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Journal of Nanoparticle Research

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01.07.2015 | Research Paper

Preparation of thermo and pH-responsive polymer@Au/Fe₃O₄ core/shell nanoparticles as a carrier for delivery of anticancer agent

verfasst von: Marjan Ghorbani, Hamed Hamishehkar, Naser Arsalani, Ali Akbar Entezami

Erschienen in: Journal of Nanoparticle Research | Ausgabe 7/2015

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Abstract

In this work, a thermo and pH-responsive poly-N-isopropylacrylamide-co-itaconic acid containing thiol side groups were successfully synthesized to prepare Doxorubicin-loaded polymer@Au/Fe₃O₄ core/shell nanoparticles (DOX-NPs). Copolymer and NPs were fully characterized by FT-IR, HNMR, photo-correlation spectroscopy, SEM, X-ray diffraction, vibrating-sample magnetometer, thermal gravimetric analysis, and UV–Vis spectroscopy. The stimuli-responsive characteristics of NPs were evaluated by in vitro release study in simulated cancerous environment. The biocompatibility and cytotoxic properties of NPs and DOX-NPs are explored by MTT method. The prepared NPs with the size of 50 nm showed paramagnetic characteristics with suitable and stable dispersion at physiological medium and high loading capacity (up to 55 %) of DOX. DOX-NPs yielded a pH- and temperature-triggered release of entrapped drugs at tumor tissue environment (59 % of DOX release) compared to physiological condition (20 % of DOX release) during 48 h. In vitro cytotoxicity studies indicated that the NPs showed no cytotoxicity on A549 cells at different amounts after incubation for 72 h confirming its suitability as a drug carrier. DOX-NPs, on the other hand, caused an efficient anticancer performance as verified by MTT assay test. It was concluded that developed NPs by us in this study may open the possibilities for targeted delivery of DOX to the cancerous tissues.

Vorheriger Artikel Characterization and electrocatalytic activity of Pt–M (M=Cu, Ag, and Pd) bimetallic nanoparticles synthesized by pulsed plasma discharge in water

Nächster Artikel Heterocyclic aramid nanoparticle-assisted graphene exfoliation for fabrication of pristine graphene-based composite paper

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Bawa P, Pillay V, Choonara YE, Toit LC (2009) Stimuli-responsive polymers and their applications in drug delivery. Biomed Mater 4:1–15. doi:10.1088/1748-6041/4/2/022001 CrossRef

Chao X, Shi F, Zhao YY, Li K, Peng M, Chen C, Cui YL (2010) Cytotoxicity of Fe₃O₄/Au composite nanoparticles loaded with doxorubicin combined with magnetic field. Pharmazie 65:500–504. doi:10.1691/ph.2010.9362

Chao X, Guo L, Zhao Y, Hua K, Peng M, Chen C, Cui Y (2011) PEG-modified GoldMag nanoparticles (PGMNs) combined with the magnetic field for local drug delivery. J Drug Target 19:161–170. doi:10.3109/10611861003801842 CrossRef

Cho K, Wang X, Nie S, Chen Z, Shin DM (2008) Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 14:1310–1316. doi:10.1158/1078-0432.CCR-07-1441 CrossRef

Cui Y, Hui W, Su J, Wang Y, Chen C (2005a) Fe₃O₄/Au composite nano-particles and their optical properties. Sci China, Ser B 48:273–278. doi:10.1360/042004-51 CrossRef

Cui Y, Wang Y, Hui W, Zhang Z, Xin X, Chen C (2005b) The synthesis of GoldMag nano-particles and their application for antibody immobilization. Biomed Microdevices 7:153–156. doi:10.1007/s10544-005-1596-x CrossRef

Cui W, Lu X, Cui K, Niu L, Wei Y, Lu Q (2012) Dual-responsive controlled drug delivery based on ionically assembled nanoparticles. Langmuir 28:9413–9420. doi:10.1021/la3016436 CrossRef

