Skip to main content
Fachgebiete
Automobil + Motoren Bauwesen + Immobilien Business IT + Informatik Elektrotechnik + Elektronik Energie + Nachhaltigkeit Finance + Banking Management + Führung Marketing + Vertrieb Maschinenbau + Werkstoffe Versicherung + Risiko
DE
EN
Bücher
Zeitschriften
Themenseiten
Organisationspsychologie Projektmanagement Marketing Smart Manufacturing
Weitere Formate
Podcasts Webinare Technik Webinare Wirtschaft Kongresse Veranstaltungskalender Awards
Jetzt Einzelzugang starten
Zugang für Unternehmen
Referenzkunden
SLX-Digitalkonferenz: Zukunftswerkstatt 2024

Mehr Umsatz mit Top-Kontakten

Am 7. Mai erfahren Sie, wie Vertriebsteams Leadgenerierung, Leadnurturing und Leadstrategien effizient steuern können.
Erweiterte Suche
Anmelden
nach oben
Erschienen in:
Introduction to Nano-Biosensing Kapitel lesen Erstes Kapitel lesen

2011 | OriginalPaper | Buchkapitel

2. Nano-scale Force Spectroscopy Applied to Biological Samples

verfasst von : Sandor Kasas, Charles Roduit, Giovanni Dietler

Erschienen in: Nano-Bio-Sensing

Verlag: Springer New York

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

This chapter covers the field of AFM-based force spectroscopy (FS) as applied to biological samples ranging from single molecules up to cells. After a brief introduction to atomic force microscopy and to the basic physical phenomena that are involved in FS measurements, we describe some FS experiments that have been conducted using biological systems of increasing complexities. Several experiments describing FS analysis of DNA, proteins, polysaccharides, and whole cells are successively presented.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Jetzt informieren
Vorheriges Kapitel Introduction to Nano-Biosensing
Nächstes Kapitel Surface Nano-patterning of Polymers for Mass-Sensitive Biodetection
Literatur
1.
Ashkin, A. (1980) Applications of laser-radiation pressure. Science 210: 1081–1088.CrossRef
2.
Amblard, F., B. Yurke, A. Pargellis, et al. (1996) A magnetic manipulator for studying local rheology and micromechanical properties of biological systems. Review of Scientific Instruments 67: 818–827.CrossRef
3.
Smith, S. B., L. Finzi and C. Bustamante (1992) Direct mechanical measurements of the elasticity of single DNA-molecules by using magnetic-beads. Science 258: 1122–1126.CrossRef
4.
Ishijima, A., T. Doi, K. Sakurada, et al. (1991) Sub-piconewton force fluctuations of actomyosin in vitro. Nature 352: 301–306.CrossRef
5.
Florin, E. L., V. T. Moy and H. E. Gaub (1994) Adhesion forces between individual ligand-receptorpairs. Science 264: 415–417.CrossRef
6.
Binnig, G., C. F. Quate and C. Gerber (1986) Atomic force microscopy. Physical Review Letters 56: 930–933.CrossRef
7.
Kasas, S., L. Alonso, P. Jacquet, et al. (2010) Microcontroller-driven fluid-injection system for atomic force microscopy. Review of Scientific Instruments 81.
8.
Cappella, B. and G. Dietler (1999) Force-distance curves by atomic force microscopy. Surface Science Reports 34: 1–104.CrossRef
9.
EssevazRoulet, B., U. Bockelmann and F. Heslot (1997) Mechanical separation of the complementary strands of DNA. Proceedings of the National Academy of Sciences of the United States of America 94: 11935–11940.CrossRef
10.
Yan, H., S. H. Park, G. Finkelstein, et al. (2003) DNA-templated self-assembly of protein arrays and highly conductive nanowires. Science 301: 1882–1884.CrossRef
11.
Liu, Q. H., L. M. Wang, A. G. Frutos, et al. (2000) DNA computing on surfaces. Nature 403: 175–179.CrossRef
12.
Rief, M., H. Clausen-Schaumann and H. E. Gaub (1999) Sequence-dependent mechanics of single DNA molecules. Nature Structural Biology 6: 346–349.CrossRef
13.
Krautbauer, R., M. Rief and H. E. Gaub (2003) Unzipping DNA oligomers. Nano Letters 3: 493–496.CrossRef
14.
