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2020 | OriginalPaper | Buchkapitel

8. Analytical Protocols in Phycobiliproteins Analysis

verfasst von : Milan R. Nikolic, Simeon Minic, Mirjana Macvanin, Dragana Stanic-Vucinic, Tanja Cirkovic Velickovic

Erschienen in: Pigments from Microalgae Handbook

Verlag: Springer International Publishing

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Abstract

The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (10⁵ to 10⁶) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.

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Titel: Analytical Protocols in Phycobiliproteins Analysis
verfasst von: Milan R. Nikolic
Simeon Minic
Mirjana Macvanin
Dragana Stanic-Vucinic
Tanja Cirkovic Velickovic
Verlag: Springer International Publishing
Buch: Pigments from Microalgae Handbook
Print ISBN: 978-3-030-50970-5

Electronic ISBN: 978-3-030-50971-2

Copyright-Jahr: 2020
DOI: https://doi.org/10.1007/978-3-030-50971-2_8