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Erschienen in:
2015 | OriginalPaper | Buchkapitel
Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS
verfasst von : Thomas Dertinger, Steffen Rüttinger
Erschienen in: Advanced Photon Counting
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Abstract
This chapter focuses on two advanced fluorescence correlation spectroscopy (FCS) methods; fluorescence lifetime correlation spectroscopy (FLCS) and dual-focus FCS (2fFCS). We decided to put our focus on a detailed discussion of these two – and in our eyes well-merited – advanced methods, rather than giving an overview over the broad variety of advanced FCS methods that would consequently lack detail and leave the reader rather uneducated on all these methods. For this reason we had to exclude some candidates that would very well deserve the same amount of attention as the methods that we chose to focus on. Amongst these candidates camera-FCS, Bayes-FCS, and scanning-FCS are to be kept on the radar for sure.
The great benefit of FLCS is that it provides a general tool that allows filtering for sub-populations, afterpulsing-artifacts, background effects, and basically anything that can be distinguished by its fluorescence lifetime. Complementarily, 2fFCS has brought a new level of accuracy to the table that has been previously reached only by complementary methods such as for example pulsed-field gradient NMR.