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Journal of Nanoparticle Research

Erschienen in:

01.05.2014 | Research Paper

Cellular uptake induced biotoxicity of surface-modified CdSe quantum dots

verfasst von: Shilpa Sanwlani, Kamla Rawat, Meena Pal, Himadri B. Bohidar, Anita Kamra Verma

Erschienen in: Journal of Nanoparticle Research | Ausgabe 5/2014

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Abstract

Cellular uptake of quantum dots (QDs) by cells is of utmost importance for establishing QDs as biostable fluorescent markers that facilitate early diagnosis and detection of cancer. The surface states of QDs are critical to enhance the cellular uptake. Biocompatible CDSe QDs were synthesized using mercaptopropionic acid, amino-ethanethiol HCl, cyltrimethylammonium bromide, dodecyltrimethylammonium bromide, tetrabutylammonium iodide (TBAI), and sodium dodecyl sulfate were functionalized using ligand-exchange method. Cytocompatibility and cellular uptake of QDs were evaluated in human embryonic kidney cells (HEK-29), and breast cancer cells (MCF-7) as reduced cytotoxicity is desirable for biological applications. Approximately, 60 % cytotoxicity was observed in all surface-coated QDs and QD100 in 72 h in both the cell lines, except TBAI that indicated 30 % cytotoxicity in 72 h, and only 10 % in 24 h. Glutathione, the detoxifying molecule, is detrimental for understanding the oxidative stress of the cell. The QDs showed enhanced Glutathione-S-transferase (GST) activity in the MCF-7 cell line. In HEK, CdSe per se was also able to induce a high level of GST. QDs toxicity may either be related to the induction of reactive oxygen species or the direct release of metal ions. Optimization of QDs in terms of quantification and DNA damage is imperative for realistic biological applications.

Vorheriger Artikel Improved ferromagnetic properties of electrospun NiFe2O4 with tunable morphology: from multiparticle-chain to single-particle-chain

Nächster Artikel Cellular uptake and distribution of graphene oxide coated with layer-by-layer assembled polyelectrolytes

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Titel: Cellular uptake induced biotoxicity of surface-modified CdSe quantum dots
verfasst von: Shilpa Sanwlani
Kamla Rawat
Meena Pal
Himadri B. Bohidar
Anita Kamra Verma
Publikationsdatum: 01.05.2014
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 5/2014
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2382-6

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