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2011 | OriginalPaper | Buchkapitel

4. Surface Plasmon Resonance on Nanoscale Organic Films

verfasst von : Willem M. Albers, Inger Vikholm-Lundin

Erschienen in: Nano-Bio-Sensing

Verlag: Springer New York

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Abstract

Over the past 20 years, surface plasmon resonance (SPR) has evolved into a very versatile detection method, particularly in bioscience applications. Not only the scientific literature has greatly expanded, but also the various commercial vendors of instrumentation, detection chips, and reagents have emerged. In the scientific sphere, the accent lies more and more on fabrication of nanostructures with interesting optical behavior (plasmonics), while in the R&D area, there are many new miniaturization efforts and combination with other detection methods, such as electrochemistry and quartz crystal microbalance (QCM). The present chapter will focus on the latest developments in making functional biochemical coatings for SPR detection as well as will review the basic theory behind the detection techniques.

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Fußnoten
1
The extinction (E) of a thin film is calculated by the usual relation: \(E = \chi.d = - \ln \left( T \right) = - \ln \left( {I/I_0 } \right)\), where I 0 is the intensity of the incident beam and I that of the transmitted light, T being the transmittance of the film.
 
2
In the ellipsometric literature, the convention \(\hat n = n - {\rm{j}}k,\) is often used, but then the k-values are expressed as negative numbers.
 
3
With the use of hydrogel layers on the gold surface, the SPR method is close to affinity chromatography in which the adsorption and desorption can be monitored directly, instead of monitoring the analyte released from the surface with a down-stream detector.
 
4
In fact, with surface concentrations for x and b, the term (ax) may not be directly used, because a is in mol/L and b in mol/dm2. Thus, a condition of depletion of a should be checked (see Chap. 5 of [7] for a mathematical evaluation).
 
5
Svante Arrhenius (1859–1927) was a Swedish scientist, and one of the founders of the science of physical chemistry: originally he was a physicist, but he is often regarded as a chemist. The Arrhenius equation he developed based on the work by J. H. van‘t Hoff. He was also the first to develop a theory of the greenhouse effect caused by carbon dioxide.
 
6
A recent scan of the literature readily reveals that most studies with direct detection using SPR instrumentation move in the low nanomolar range [18].
 
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Metadaten
Titel
Surface Plasmon Resonance on Nanoscale Organic Films
verfasst von
Willem M. Albers
Inger Vikholm-Lundin
Copyright-Jahr
2011
Verlag
Springer New York
DOI
https://doi.org/10.1007/978-1-4419-6169-3_4

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