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Erschienen in:
2011 | OriginalPaper | Buchkapitel
Opto-Hydrodynamic Trapping for Multiaxial Single-Cell Biomechanics
verfasst von : Sean S. Kohles
Erschienen in: Advances in Cell Mechanics
Verlag: Springer Berlin Heidelberg
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In this chapter, a unique experimental platform is described for singlecell biomechanics. An optical tweezer with minimal applied laser power has successfully suspended biologic cells at the geometric center of a microfluidic crossjunction. The resulting flow environment creates a unique multiaxial load application to isolated cells with site-specific normal and shear stresses resulting in a physically extended state. Computational fluid dynamics combined with multiphysics modeling has characterized the fluid environment and solid cellular strain response in three-dimensions to accompany experimental cell stimulation. These models will guide future microfluidic experiments as well as provide a framework for characterizing cytoskeletal structures influencing the stress on strain response.