nach oben

Journal of Nanoparticle Research

Erschienen in:

01.09.2011 | Research Paper

Biological impacts of TiO₂ on human lung cell lines A549 and H1299: particle size distribution effects

verfasst von: Roslyn Tedja, Christopher Marquis, May Lim, Rose Amal

Erschienen in: Journal of Nanoparticle Research | Ausgabe 9/2011

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

Increasing use of titanium dioxide (TiO₂) nanoparticles in many commercial applications has led to emerging concerns regarding the safety and environmental impact of these materials. In this study, we have investigated the biological impact of nano-TiO₂ (with particle primary size of 20 nm Aeroxide P25) on human lung cell lines in vitro and also the effect of particle size distribution on the particle uptake and apparent toxicity. The biological impact of nano-TiO₂ is shown to be influenced by the concentration and particle size distribution of the TiO₂ and the impact was shown to differ between the two cell lines (A549 and H1299) investigated herein. A549 cell line was shown to be relatively resistant to the total amount of TiO₂ particles uptaken, as measured by cell viability and metabolic assays, while H1299 had a much higher capacity to ingest TiO₂ particles and aggregates, with consequent evidence of impact at concentrations as low as 30–150 μg/mL TiO₂. Evidence gathered from this study suggests that both viability and metabolic assays (measuring metabolic and mitochondrial activities and also cellular ATP level) should be carried out collectively to gain a true assessment of the impact of exposure to TiO₂ particles.

Vorheriger Artikel Characterization and applications of as-grown β-Fe2O3 nanoparticles prepared by hydrothermal method

Nächster Artikel Infra-red assisted sintering of inkjet printed silver tracks on paper substrates

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Nur mit Berechtigung zugänglich

Adams LK, Lyon DY, Alvarez PJJ (2006) Comparative eco-toxicity of nanoscale TiO₂, SiO₂, and ZnO water suspensions. Water Res 40(19):3527–3532CrossRef

AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3(2):279–290CrossRef

Baggs RB, Ferin J, Oberdorster G (1997) Regression of pulmonary lesions produced by inhaled titanium dioxide in rats. Vet Pathol 34(6):592–597CrossRef

Baraton MI, Merhari L (2004) Surface chemistry of TiO₂ nanoparticles: influence on electrical and gas sensing properties. J Eur Ceram Soc 24(6):1399–1404CrossRef

Barillet S, Simon-Deckers A, Herlin-Boime N, Mayne-L’Hermite M, Reynaud C, Cassio D, Gouget B, Carrière M (2010) Toxicological consequences of TiO₂, SiC nanoparticles and multi-walled carbon nanotubes exposure in several mammalian cell types: an in vitro study. J Nanopart Res 12(1):61–73CrossRef

Beydoun D, Amal R, Low G, McEvoy S (1999) Role of nanoparticles in photocatalysis. J Nanopart Res 1(4):439–458CrossRef

Bickley RI, Gonzalez-Carreno T, Lees JS, Palmisano L, Tilley RJD (1991) A structural investigation of titanium dioxide photocatalysts. J Solid State Chem 92(1):178–190CrossRef

Cormier SA, Lomnicki S, Backes W, Dellinger B (2006) Origin and health impacts of emissions of toxic by-products and fine particles from combustion and thermal treatment of hazardous wastes and materials. Environ Health Perspect 114(6):810–817CrossRef

Ferin J, Oberdörster G, Penney DP (1992) Pulmonary retention of fine and ultrafine particles in rats. Am J Respir Cell Mol Biol 6:535–542

Friedlander S, Pui D (2004) Emerging issues in nanoparticle aerosol science and technology. J Nanopart Res 6(2):313–320CrossRef

Gao H, Shi W, Freund LB (2005) Mechanics of receptor-mediated endocytosis. Proc Natl Acad Sci 102(27):9469–9474CrossRef

Grassian VH, Adamcakova-Dodd A, Pettibone JM, O’Shaughnessy PT, Thorne PS (2007) Inflammatory response of mice to manufactured titanium dioxide nanoparticles: comparison of size effects through different exposure routes. Nanotoxicology 1(3):211–226CrossRef

