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Journal of Nanoparticle Research

Erschienen in:

01.05.2015 | Research Paper

Synthesis of magnetic thermosensitive microcontainers for enzyme immobilization

verfasst von: Jianzhi Wang, Guanghui Zhao, Xinyu Wang, Xiaomen Peng, Yanfeng Li

Erschienen in: Journal of Nanoparticle Research | Ausgabe 5/2015

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Abstract

We present a new approach for the fabrication of magnetic thermoresponsive polymer microcapsules with mobile magnetic spherical cores. The microcontainers form fried-egg-like structures with a polymer shell layer of 50 nm due to the existence of hollow cavities. The microcontainers undergo a temperature-induced volume phase transition upon changing the temperature and present an impressive magnetic response. The magnetic saturation of these smart microcontainers (42 emu/g) is high enough to meet most requirements of bioapplications. To further investigate the potential application of these smart microcontainers in biotechnology, Candida rugosa lipase was selected for the enzyme immobilization process. The immobilized lipase exhibited excellent thermal stability and reusability in comparison with the free enzyme. The adsorption/release of the lipase from the microcontainers can be controlled by the environmental temperature and magnetic force, thus, offering new potential applications such as in controlled drug delivery, bioseparation, and catalysis.

Vorheriger Artikel Kinematics of gold nanoparticles manipulation in situ transmission electron microscopy

Nächster Artikel Nanostructured mesoporous silica: new perspectives for fighting antimicrobial resistance

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Bae Y, Fukushima S, Harada A, Kataoka K (2003) Design of environment-sensitive supramolecular assemblies for intracellular drug delivery: polymeric micelles that are responsive to intracellular pH change. Angew Chem Int Ed 42(38):4640–4643CrossRef

Bilalis P, Chatzipavlidis A, Tziveleka LA, Boukos N, Kordas G (2012a) Nanodesigned magnetic polymer containers for dual stimuli actuated drug controlled release and magnetic hyperthermia mediation. J Mater Chem 22(27):13451–13454CrossRef

Bilalis P, Efthimiadou EK, Chatzipavlidis A, Boukos N, Kordas GC (2012b) Multi-responsive polymeric microcontainers for potential biomedical applications: synthesis and functionality evaluation. Polym Int 61(6):888–894CrossRef

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1):248–254CrossRef

Carregal-Romero S, Buurma NJ, Pérez-Juste J, Liz-Marzán LM, Hervés P (2010) Catalysis by Au@ pNIPAM nanocomposites: effect of the cross-linking density. Chem Mater 22(10):3051–3059CrossRef

Chang B, Sha X, Guo J, Jiao YF, Wang CC, Yang WL (2011) Thermo and pH dual responsive, polymer shell coated, magnetic mesoporous silica nanoparticles for controlled drug release. J Mater Chem 21(25):9239–9247CrossRef

Chen JP, Su DR (2001) Latex particles with thermo-flocculation and magnetic properties for immobilization of α-chymotrypsin. Biotechnol Prog 17(2):369–375CrossRef

Chen LB, Zhang F, Wang CC (2009) Rational synthesis of magnetic thermosensitive microcontainers as targeting drug carriers. Small 5(5):621–628CrossRef

Chen J, Liu M, Chen C, Gong HH, Gao CM (2011) Synthesis and characterization of silica nanoparticles with well-defined thermoresponsive PNIPAM via a combination of RAFT and click chemistry. ACS Appl Mater Interfaces 3(8):3215–3223CrossRef

Deng YH, Yang WL, Wang CC, Fu SK (2003) A novel approach for preparation of thermoresponsive polymer magnetic microspheres with core-shell structure. Adv Mater 15(20):1729–1732CrossRef

Deng H, Li X, Peng Q, Wang X, Chen JP, Li YD (2005) Monodisperse magnetic single-crystal ferrite microspheres. Angew Chem 117(18):2842–2845CrossRef

Dubbert J, Honold T, Pedersen JS, Radulescu A, Drechsler M, Karg M, Richtering W (2014) How hollow are thermoresponsive hollow nanogels? Macromolecules 47:8700–8708CrossRef

Gao H, Yang W, Min K, Zha LS, Wang CC, Fu SK (2005) Thermosensitive poly (N-isopropylacrylamide) nanocapsules with controlled permeability. Polymer 46(4):1087–1093CrossRef

Hou C, Zhu H, Wu DM, Li YJ, Hou K, Jiang Y, Li YF (2014) Immobilized lipase on macroporous polystyrene modified by PAMAM-dendrimer and their enzymatic hydrolysis. Process Biochem 49:244–249CrossRef

Khosroshahi ME, Ghazanfari L (2012) Synthesis and functionalization of SiO₂ coated Fe₃O₄ nanoparticles with amine groups based on self-assembly. Mater Sci Eng, C 32(5):1043–1049CrossRef

