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Journal of Nanoparticle Research
Erschienen in: Journal of Nanoparticle Research 2/2014

01.02.2014 | Research Paper

Carbodiimide-mediated immobilization of serratiopeptidase on amino-, carboxyl-functionalized magnetic nanoparticles and characterization for target delivery

verfasst von: Sandeep Kumar, Asim K. Jana, Mithu Maiti, Isha Dhamija

Erschienen in: Journal of Nanoparticle Research | Ausgabe 2/2014

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Abstract

A hybrid biomaterial of serratiopeptidase enzyme was prepared with magnetic nanoparticles (MNPs) via carboxyl and amino-functionalization and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for direct immobilization. The average size of prepared MNPs was found to be 15.05 ± 3.06 nm. Attachment of amino and carboxyl groups was confirmed by Fourier transform infrared spectroscopy. X-ray diffraction confirmed the purity and phase integrity of Fe3O4. The MNPs and enzyme-loaded-MNPs (EMNPs) were of saturation magnetization 58 and 50 emu g−1, respectively. Thermogravimetric analysis of EDC-MNPs and EMNPs showed the presence of organic coating over MNPs. Serratiopeptidase immobilized on amino-functionalized magnetic nanoparticles showed loss of enzyme activity due to crosslinking of enzyme, while serratiopeptidase immobilized on carboxyl-functionalized magnetic nanoparticles was better and gave 115.78 mg protein g−1 MNPs, enzyme loading 168.32 U g−1 MNPs at optimized MNPs-to-enzyme ratio 1.0 mg mg−1. In vitro and in vivo studies showed that EMNPs with magnetic targeting is more effective in drug permeation and reduction in edema than free enzyme.
Vorheriger Artikel Effects of polyaniline coating of cryptomelane-type KMn8O16 on electrochemical performance for lithium-ion batteries
Nächster Artikel Hydrogen-induced Ostwald ripening of cobalt nanoparticles on carbon nanotubes

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Metadaten
Titel
Carbodiimide-mediated immobilization of serratiopeptidase on amino-, carboxyl-functionalized magnetic nanoparticles and characterization for target delivery
verfasst von
Sandeep Kumar
Asim K. Jana
Mithu Maiti
Isha Dhamija
Publikationsdatum
01.02.2014
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 2/2014
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-013-2233-x

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