Gunawan C, Teoh WY, Marquis CP, Juniahani Lifia, Amal R (2009) Reversible antimicrobial photoswitching in nanosilver. Small 5(3):341–344CrossRef

Gurr JR, Wang ASS, Chen CH, Jan KY (2005) Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 213(1–2):66–73CrossRef

Horie M, Nishio K, Fujita K, Endoh S, Miyauchi A, Saito Y, Iwahashi H, Yamamoto K, Murayama H, Nakano H, Nanashima N, Niki E, Yoshida Y (2009) Protein adsorption of ultrafine metal oxide and its influence on cytotoxicity toward cultured cells. Chem Res Toxicol 22(3):543–553CrossRef

Inoue A, Narumi K, Matsubara N, Sugawara S-i, Saijo Y, Satoh K, Nukiwa T (2000) Administration of wild-type p53 adenoviral vector synergistically enhances the cytotoxicity of anti-cancer drugs in human lung cancer cells irrespective of the status of p53 gene. Cancer Lett 157(1):105–112CrossRef

Ito I, Began G, Mohiuddin I, Saeki T, Saito Y, Branch CD, Vaporciyan A, Clifton Stephens L, Yen N, Roth JA, Ramesh R (2003) Increased uptake of liposomal–DNA complexes by lung metastases following intravenous administration. Mol Ther 7(3):409–418CrossRef

Jiang J, Oberdörster G, Biswas P (2009) Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. J Nanopart Res 11(1):77–89CrossRef

Kang SJ, Kim BM, Lee YJ, Chung HW (2008) Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes. Environ Mol Mutagen 49(5):399–405CrossRef

Kho YK, Iwase A, Teoh WY, MaÌˆdler L, Kudo A, Amal R (2010) Photocatalytic H₂ evolution over TiO₂ nanoparticles. The synergistic effect of anatase and rutile. J Phys Chem C 114(6):2821–2829CrossRef

Lam SW, Soetanto A, Amal R (2009) Self-cleaning performance of polycarbonate surfaces coated with titania nanoparticles. J Nanopart Res 11(8):1971–1979CrossRef

Lee Y, Yoon S, Park M, Kim J, Lee J, Song C (2010) Influence of p53 expression on sensitivity of cancer cells to bleomycin. J Biochem Mol Toxicol 24:260–269. doi:10.1002/jbt.20334 CrossRef

Li J, Li Q, Xu J, Li J, Cai X, Liu R, Li Y, Ma J, Li W (2007) Comparative study on the acute pulmonary toxicity induced by 3 and 20 nm TiO₂ primary particles in mice. Environ Toxicol Pharmacol 24(3):239–244CrossRef

Lieber M, Todaro G, Smith B, Szakal A, Nelson-Rees W (1976) A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer 17(1):62–70CrossRef

Liu S, Lim M, Fabris R, Chow C, Drikas M, Amal R (2008) TiO₂ photocatalysis of natural organic matter in surface water: impact on trihalomethane and haloacetic acid formation potential. Environ Sci Technol 42(16):6218–6223. doi:10.1021/es800887s CrossRef

Liu S, Xu L, Zhang T, Ren G, Yang Z (2010) Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells. Toxicology 267(1–3):172–177CrossRef

Mo Y, Lim L-Y (2005) Preparation and in vitro anticancer activity of wheat germ agglutinin (WGA)-conjugated PLGA nanoparticles loaded with paclitaxel and isopropyl myristate. J Control Release 107(1):30–42CrossRef

Murdock RC, Braydich-Stolle L, Schrand AM, Schlager JJ, Hussain SM (2008) Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicol Sci 101(2):239–253CrossRef

Nohynek GJ, Lademann J, Ribaud C, Roberts MS (2007) Grey goo on the skin? Nanotechnology, cosmetic and sunscreen safety. Crit Rev Toxicol 37(3):251–277CrossRef

Oberdörster G (2000) Pulmonary effects of inhaled ultrafine particles. Int Arch Occup Environ Health 74(1):1–8CrossRef

Ohno T, Sarukawa K, Tokieda K, Matsumura M (2001) Morphology of a TiO₂ photocatalyst (Degussa, P25) consisting of anatase and rutile cyrstalline phases. J Catal 203:82–86CrossRef

