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Experiments in Fluids
Erschienen in: Experiments in Fluids 5/2008

01.05.2008 | Research Article

Droplet streams for serial crystallography of proteins

verfasst von: U. Weierstall, R. B. Doak, J. C. H. Spence, D. Starodub, D. Shapiro, P. Kennedy, J. Warner, G. G. Hembree, P. Fromme, H. N. Chapman

Erschienen in: Experiments in Fluids | Ausgabe 5/2008

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Abstract

Serial diffraction of proteins requires an injection method to deliver analyte molecules—preferably uncharged, fully hydrated, spatially oriented, and with high flux—into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.
Nächster Artikel Scale effects in subaerial landslide generated impulse waves

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Metadaten
Titel
Droplet streams for serial crystallography of proteins
verfasst von
U. Weierstall
R. B. Doak
J. C. H. Spence
D. Starodub
D. Shapiro
P. Kennedy
J. Warner
G. G. Hembree
P. Fromme
H. N. Chapman
Publikationsdatum
01.05.2008
Verlag
Springer-Verlag
Erschienen in
Experiments in Fluids / Ausgabe 5/2008
Print ISSN: 0723-4864
Elektronische ISSN: 1432-1114
DOI
https://doi.org/10.1007/s00348-007-0426-8

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