Lei L, Liu X, Li YF, Cui YJ, Yang Y, Qin GR (2011) Study on synthesis of poly (GMA)-grafted Fe₃O₄/SiO_X magnetic nanoparticles using atom transfer radical polymerization and their application for lipase immobilization. Mater Chem Phys 125:866–871CrossRef

Li P, Zhu AM, Liu QL, Zhang QG (2008) Fe₃O₄/poly (N-isopropylacrylamide)/chitosan composite microspheres with multiresponsive properties. Ind Eng Chem Res 47(20):7700–7706CrossRef

Li Q, Zhang L, Bai L, Zhang ZB, Zhu J, Zhou NC, Cheng ZP, Zhu XL (2011) Multistimuli-responsive hybrid nanoparticles with magnetic core and thermoresponsive fluorescence-labeled shell via surface-initiated RAFT polymerization. Soft Matter 7(15):6958–6966CrossRef

Liu X, Lei L, Li YF, Zhu H, Cui YJ, Hu HY (2011) Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase. Biochem Eng J 56(3):142–149CrossRef

Luo B, Song XJ, Zhang F, Xia A, Yang WL, Hu JH, Wang CC (2009) Multi-functional thermosensitive composite microspheres with high magnetic susceptibility based on magnetite colloidal nanoparticle clusters. Langmuir 26(3):1674–1679CrossRef

Qin L, He XW, Yuan X, Li WY, Zhang YK (2011) Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins. Anal Bioanal Chem 399(10):3375–3385CrossRef

Qu L, Shi G (2004) Hollow microstructures of polypyrrole doped by poly (styrene sulfonic acid). J Polym Sci, Part A 42(13):3170–3177CrossRef

Shamim N, Hong L, Hidajat K, Uddin MS (2007) Thermosensitive polymer coated nanomagnetic particles for separation of bio-molecules. Sep Purif Technol 53(2):164–170CrossRef

Shchukin DG, Möhwald H (2005) Urea photosynthesis inside polyelectrolyte capsules: effect of confined media. Langmuir 21(12):5582–5587CrossRef

Stöber W, Fink A, Bohn E (1968) Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci 26(1):62–69CrossRef

Tong W, Song X, Gao C (2012) Layer-by-layer assembly of microcapsules and their biomedical applications. Chem Soc Rev 41(18):6103–6124CrossRef

Wang HD, Chu LY, Yu XQ, Xie R, Yang M, Xu D, Zhang J, Lin H (2007) Thermosensitive affinity behavior of poly (N-isopropylacrylamide) hydrogels with β-cyclodextrin moieties. Ind Eng Chem Res 46(5):1511–1518CrossRef

Wang JZ, Zhao GH, Li YF, Liu X, Hou PP (2013) Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers. Appl Microbiol Biotechnol 97(2):681–692CrossRef

Ward MA, Georgiou TK (2011) Thermoresponsive polymers for biomedical applications. Polymers 3(3):1215–1242CrossRef

Wu T, Zhang YF, Wang XF, Liu SY (2007) Fabrication of hybrid silica nanoparticles densely grafted with thermoresponsive poly (N-isopropylacrylamide) brushes of controlled thickness via surface-initiated atom transfer radical polymerization. Chem Mater 20(1):101–109CrossRef

Wu F, Li Q, Zhang X, Liu L, Wu SX, Sun DP, Li FS (2012) Fabrication and characterization of thermo-sensitive magnetic polymer composite nanoparticles. J Magn Magn Mater 324(7):1326–1330CrossRef

Xie W, Ma N (2010) Enzymatic transesterification of soybean oil by using immobilized lipase on magnetic nano-particles. Biomass Bioenergy 34(6):890–896CrossRef

Xu SF, Lu HZ, Zheng XW, Chen LX (2013) Stimuli-responsive molecularly imprinted polymers: versatile functional materials. J Mater Chem C 1(29):4406–4422CrossRef

Zhang F, Wang CC (2008) Preparation of thermoresponsive core-shell polymeric microspheres and hollow PNIPAM microgels. Colloid Polym Sci 286(8–9):889–895CrossRef

Zhang F, Wang CC (2009) Preparation of P(NIPAM-co-AA) microcontainers surface-anchored with magnetic nanoparticles. Langmuir 25(14):8255–8262CrossRef

Zhao GH, Li YF, Wang JZ, Zhu H (2011) Reversible immobilization of glucoamylase onto magnetic carbon nanotubes functionalized with dendrimer. Appl Microbiol Biotechnol 91(3):591–601CrossRef

Zhu H, Hou C, Li YJ, Zhao GH, Liu X, Hou K, Li YF (2013) One-pot solvothermal synthesis of highly water-dispersible size-tunable functionalized magnetite nanocrystal clusters for lipase immobilization. Chem Asian J 8(7):1447–1454CrossRef

Titel: Synthesis of magnetic thermosensitive microcontainers for enzyme immobilization
verfasst von: Jianzhi Wang
Guanghui Zhao
Xinyu Wang
Xiaomen Peng
Yanfeng Li
Publikationsdatum: 01.05.2015
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 5/2015
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-015-2986-5

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