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17.01.2020 | Original Research

Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma

verfasst von: Chen Zhang, Jingjie Cao, Shaozhen Zhao, Honglin Luo, Zhiwei Yang, Miguel Gama, Quanchao Zhang, Dan Su, Yizao Wan

Erschienen in: Cellulose | Ausgabe 5/2020

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Abstract

In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.

Vorheriger Artikel Cellulose/biopolymer/Fe3O4 hydrogel microbeads for dye and protein adsorption

Nächster Artikel Synthesis of polyamide-6@cellulose microfilms grafted with N-vinylcaprolactam using gamma-rays and loading of antimicrobial drugs

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Titel: Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
verfasst von: Chen Zhang
Jingjie Cao
Shaozhen Zhao
Honglin Luo
Zhiwei Yang
Miguel Gama
Quanchao Zhang
Dan Su
Yizao Wan
Publikationsdatum: 17.01.2020
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 5/2020
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-020-02979-0

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