Cluzel, P., A. Lebrun, C. Heller, et al. (1996) DNA: an extensible molecule. Science 271: 792–794.
15.
Smith, S.B., Y. Cui, and C. Bustamante (1996) Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Science 271: 795-799.
16.
Cocco, S., J. Yan, J. Léger, D. Chatenay and J.F. Marko (2004) Overstretching and force-driven strand separation of double-helix DNA. Phys. Rev. E 70: 011910.
17.
Sulkowska, J. I. and M. Cieplak (2007) Mechanical stretching of proteins – a theoretical survey of the Protein Data Bank. Journal of Physics-Condensed Matter 19.
18.
Bizzarri, A. R. and S. Cannistraro (2009) Atomic force spectroscopy in biological complex formation: strategies and perspectives. Journal of Physical Chemistry B 113: 16449–16464.CrossRef
19.
Livadaru, L., R. R. Netz and H. J. Kreuzer (2003) Stretching response of discrete semiflexible polymers. Macromolecules 36: 3732–3744.CrossRef
20.
Bustamante, C., J. F. Marko, E. D. Siggia, et al. (1994) Entropic elasticity of lambda-phage DNA. Science 265: 1599–1600.CrossRef
21.
West, D. K., D. J. Brockwell, P. D. Olmsted, et al. (2006) Mechanical resistance of proteins explained using simple molecular models. Biophysical Journal 90: 287–297.CrossRef
22.
Schlierf, M. and M. Rief (2005) Temperature softening of a protein in single-molecule experiments. Journal of Molecular Biology 354: 497–503.CrossRef
23.
Dougan, L., G. Feng, H. Lu, et al. (2008) Solvent molecules bridge the mechanical unfolding transition state of a protein. Proceedings of the National Academy of Sciences of the United States of America 105: 3185–3190.CrossRef
24.
Evans, E. and K. Ritchie (1997) Dynamic strength of molecular adhesion bonds. Biophysical Journal 72: 1541–1555.CrossRef
25.
Carrion-Vazquez, M., P. E. Marszalek, A. F. Oberhauser, et al. (1999) Atomic force microscopy captures length phenotypes in single proteins. Proceedings of the National Academy of Sciences of the United States of America 96: 11288–11292.CrossRef
26.
Brockwell, D. J., E. Paci, R. C. Zinober, et al. (2003) Pulling geometry defines the mechanical resistance of a beta-sheet protein. Nature Structural Biology 10: 731–737.CrossRef
27.
Marszalek, P. E., H. B. Li, A. F. Oberhauser, et al. (2002) Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM. Proceedings of the National Academy of Sciences of the United States of America 99: 4278–4283.CrossRef
28.
Oberhauser, A. F., P. K. Hansma, M. Carrion-Vazquez, et al. (2001) Stepwise unfolding of titin under force-clamp atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America 98: 468–472.CrossRef
29.
Fernandez, J. M. and H. B. Li (2004) Force-clamp spectroscopy monitors the folding trajectory of a single protein. Science 303: 1674–1678.CrossRef
30.
Garcia-Manyes, S., J. Brujic, C. L. Badilla, et al. (2007) Force-clamp spectroscopy of single-protein monomers reveals the individual unfolding and folding pathways of I27 and ubiquitin. Biophysical Journal 93: 2436–2446.CrossRef
31.
Bullard, B., T. Garcia, V. Benes, et al. (2006) The molecular elasticity of the insect flight muscle proteins projectin and kettin. Proceedings of the National Academy of Sciences of the United States of America 103: 4451–4456.CrossRef
32.
Cao, Y. and H. B. Li (2006) Single molecule force spectroscopy reveals a weakly populated microstate of the FnIII domains of tenascin. Journal of Molecular Biology 361: 372–381.CrossRef
33.
Rief, M., J. Pascual, M. Saraste, et al. (1999) Single molecule force spectroscopy of spectrin repeats: low unfolding forces in helix bundles. Journal of Molecular Biology 286: 553–561.CrossRef
34.
Brujic, J., R. I. Z. Hermans, S. Garcia-Manyes, et al. (2007) Dwell-time distribution analysis of polyprotein unfolding using force-clamp spectroscopy. Biophysical Journal 92: 2896–2903.CrossRef
35.