Du B, Mei A, Tao P, Zhao B, Cao Z, Nie J, Xu J, Fan Z (2009) Poly[N -isopropylacrylamide- co -3-(trimethoxysilyl)-propylmethacrylate] coated aqueous dispersed thermosensitive Fe₃O₄ nanoparticles. J Phys Chem C 113:10090–10096. doi:10.1021/jp9016536 CrossRef

Dykman L, Khlebtsov N (2012) Gold nanoparticles in biomedical applications: recent advances and perspectives. Chem Soc Rev 41:2256–2282. doi:10.1039/c1cs15166e CrossRef

Ebright YW, Chen Y, Pendergrast PS, Ebright RH (1992) Incorporation of an EDTA-metal complex at a rationally selected site within a protein: application to EDTA-iron DNA affinity cleaving with catabolite gene activator protein (CAP) and Cro. Biochemistry 31:10664–10670. doi:10.1021/bi00159a004 CrossRef

Ganta S, Devalapally H, Shahiwala A, Amiji M (2008) A review of stimuli-responsive nanocarriers for drug and gene delivery. J Control Release 126:187–204. doi:10.1016/j.jconrel.2007.12.017 CrossRef

Hamner KL, Maye MM (2013) Thermal aggregation properties of nanoparticles modified with temperature sensitive copolymers. Langmuir 29:15217–15223. doi:10.1021/la4037887 CrossRef

Hamner KL, Alexander CM, Coopersmith K, Reishofer D, Provenza C, Maye MM (2013) Using temperature-sensitive smart polymers to regulate DNA-mediated nanoassembly and encoded nanocarrier drug release. ACS Nano 7:7011–7020. doi:10.1021/nn402214e CrossRef

Kast CE, Bernkop-Schnürch A (2002) Polymer–cysteamine conjugates: new mucoadhesive excipients for drug delivery? Int J Pharm 234:91–99. doi:10.1016/S0378-5173(01)00955-3 CrossRef

Lee H, Fonge H, Hoang B, Reilly RM, Allen C (2010) The effects of particle size and molecular targeting on the intratumoral and subcellular distribution of polymeric nanoparticles. Mol Pharm 7:1195–1208. doi:10.1021/mp100038h CrossRef

Li C, Chen T, Ocsoy I, Zhuet C, Yasun E, You M, Wu C, Zheng J, Song E, Huang CZ, Tan W (2014a) Gold-Coated Fe₃O₄ nanoroses with five unique functions for cancer cell targeting, imaging and therapy. Adv Funct Mater 24:1772–1780. doi:10.1002/adfm.201301659 CrossRef

Li J, Wu W, Han C, Zhang S, Zhou H, Guo J (2014b) Aggregation behavior of pH- and thermo-responsive block copolymer protected gold nanoparticles. Colloid Polym Sci 292:1657–1664. doi:10.1007/s00396-014-3225-9 CrossRef

Liang Z, Wu X, Xie Y, Liu S (2012) A facile Approach to fabricate water-soluble Au–Fe₃O₄ nanoparticle for liver cancer cells imaging. Chin J Chem 30:1387–1392. doi:10.1002/cjoc.201100692 CrossRef

Lin JJ, Chen JS, Huang SJ, Ko JH, Wang YM, Chen TL, LiF Wang (2009) Folic acid-Pluronic F127 magnetic nanoparticle clusters for combined targeting, diagnosis, and therapy applications. Biomaterials 30:5114–5124. doi:10.1016/j.biomaterials.2009.06.004 CrossRef

Liu TY, Hu SH, Liu TY, Liu DM, Chen SY (2006) Magnetic-sensitive behavior of intelligent ferrogels for controlled release of drug. Langmuir 22:5974–5978. doi:10.1021/la060371e CrossRef

Liu H, Hou P, Zhang W, Kim YK, Wu JH (2010) The synthesis and characterization of polymer-coated FeAu multifunctional nanoparticles. Nanotechnology 21:1–9. doi:10.1088/0957-4484/21/33/335602