Oren M (2003) Decision making by p53: life, death and cancer. Cell Death Differ 10(4):431–442CrossRef

Powers KW, Brown SC, Krishna VB, Wasdo SC, Moudgil BM, Roberts SM (2006) Research strategies for safety evaluation of nanomaterials. Part VI. Characterization of nanoscale particles for toxicological evaluation. Toxicol Sci 90(2):296–303CrossRef

Qian L, Hinestroza JP (2004) Application of nanotechnology for high performance textiles. J Text Appar Technol Manag 4(1):1–7

Sayes CM, Wahi R, Kurian PA, Liu Y, West JL, Ausman KD, Warheit DB, Colvin VL (2006) Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol Sci 92(1):174–185CrossRef

Shimamura A, Fisher DE (1996) p53 in life and death. Clin Cancer Res 2(3):435

Silverstein SC, Steinman RM, Cohn ZA (1977) Endocytosis. Annu Rev Biochem 46(1):669–722CrossRef

Stearns RC, Paulauskis JD, Godleski JJ (2001) Endocytosis of ultrafine particles by A549 cells. Am J Respir Cell Mol Biol 24(2):108–115

Thevenot P, Cho J, Wavhal D, Timmons RB, Tang L (2008) Surface chemistry influences cancer killing effect of TiO₂ nanoparticles. Nanomed: Nanotechnol Biol Med 4(3):226–236CrossRef

Van Hoecke K, Quik JT, Mankiewicz-Boczek J, De Schamphelaere KA, Elsaesser A, Van der Meeren P, Barnes C, McKerr G, Howard CV, Van de Meent D (2009) Fate and effects of CeO₂ nanoparticles in aquatic ecotoxicity tests. Environ Sci Technol 43(12):4537CrossRef

Vaux D (1993) Toward an understanding of the molecular mechanisms of physiological cell death. Proc Natl Acad Sci 90(3):786–789CrossRef

Wang J, Zhou G, Chen C, Yu H, Wang T, Ma Y, Jia G, Gao Y, Li B, Sun J, Li Y, Jiao F, Zhao Y, Chai Z (2007) Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. Toxicol Lett 168(2):176–185CrossRef

Warheit DB, Webb TR, Sayes CM, Colvin VL, Reed KL (2006) Pulmonary instillation studies with nanoscale TiO₂ rods and dots in rats: toxicity is not dependent upon particle size and surface area. Toxicol Sci 91(1):227CrossRef

Warheit DB, Webb TR, Reed KL, Frerichs S, Sayes CM (2007) Pulmonary toxicity study in rats with three forms of ultrafine-TiO₂ particles: differential responses related to surface properties. Toxicology 230(1):90–104CrossRef

Yin Win K, Feng SS (2005) Effects of particle size and surface coating on cellular uptake of polymeric nanoparticles for oral delivery of anticancer drugs. Biomaterials 26(15):2713–2722CrossRef

Young C, Lim TM, Chiang K, Scott J, Amal R (2008) Photocatalytic oxidation of toluene and trichloroethylene in the gas-phase by metallised (Pt, Ag) titanium dioxide. Appl Catal B Environ 78(1–2):1–10CrossRef

Titel: Biological impacts of TiO2 on human lung cell lines A549 and H1299: particle size distribution effects
verfasst von: Roslyn Tedja
Christopher Marquis
May Lim
Rose Amal
Publikationsdatum: 01.09.2011
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 9/2011
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-011-0302-6

Premium Partner

Marktübersichten

Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.

Zur Marktübersicht

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Weitere Artikel der Ausgabe 9/2011

Enhancement of third-order nonlinearity in Ag-nanoparticles-contained chalcohalide glasses

Effect of silver nanoparticles with different shapes on luminescence of samarium complex at two different excitation wavelengths

Mesoscopic phenomena in oxide nanoparticles systems: processes of growth

GoldMag nanoparticles with core/shell structure: characterization and application in MR molecular imaging

Synthesis and characterization of carbon coated nanoparticles produced by a continuous low-pressure plasma process

On the role of the colloidal stability of mesoporous silica nanoparticles as gene delivery vectors

Premium Partner

Marktübersichten