Brown, A. E. X., R. I. Litvinov, D. E. Discher, et al. (2007) Forced unfolding of coiled-coils in fibrinogen by single-molecule AFM. Biophysical Journal 92: L39–L41.CrossRef
36.
Rief, M., M. Gautel, F. Oesterhelt, et al. (1997) Reversible unfolding of individual titin immunoglobulin domains by AFM. Science 276: 1109–1112.CrossRef
37.
Linke, W. A. and A. Grutzner (2008) Pulling single molecules of titin by AFM – recent advances and physiological implications. Pflugers Archiv-European Journal of Physiology 456: 101–115.CrossRef
38.
Schwesinger, F., R. Ros, T. Strunz, et al. (2000) Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates. Proceedings of the National Academy of Sciences of the United States of America 97: 9972–9977.CrossRef
39.
Lee, C. K., Y. M. Wang, L. S. Huang, et al. (2007) Atomic force microscopy: determination of unbinding force, off rate and energy barrier for protein-ligand interaction. Micron 38: 446–461.CrossRef
40.
Yersin, A., H. Hirling, P. Steiner, et al. (2003) Interactions between synaptic vesicle fusion proteins explored by atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America 100: 8736–8741.CrossRef
41.
Rief, M., F. Oesterhelt, B. Heymann, et al. (1997) Single molecule force spectroscopy on polysaccharides by atomic force microscopy. Science 275: 1295–1297.CrossRef
42.
Marszalek, P. E., H. B. Li and J. M. Fernandez (2001) Fingerprinting polysaccharides with single-molecule atomic force microscopy. Nature Biotechnology 19: 258–262.CrossRef
43.
Sletmoen, M., G. Maurstad, P. Sikorski, et al. (2003) Characterisation of bacterial polysaccharides: steps towards single-molecular studies. Carbohydrate Research 338: 2459–2475.CrossRef
44.
Abu-Lail, N. I. and T. A. Camesano (2003) Polysaccharide properties probed with atomic force microscopy. Journal of Microscopy-Oxford 212: 217–238.CrossRefMathSciNet
45.
Ikai, A., R. Afrin, A. Itoh, et al. (2002) Force measurements for membrane protein manipulation. Colloids and Surfaces B-Biointerfaces 23: 165–171.CrossRef
46.
Muller, D. J., M. Krieg, D. Alsteens, et al. (2009) New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells. Current Opinion in Biotechnology 20: 4–13.CrossRef
47.
Verbelen, C. and Y. F. Dufrene (2009) Direct measurement of Mycobacterium–fibronectin interactions. Integrative Biology 1: 296–300.CrossRef
48.
Roduit, C., G. van der Goot, P. de Los Rios, et al. (2008) Elastic Membrane Heterogeneity of Living Cells Revealed by Stiff Nanoscale Membrane Domains. Biophysical Journal 94: 1521–1532.
49.
Carrion-Vazquez, M., A. F. Oberhauser, T. E. Fisher, et al. (2000) Mechanical design of proteins-studied by single-molecule force spectroscopy and protein engineering. Progress in Biophysics and Molecular Biology 74: 63–91.CrossRef
50.
Greenleaf, W. J., M. T. Woodside and S. M. Block (2007) High-resolution, single-molecule measurements of biomolecular motion. Annual Review of Biophysics and Biomolecular Structure 36: 171–190.CrossRef
51.
Ikai, A. and R. Afrin (2003) Toward mechanical manipulations of cell membranes and membrane proteins using an atomic force microscope – an invited review. Cell Biochemistry and Biophysics 39: 257–277.CrossRef
52.
Puchner, E. M. and H. E. Gaub (2009) Force and function: probing proteins with AFM-based force spectroscopy. Current Opinion in Structural Biology 19: 605–614.CrossRef
53.
Afrin, R. and A. Ikai (2006) Force profiles of protein pulling with or without cytoskeletal links studied by AFM. Biochemical and Biophysical Research Communications 348: 238–244.CrossRef
Metadaten
Titel
Nano-scale Force Spectroscopy Applied to Biological Samples
verfasst von
Sandor Kasas
Charles Roduit
Giovanni Dietler
Copyright-Jahr
2011
Verlag
Springer New York
Buch
Nano-Bio-Sensing

Print ISBN: 978-1-4419-6168-6

Electronic ISBN: 978-1-4419-6169-3

Copyright-Jahr: 2011

DOI
https://doi.org/10.1007/978-1-4419-6169-3_2

Neuer Inhalt

    Bildnachweise