Liu J, Huang Y, Kumar A, Tan A, Jin S, Mozhi A, Liang X-J et al (2014) pH-sensitive nano-systems for drug delivery in cancer therapy. Biotechnol Adv 32:693–710. doi:10.1016/j.biotechadv.2013.11.009 CrossRef

Lo CK, Xiao D, Choi MMF (2007) Homocysteine-protected gold-coated magnetic nanoparticles: synthesis and characterisation. J Mater Chem 17:2418–2427. doi:10.1039/b617500g CrossRef

Lowe AB, Sumerlin BS, Donovan MS, McCormick CL (2002) Facile preparation of transition metal nanoparticles stabilized by well-defined (co)polymers synthesized via aqueous reversible addition-fragmentation chain transfer polymerization. J Am Chem Soc 124:11562–11563. doi:10.1021/ja020556h CrossRef

Mahmoudi M, Sant S, Wang B, Laurent S, Senf T (2011) Superparamagnetic iron oxide nanoparticles (SPIONs): development, surface modification and applications in chemotherapy. Adv Drug Deliv Rev 63:24–46. doi:10.1016/j.addr.2010.05.006 CrossRef

Massart R (1981) Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE Trans Magn 17:1247–1248. doi:10.1109/TMAG.1981.1061188 CrossRef

Mohanraj VJ, Chen Y (2006) Nanoparticles—A review. Trop J Pharm Res 5:561–573. doi:10.4314/tjpr.v5i1.14634

Nigam S, Chandra S, Newgreen DF, Bahadur D, Chen Q (2014) Poly(ethylene glycol)-modified PAMAM-Fe₃O₄-doxorubicin triads with the potential for improved therapeutic efficacy: generation-dependent increased drug loading and retention at neutral pH and increased release at acidic pH. Langmuir 30:1004–1011. doi:10.1021/la404246h CrossRef

Nuopponen M, Tenhu H (2007) Gold nanoparticles protected with pH and temperature-sensitive diblock copolymers. Langmuir 23:5352–5357. doi:10.1021/la063240m CrossRef

Ossipov D, Piskounova S, Hilborn J (2008) Poly (vinyl alcohol) cross-linkers for in vivo injectable hydrogels. Macromolecules 41:3971–3982. doi:10.1021/ma800332c CrossRef

Sahoo B, Devi KSP, Banerjee R, Maiti TK, Pramanik P, Dhara D (2013) Thermal and pH responsive polymer-tethered multifunctional magnetic nanoparticles for targeted delivery of anticancer drug. ACS Appl Mater Interfaces 5:3884–3893. doi:10.1021/am400572b CrossRef

Salehi R, Hamishehkar H (2014) Development of dual responsive nanocomposite for simultaneous delivery of anticancer drugs. J Drug Target 22:327–342. doi:10.3109/1061186X.2013.876645 CrossRef

Salehizadeh H, Hekmatian E, Sadeghi M, Kennedy K (2012) Synthesis and characterization of core-shell Fe₃O₄-gold-chitosan nanostructure. J Nanobiotechnol 10:1–7. doi:10.1186/1477-3155-10-3 CrossRef

Salmaso S, Caliceti P, Amendola V, Meneghetti M, Magnusson JP, Pasparakis G, Alexander C (2009) Cell up-take control of gold nanoparticles functionalized with a thermoresponsive polymer. J Mater Chem 19:1608–1615. doi:10.1039/b816603j CrossRef

Shi F, Hui W, Cui Y, Chen C (2011) Surface modification and characterization of Fe₃O₄/Au composite nanoparticles. Nano 6:145–151. doi:10.1142/S1793292011002469 CrossRef

