John Villadsen, Jens Nielsen, Gunnar Lidén

Verlag: Springer US

Enthalten in: Springer Professional "Wirtschaft+Technik" , Springer Professional "Technik"

Einloggen, um Zugang zu erhalten
insite
SUCHEN

Über dieses Buch

The present text is a complete revision of the 2nd edition from 2003 of the book with the same title. In recognition of the fast pace at which biotechnology is moving we have rewritten several chapters to include new scientific progress in the field from 2000 to 2010. More important we have changed the focus of the book to support its use, not only in universities, but also as a guide to design new processes and equipment in the bio-industry. A new chapter has been included on the prospects of the bio-refinery to replace many of the oil- and gas based processes for production of especially bulk chemicals. This chapter also serves to make students in Chemical Engineering and in the Bio-Sciences enthusiastic about the whole research field. As in previous editions we hope that the book can be used as textbook for classes, even at the undergraduate level, where chemical engineering students come to work side by side with students from biochemistry and microbiology. To help the chemical engineering students Chapter 1 includes a brief review of the most important parts of microbial metabolism. In our opinion this review is sufficient to understand microbial physiology at a sufficiently high level to profit from the rest of the book. Likewise the bio-students will not be overwhelmed by mathematics, but since the objective of the book is to teach quantitative process analysis and process design at a hands-on level some mathematics and model analysis is needed. We hope that the about 100 detailed examples and text notes, together with many instructive problems will be sufficient to illustrate how model analysis is used, also in Bio-reaction Engineering.

Inhaltsverzeichnis

Frontmatter
Chapter 1. What Is This Book About?
Abstract
Looking back to the introductory Chap. 1 of the first and second editions of Bioreaction Engineering Principles, we still find that the schematic representation of topics in Fig. 1.1 adequately describes what we believe should be the focus of the third edition.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 2. Chemicals from Metabolic Pathways
Abstract
For the past 80–90 years petroleum and natural gas have served as raw materials for the majority of the finished products of our daily lives. After World War II these raw materials decisively substituted coal, and they have been the foundation of an enormous increase in material wealth and welfare throughout the World.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 3. Elemental and Redox Balances
Abstract
In Chap. 2 we painted a picture of the potential of Biotechnology as the provider of a great many of the chemicals used in our daily life. Now, in small steps the quantitative tools for analysis of the cellular reactions will be introduced. First, the rates of cellular reactions will be determined by application of mass balances to data obtained in steady-state continuous bioreactors.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 4. Thermodynamics of Bioreactions
Abstract
The Black Box models of Chap. 3, based as they are on mass balances in continuous steady-state reactors, are the pale reflections of the long biochemical pathways of Chap. 2.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 5. Biochemical Reaction Networks
Abstract
Section 2.2 introduced the pathways of cells, and in Chap. 3 the steady-state conversion of substrates to final metabolic products was described using a single stoichiometric equation, the black box model. The stoichiometric coefficients, or rather the yield coefficients, because they vary with the cultivation conditions, were determined using mass and redox balances. In Chap. 4, the black box model was examined using the tools of thermodynamics to determine if a given overall reaction was feasible from a thermodynamic perspective. In this chapter, the network of pathways through which substrates are converted to products are studied more closely. Specifically, the distribution of carbon from the substrate(s) to the different products is calculated.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 6. Enzyme Kinetics and Metabolic Control Analysis
Abstract
In all probability, the reader of this text has encountered the action of individual enzymes long before becoming aware of the concerted action of the many enzymes that together determine the flow of substrates through metabolic pathways of living cells. It could have been in high school experiments where catalase was used to liberate O2 from H2O2, or by studying how “the enzyme pellets” from a box of a commercial detergent product “dissolved” starch grains.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 7. Growth Kinetics of Cell Cultures
Abstract
In Chaps. 3 and 5, we have discussed how the two important design parameters for cell cultures, yield and productivity, can be derived from experimental data, e.g., from measurements of the substrate consumption and the product formation. Furthermore, we have shown how measured steady-state fluxes in and out of the cell, the exchange fluxes, can be used to calculate the fluxes through the different branches of the metabolic network of a given microorganism.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 8. Population Balance Equations
Abstract
In Chap. 7, cell population balances are written in terms of a distribution of mass fractions of the total biomass. This allows a direct combination of intracellularly structured models and population models. However, the population balances based on mass fractions do not permit the incorporation into the model of specific events in the cell cycle. Since there are numerous examples that show a direct influence of certain specific events in the cell cycle on the overall culture performance, e.g., the distribution of plasmids to daughter cells on cell division in recombinant cultures, we need to derive a population balance based on cell number to obtain a correct description of these processes.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 9. Design of Fermentation Processes
Abstract
In previous chapters, the stoichiometry of bioreactions has been investigated, and it was shown that how steady-state rates could be measured for many reaction components. In Chaps. 3 and 4, the extent of the bioreaction was determined as a function of the reaction conditions, in particular, the feed composition, the redox level, and pH. In Chap. 5, the black box model for a bioreaction was greatly expanded to include a sub-set of the almost infinite number of reactions in the metabolic network. Finally, in Chaps. 6 and 7, the chemical reaction, first in enzyme reactions and then in cell cultivations, was treated by the same tools as are used in “ordinary” chemical reactions: The reaction rate was described as a function of the concentrations of reactants and afterwards included in mass balances.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 10. Gas–Liquid Mass Transfer
Abstract
The first requirement for any chemical reaction to take place is that the reactants are present at the site of reaction. In multiphase systems, rates of transport processes are often smaller than the intrinsic maximum reaction rates. The result is that the actual reaction rate is smaller than what would be anticipated from kinetics alone. For the unprepared mind this may come as both a surprise and a disappointment. Transport phenomena played a role for diffusion in immobilized enzyme systems in Sect. 6.3.2, where the basic mathematical models for analysis of combined mass transfer and kinetics were introduced. In biological multiphase systems, mass transfer takes place between two liquid phases, between a liquid and a solid phase, or between a gas and a liquid phase.
John Villadsen, Jens Nielsen, Gunnar Lidén
Chapter 11. Scale-Up of Bioprocesses
Abstract
The previous chapters in this book have dealt with stoichiometric, thermodynamic and kinetic analysis of bioreactions, as well as the operation of small-scale, “ideal,” bioreactors. These subjects constitute the basis for exploitation of microorganisms in fermentation processes. The ultimate goal for process development is, however, the realization of large-scale commercial production. The basis for a successful scale-up is to follow the advice of H. Baekeland, the inventor of Bakelite: “Commit your blunders on a small scale and make your profits on a large scale.” This is, however, not always easy. Many different engineering tools need to be applied (Leib et al. 2001), and the final scaled-up process will necessarily be a delicate compromise between inherently conflicting desirable options. Furthermore, even if the best engineering judgment is used, there are sometimes surprises in the final process which were difficult to anticipate from the lab-scale experiments.
John Villadsen, Jens Nielsen, Gunnar Lidén
Backmatter
Metadaten
Titel
Bioreaction Engineering Principles
verfasst von
John Villadsen
Jens Nielsen
Gunnar Lidén
Copyright-Jahr
2011
Verlag
Springer US
Electronic ISBN
978-1-4419-9688-6
Print ISBN
978-1-4419-9687-9
DOI
https://doi.org/10.1007/978-1-4419-9688-6

Premium Partner

    Marktübersichten

    Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen. 

    Zur Marktübersicht
    Bildnachweise