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2015 | OriginalPaper | Buchkapitel

Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples

verfasst von : Di Wu, Michael P. Gantier

Verlag: Springer New York

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Abstract

microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.

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Titel: Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples
verfasst von: Di Wu
Michael P. Gantier
Verlag: Springer New York
DOI: https://doi.org/10.1007/7651_2015_239

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Abstract

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