Shi S, Wang Q, Wang T, Ren S, Gao Y, Wang N (2014) Thermo-, pH-, and light-responsive poly(N-isopropylacrylamide-co-methacrylic acid)–Au hybrid microgels prepared by the in situ reduction method based on Au-thiol chemistry. J Phys Chem B 118:7177–7186. doi:10.1021/jp5027477 CrossRef

Siegel RA (2014) Stimuli sensitive polymers and self regulated drug delivery systems: a very partial review. J Control Release 190:337–351. doi:10.1016/j.jconrel.2014.06.035 CrossRef

Sundaresan V, Menon JU, Rahimi M, Nguyen KT, Wadajkar AS (2014) Dual-responsive polymer-coated iron oxide nanoparticles for drug delivery and imaging applications. Int J Pharm 466:1–7. doi:10.1016/j.ijpharm.2014.03.016 CrossRef

Tamer U, Gündoğdu Y, Boyacı İH, Pekmez K (2009) Synthesis of magnetic core–shell Fe₃O₄–Au nanoparticle for biomolecule immobilization and detection. J Nanoparticle Res 12:1187–1196. doi:10.1007/s11051-009-9749-0 CrossRef

Yang HM, Park CW, Bae PK, Ahn T, Seo BK, Chung BH, Kim J-D (2013) Folate-conjugated cross-linked magnetic nanoparticles as potential magnetic resonance probes for in vivo cancer imaging. J Mater Chem B 1:3035–3043. doi:10.1039/c3tb20295j CrossRef

Yusa S, Fukuda K, Yamamoto T, Iwasaki Y, Watanabe A, Akiyoshi K, Morishima Y (2007) Salt effect on the heat-induced association behavior of gold nanoparticles coated with poly(N-isopropylacrylamide) prepared via reversible addition-fragmentation chain transfer (RAFT) radical polymerization. Langmuir 23:12842–12848. doi:10.1021/la702741q CrossRef

Zhang Z, Maji S, da Antunes ABF, Rycke RD, Zhang Q, Hoogenboom R, de Geest BG (2013) Salt plays a pivotal role in the temperature-responsive aggregation and layer-by-layer assembly of polymer-decorated gold nanoparticles. Chem Mater 25:4297–4303. doi:10.1021/cm402414u CrossRef

Zhang W, Deng L, Wang G, Guo X, Li Q, Zhang J, Khashab NM (2014) Low-magnetization magnetic microcapsules: a synergistic theranostic platform for remote cancer cells therapy and imaging. Part Part Syst Charact 31:985–993. doi:10.1002/ppsc.201400005 CrossRef

Zhao X, Cai Y, Wang T, Shi Y, Jiang G (2008) Preparation of alkanethiolate-functionalized core/shell Fe₃O₄@Au nanoparticles and its interaction with several typical target molecules. Anal Chem 80:9091–9096. doi:10.1021/ac801581m CrossRef

Zhao Z, Huang D, Yin Z, Chi X, Wang X, Gao J (2012) Magnetite nanoparticles as smart carriers to manipulate the cytotoxicity of anticancer drugs: magnetic control and pH-responsive release. J Mater Chem 22:15717–15725. doi:10.1039/c2jm31692g CrossRef

Zheng P, Jiang X, Zhang X, Zhang W, Shi L (2006) Formation of gold@polymer core-shell particles and gold particle clusters on a template of thermoresponsive and pH-responsive coordination triblock copolymer. Langmuir 22:9393–9396. doi:10.1021/la0609064 CrossRef

Zhu Y-J, Chen F (2015) pH-Responsive drug-delivery systems. Chem—An Asian J 10:284–305. doi:10.1002/asia.201402715 CrossRef

Titel: Preparation of thermo and pH-responsive polymer@Au/Fe3O4 core/shell nanoparticles as a carrier for delivery of anticancer agent
verfasst von: Marjan Ghorbani
Hamed Hamishehkar
Naser Arsalani
Ali Akbar Entezami
Publikationsdatum: 01.07.2015
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 7/2015
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-015-3097-z

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